(Circular) (Six-base) MAPSORT of: pFN21AE2769.seq Check: 2380 from: 1 to: 6118 pFN21AE2769 6118 bp With 182 enzymes: SgfI * July 4, 2020 18:17 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3928 278 Size: 53 83 186 936 2392 2468 Fragments arranged by size: 2468 2392 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3461 1937 Size: 1524 4594 Acc65I G'GTAC_C Cuts at: 3361 3439 3361 Size: 78 6040 AclI AA'CG_TT Cuts at: 1563 4245 4618 1563 Size: 2682 373 3063 Fragments arranged by size: 3063 2682 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2777 2813 4181 5565 5833 1632 Size: 1145 36 1368 1384 268 1917 Fragments arranged by size: 1917 1384 1368 1145 268 36 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5921 AflIII A'CryG_T Cuts at: 1203 3168 5017 5994 1203 Size: 1965 1849 977 1327 Fragments arranged by size: 1965 1849 1327 977 AgeI A'CCGG_T Cuts at: 4949 4949 Size: 6118 AhdI GACnn_n'nnGTC Cuts at: 4847 4847 Size: 6118 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6118 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6118 AlwNI CAG_nnn'CTG Cuts at: 2179 3052 3861 5433 2179 Size: 873 809 1572 2864 Fragments arranged by size: 2864 1572 873 809 ApaLI G'TGCA_C Cuts at: 4175 5331 4175 Size: 1156 4962 ApoI r'AATT_y Cuts at: 1493 3427 3653 3805 5914 1493 Size: 1934 226 152 2109 1697 Fragments arranged by size: 2109 1934 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4672 160 Size: 872 3640 1606 Fragments arranged by size: 3640 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6118 AvaI C'yCGr_G Cuts at: 1870 1943 3443 1870 Size: 73 1500 4545 Fragments arranged by size: 4545 1500 73 BamHI G'GATC_C Cuts at: 3448 3815 3448 Size: 367 5751 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2031 2200 2323 2347 3361 Size: 325 217 627 244 169 123 24 1014 Cuts at: 3361 3439 4894 618 Size: 78 1455 1842 Fragments arranged by size: 1842 1455 1014 627 325 244 217 169 123 78 24 BanII G_rGCy'C Cuts at: 1391 2384 2732 2994 3190 3437 1391 Size: 993 348 262 196 247 4072 Fragments arranged by size: 4072 993 348 262 247 196 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2071 928 Size: 971 172 4975 Fragments arranged by size: 4975 971 172 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4309 4343 1903 Size: 34 2372 34 3678 Fragments arranged by size: 3678 2372 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4010 5220 4010 Size: 1210 4908 BclI T'GATC_A Cuts at: 1548 1548 Size: 6118 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2134 2321 4729 Size: 107 122 71 631 605 462 187 2408 Cuts at: 4729 136 Size: 1525 Fragments arranged by size: 2408 1525 631 605 462 187 122 107 71 BglII A'GATC_T Cuts at: 6113 6113 Size: 6118 BlpI GC'TnA_GC Cuts at: 3535 3535 Size: 6118 Bme1580I G_kGCm'C Cuts at: 1790 2352 3042 4179 5335 1790 Size: 562 690 1137 1156 2573 Fragments arranged by size: 2573 1156 1137 690 562 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6118 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3256 3849 4807 Size: 416 53 83 399 24 1839 593 958 Cuts at: 4807 442 Size: 1753 Fragments arranged by size: 1839 1753 958 593 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6118 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3204 3204 Size: 6118 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2518 2867 2908 3018 4778 1239 Size: 1279 349 41 110 1760 2579 Fragments arranged by size: 2579 1760 1279 349 110 41 Bpu10I CC'TnA_GC Cuts at: 2036 2036 Size: 6118 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4243 5108 5406 4243 Size: 865 298 4955 Fragments arranged by size: 4955 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2172 4781 882 Size: 617 673 2609 2219 Fragments arranged by size: 2609 2219 673 617 BsaAI yAC'GTr Cuts at: 493 1204 3169 3909 493 Size: 711 1965 740 2702 Fragments arranged by size: 2702 1965 740 711 BsaBI GATnn'nnATC Cuts at: 6112 6112 Size: 6118 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2046 3925 4307 4942 Size: 53 83 186 936 513 1879 382 635 Cuts at: 4942 275 Size: 1451 Fragments arranged by size: 1879 1451 936 635 513 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2294 3084 4551 4949 5223 5370 5781 2294 Size: 790 1467 398 274 147 411 2631 Fragments arranged by size: 2631 1467 790 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5893 Fragments arranged by size: 5893 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2348 1186 Size: 1162 4956 BseYI C'CCAG_C Cuts at: 2274 3042 3074 5321 5817 2274 Size: 768 32 2247 496 2575 Fragments arranged by size: 2575 2247 768 496 32 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3029 3333 3357 1163 Size: 573 1293 304 24 3924 Fragments arranged by size: 3924 1293 573 304 24 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3568 4329 5357 1540 Size: 275 186 1567 761 1028 2301 Fragments arranged by size: 2301 1567 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2994 3190 4179 4264 5335 2994 Size: 196 989 85 1071 3777 Fragments arranged by size: 3777 1071 989 196 85 BsmI GAATG_Cn' Cuts at: 2616 3629 3722 2616 Size: 1013 93 5012 Fragments arranged by size: 5012 1013 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2352 2384 2732 2994 3042 3190 3437 Size: 399 562 32 348 262 48 148 247 Cuts at: 3437 4179 4264 5335 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 562 399 348 262 247 148 85 48 32 BspEI T'CCGG_A Cuts at: 5781 5781 Size: 6118 BspHI T'CATG_A Cuts at: 4007 5737 4007 Size: 1730 4388 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3473 844 Size: 2629 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3183 4005 1394 Size: 429 1360 822 3507 Fragments arranged by size: 3507 1360 822 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4613 4787 65 Size: 1183 3365 174 1396 Fragments arranged by size: 3365 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4762 4949 1700 Size: 110 2952 187 2869 Fragments arranged by size: 2952 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6118 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6118 BssSI C'ACGA_G Cuts at: 4178 5190 4178 Size: 1012 5106 BstAPI GCAn_nnn'nTGC Cuts at: 794 2611 3052 794 Size: 1817 441 3860 Fragments arranged by size: 3860 1817 441 BstBI TT'CG_AA Cuts at: 2814 4922 2814 Size: 2108 4010 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6118 BstYI r'GATC_y Cuts at: 1972 3448 3815 4200 4217 5669 5755 5767 6113 Size: 1476 367 385 17 1452 86 12 346 Cuts at: 6113 1972 Size: 1977 Fragments arranged by size: 1977 1476 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2682 2697 2682 Size: 15 6103 BtgI C'CryG_G Cuts at: 513 1065 2400 3102 513 Size: 552 1335 702 3529 Fragments arranged by size: 3529 1335 702 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5379 Fragments arranged by size: 5379 683 56 BtsI GCAGTG_nn' Cuts at: 2637 3634 4428 4448 2637 Size: 997 794 20 4307 Fragments arranged by size: 4307 997 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6118 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6083 DraI TTT'AAA Cuts at: 3422 3422 Size: 6118 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5125 809 Size: 507 3809 1802 Fragments arranged by size: 3809 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3060 3565 4454 Size: 54 1425 50 275 18 1230 505 889 Cuts at: 4454 6083 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1230 889 505 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3565 1537 Size: 275 1753 4090 Fragments arranged by size: 4090 1753 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2093 2372 2468 2918 4048 1353 Size: 740 279 96 450 1130 3423 Fragments arranged by size: 3423 1130 740 450 279 96 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4705 5079 5225 387 Size: 1064 138 3116 374 146 1280 Fragments arranged by size: 3116 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2992 3188 2992 Size: 196 5922 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2518 2777 2813 2867 2908 3018 4181 Size: 393 886 259 36 54 41 110 1163 Cuts at: 4181 4778 5565 5833 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1163 886 787 597 393 268 259 110 54 41 36 EcoNI CCTnn'n_nnAGG Cuts at: 2449 2449 Size: 6118 EcoO109I rG'GnC_Cy Cuts at: 2010 2010 Size: 6118 EcoRI G'AATT_C Cuts at: 3427 3805 3427 Size: 378 5740 FspI TGC'GCA Cuts at: 4624 4624 Size: 6118 HaeII r_GCGC'y Cuts at: 1678 5265 1678 Size: 3587 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2450 2482 3142 3174 4759 Size: 32 135 32 878 32 660 32 1585 Cuts at: 4759 4791 4833 4865 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1585 878 660 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2637 3462 3708 669 Size: 988 281 699 825 246 3079 Fragments arranged by size: 3079 988 825 699 281 246 HindIII A'AGCT_T Cuts at: 748 3331 748 Size: 2583 3535 HpaI GTT'AAC Cuts at: 3708 3708 Size: 6118 KpnI G_GTAC'C Cuts at: 3365 3443 3365 Size: 78 6040 MfeI C'AATT_G Cuts at: 3717 3717 Size: 6118 MluI A'CGCG_T Cuts at: 5994 5994 Size: 6118 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5232 5416 1305 Size: 3927 184 2007 Fragments arranged by size: 3927 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3062 10 Size: 54 1425 343 1230 3066 Fragments arranged by size: 3066 1425 1230 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2467 4076 4435 4594 518 Size: 648 1301 1609 359 159 2042 Fragments arranged by size: 2042 1609 1301 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3052 3074 3534 4211 5359 5604 1606 Size: 1446 22 460 677 1148 245 2120 Fragments arranged by size: 2120 1446 1148 677 460 245 22 NcoI C'CATG_G Cuts at: 513 1065 2400 513 Size: 552 1335 4231 Fragments arranged by size: 4231 1335 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6118 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6118 NotI GC'GGCC_GC Cuts at: 3565 3565 Size: 6118 NspI r_CATG'y Cuts at: 1132 1229 3476 5021 1132 Size: 97 2247 1545 2229 Fragments arranged by size: 2247 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6118 PciI A'CATG_T Cuts at: 5017 5017 Size: 6118 PflMI CCAn_nnn'nTGG Cuts at: 1108 2978 1108 Size: 1870 4248 PfoI T'CCnGG_A Cuts at: 2534 2916 2534 Size: 382 5736 PmeI GTTT'AAAC Cuts at: 3422 3422 Size: 6118 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4187 4219 5700 5732 4187 Size: 32 1481 32 4573 Fragments arranged by size: 4573 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2010 2010 Size: 6118 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6118 PsiI TTA'TAA Cuts at: 3688 3688 Size: 6118 PstI C_TGCA'G Cuts at: 830 3470 830 Size: 2640 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6118 PvuII CAG'CTG Cuts at: 3052 3052 Size: 6118 SacI G_AGCT'C Cuts at: 2994 3190 2994 Size: 196 5922 SalI G'TCGA_C Cuts at: 1936 3460 1936 Size: 1524 4594 SapI GCTCTTCn'nnn_ Cuts at: 2372 2468 2372 Size: 96 6022 SbfI CC_TGCA'GG Cuts at: 3470 3470 Size: 6118 ScaI AGT'ACT Cuts at: 4366 6078 4366 Size: 1712 4406 SfcI C'TryA_G Cuts at: 826 1045 2171 3466 4601 5282 5473 826 Size: 219 1126 1295 1135 681 191 1471 Fragments arranged by size: 1471 1295 1135 1126 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6118 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6118 SmaI CCC'GGG Cuts at: 1872 3445 1872 Size: 1573 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4222 5123 5385 820 Size: 197 926 2279 901 262 1553 Fragments arranged by size: 2279 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3909 493 Size: 3416 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6118 SphI G_CATG'C Cuts at: 1132 3476 1132 Size: 2344 3774 SspI AAT'ATT Cuts at: 5 52 4042 5 Size: 47 3990 2081 Fragments arranged by size: 3990 2081 47 StuI AGG'CCT Cuts at: 1517 2826 1517 Size: 1309 4809 StyI C'CwwG_G Cuts at: 513 1065 2041 2280 2400 3003 3557 513 Size: 552 976 239 120 603 554 3074 Fragments arranged by size: 3074 976 603 554 552 239 120 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2218 2434 3391 4145 4162 4315 4500 Size: 1255 638 216 957 754 17 153 185 Cuts at: 4500 4945 325 Size: 445 1498 Fragments arranged by size: 1498 1255 957 754 638 445 216 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4364 6076 96 Size: 274 80 33 51 1443 2387 1712 138 Fragments arranged by size: 2387 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6078 6110 6078 Size: 32 6086 Tth111I GACn'n_nGTC Cuts at: 1316 2542 3262 1316 Size: 1226 720 4172 Fragments arranged by size: 4172 1226 720 XbaI T'CTAG_A Cuts at: 3454 3454 Size: 6118 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6118 XmaI C'CCGG_G Cuts at: 1870 3443 1870 Size: 1573 4545 XmnI GAAnn'nnTTC Cuts at: 3334 4247 3334 Size: 913 5205 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3926 276 Size: 53 83 186 936 2392 2468 Fragments arranged by size: 2468 2392 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI