(Linear) (Six-base) MAPSORT of: KIEE2769.seq Check: 8461 from: 1 to: 1413 KIEE2769.orf With 182 enzymes: SgfI * July 3, 2020 20:21 .. Acc65I G'GTAC_C Cuts at: 0 1356 1413 Size: 1356 57 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 772 808 1413 Size: 772 36 605 Fragments arranged by size: 772 605 36 AflIII A'CryG_T Cuts at: 0 1163 1413 Size: 1163 250 AlwNI CAG_nnn'CTG Cuts at: 0 174 1047 1413 Size: 174 873 366 Fragments arranged by size: 873 366 174 BanI G'GyrC_C Cuts at: 0 26 195 318 342 1356 1413 Size: 26 169 123 24 1014 57 Fragments arranged by size: 1014 169 123 57 26 24 BanII G_rGCy'C Cuts at: 0 379 727 989 1185 1413 Size: 379 348 262 196 228 Fragments arranged by size: 379 348 262 228 196 BbsI GAAGACnn'nnnn_ Cuts at: 0 66 1413 Size: 66 1347 BglI GCCn_nnn'nGGC Cuts at: 0 129 316 1413 Size: 129 187 1097 Fragments arranged by size: 1097 187 129 Bme1580I G_kGCm'C Cuts at: 0 347 1037 1413 Size: 347 690 376 Fragments arranged by size: 690 376 347 BmrI ACTGGGnnnn_n' Cuts at: 0 1251 1413 Size: 1251 162 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1199 1413 Size: 1199 214 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 513 862 903 1013 1413 Size: 513 349 41 110 400 Fragments arranged by size: 513 400 349 110 41 Bpu10I CC'TnA_GC Cuts at: 0 31 1413 Size: 31 1382 BsaI GGTCTCn'nnnn_ Cuts at: 0 167 1413 Size: 167 1246 BsaAI yAC'GTr Cuts at: 0 1164 1413 Size: 1164 249 BsaHI Gr'CG_yC Cuts at: 0 41 1413 Size: 41 1372 BsaWI w'CCGG_w Cuts at: 0 289 1079 1413 Size: 289 790 334 Fragments arranged by size: 790 334 289 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 343 1413 Size: 343 1070 BseYI C'CCAG_C Cuts at: 0 269 1037 1069 1413 Size: 269 768 32 344 Fragments arranged by size: 768 344 269 32 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1024 1328 1352 1413 Size: 1024 304 24 61 Fragments arranged by size: 1024 304 61 24 BsiHKAI G_wGCw'C Cuts at: 0 989 1185 1413 Size: 989 196 228 Fragments arranged by size: 989 228 196 BsmI GAATG_Cn' Cuts at: 0 611 1413 Size: 611 802 Bsp1286I G_dGCh'C Cuts at: 0 347 379 727 989 1037 1185 1413 Size: 347 32 348 262 48 148 228 Fragments arranged by size: 348 347 262 228 148 48 32 BsrBI CCG'CTC Cuts at: 0 1178 1413 Size: 1178 235 BstAPI GCAn_nnn'nTGC Cuts at: 0 606 1047 1413 Size: 606 441 366 Fragments arranged by size: 606 441 366 BstBI TT'CG_AA Cuts at: 0 809 1413 Size: 809 604 Bsu36I CC'TnA_GG Cuts at: 0 677 692 1413 Size: 677 15 721 Fragments arranged by size: 721 677 15 BtgI C'CryG_G Cuts at: 0 395 1097 1413 Size: 395 702 316 Fragments arranged by size: 702 395 316 BtsI GCAGTG_nn' Cuts at: 0 632 1413 Size: 632 781 EaeI y'GGCC_r Cuts at: 0 1055 1413 Size: 1055 358 EarI CTCTTCn'nnn_ Cuts at: 0 88 367 463 913 1413 Size: 88 279 96 450 500 Fragments arranged by size: 500 450 279 96 88 EcoICRI GAG'CTC Cuts at: 0 987 1183 1413 Size: 987 196 230 Fragments arranged by size: 987 230 196 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 513 772 808 862 903 1013 1413 Size: 513 259 36 54 41 110 400 Fragments arranged by size: 513 400 259 110 54 41 36 EcoNI CCTnn'n_nnAGG Cuts at: 0 444 1413 Size: 444 969 EcoO109I rG'GnC_Cy Cuts at: 0 5 1413 Size: 5 1408 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 445 477 1137 1169 1413 Size: 445 32 660 32 244 Fragments arranged by size: 660 445 244 32 32 HincII GTy'rAC Cuts at: 0 632 1413 Size: 632 781 HindIII A'AGCT_T Cuts at: 0 1326 1413 Size: 1326 87 KpnI G_GTAC'C Cuts at: 0 1360 1413 Size: 1360 53 MscI TGG'CCA Cuts at: 0 1057 1413 Size: 1057 356 MslI CAynn'nnrTG Cuts at: 0 462 1413 Size: 462 951 MspA1I CmG'CkG Cuts at: 0 1047 1069 1413 Size: 1047 22 344 Fragments arranged by size: 1047 344 22 NcoI C'CATG_G Cuts at: 0 395 1413 Size: 395 1018 PflMI CCAn_nnn'nTGG Cuts at: 0 973 1413 Size: 973 440 PfoI T'CCnGG_A Cuts at: 0 529 911 1413 Size: 529 382 502 Fragments arranged by size: 529 502 382 PpuMI rG'GwC_Cy Cuts at: 0 5 1413 Size: 5 1408 PvuII CAG'CTG Cuts at: 0 1047 1413 Size: 1047 366 SacI G_AGCT'C Cuts at: 0 989 1185 1413 Size: 989 196 228 Fragments arranged by size: 989 228 196 SapI GCTCTTCn'nnn_ Cuts at: 0 367 463 1413 Size: 367 96 950 Fragments arranged by size: 950 367 96 SfcI C'TryA_G Cuts at: 0 166 1413 Size: 166 1247 StuI AGG'CCT Cuts at: 0 821 1413 Size: 821 592 StyI C'CwwG_G Cuts at: 0 36 275 395 998 1413 Size: 36 239 120 603 415 Fragments arranged by size: 603 415 239 120 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 213 429 1386 1413 Size: 213 216 957 27 Fragments arranged by size: 957 216 213 27 Tth111I GACn'n_nGTC Cuts at: 0 537 1257 1413 Size: 537 720 156 Fragments arranged by size: 720 537 156 XmnI GAAnn'nnTTC Cuts at: 0 1329 1413 Size: 1329 84 Enzymes that do cut: Acc65I AcuI AflIII AlwNI BanI BanII BbsI BglI Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BsrBI BstAPI BstBI Bsu36I BtgI BtsI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I Hin4I HincII HindIII KpnI MscI MslI MspA1I NcoI PflMI PfoI PpuMI PvuII SacI SapI SfcI StuI StyI TaqII Tth111I XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BpuEI BsaBI BsaXI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstEII BstXI BstYI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoRI EcoRV FalI FseI FspI FspAI HaeII HpaI KasI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI XbaI XcmI XhoI XmaI ZraI