################################## # NO CUT ENZYMES ( 204 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MfeI MflI MluI MluNI MroI MscI MseI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AccI 235 GTAGAC GT^[AC][GT]AC AlwI 27 GATCC GGATC(4/5) AocI 78 CCTCAGG CC^T[ACGT]AGG BbvCI 36 CCTCAGC CCTCAGC(-5/-2) BmyI 215 GGGCAC G[AGT]GC[ACT]^C BpmI 160 CTCCAG CTGGAG(16/14) BsaWI 122 ACCGGA [AT]^CCGG[AT] BseRI 206 GAGGAG GAGGAG(10/8) Bsp1286I 215 GGGCAC G[AGT]GC[ACT]^C BspHI 170 TCATGA T^CATGA Bsu36I 78 CCTCAGG CC^T[ACGT]AGG DpnII 27 GATC ^GATC Eco81I 78 CCTCAGG CC^T[ACGT]AGG FauI 149 CCCGC CCCGC(4/6) HapII 123 CCGG C^CGG HpaII 123 CCGG C^CGG MboI 27 GATC ^GATC MslI 279 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 139 CAGCTG C[AC]G^C[GT]G MspI 123 CCGG C^CGG NdeII 27 GATC ^GATC NspI 242 GCATGT [GA]CATG^[CT] PstI 295 CTGCAG CTGCA^G PvuII 139 CAGCTG CAG^CTG RcaI 170 TCATGA T^CATGA Sau3AI 27 GATC ^GATC SfcI 295 CTGCAG C^T[GA][CT]AG Tsp509I 269 AATT ^AATT TspRI 52 CACTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### BsaJI 314 CCCAGG C^C[ACGT][ACGT]GG 315 CCAGGG BstF5I 279 CATCC GGATG(2/0) 83 GGATG Cfr13I 135 GGGCC G^G[ACGT]CC 288 GGCCC DdeI 37 CTCAG C^T[ACGT]AG 79 CTCAG FokI 279 CATCC GGATG(9/13) 83 GGATG HaeIII 136 GGCC GG^CC 288 GGCC HphI 32 TCACC GGTGA(8/7) 110 TCACC MboII 17 TCTTC GAAGA(8/7) 107 TCTTC NlaIII 171 CATG CATG^ 243 CATG Sau96I 135 GGGCC G^G[ACGT]CC 288 GGCCC ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### AciI AluI BbvI BssKI BstNI Cac8I EcoRII Fnu4HI ItaI MnlI MvaI MwoI ScrFI SfaNI TseI