(Linear) (Six-base) MAPSORT of: KIEE6543.seq  Check: 200  from: 1  to: 321



KIEE6543.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AccI GT'mk_AC

Cuts at:      0     236     321 
   Size:        236      85
 
BbvCI CC'TCA_GC

Cuts at:      0      37     321 
   Size:         37     284
 
Bme1580I G_kGCm'C

Cuts at:      0     219     321 
   Size:        219     102
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     321 
   Size:        145     176
 
Bpu10I CC'TnA_GC

Cuts at:      0      37     321 
   Size:         37     284
 
BsaWI w'CCGG_w

Cuts at:      0     122     321 
   Size:        122     199
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     295     321 
   Size:        295      26
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     221     321 
   Size:        221     100
 
BseYI C'CCAG_C

Cuts at:      0     132     321 
   Size:        132     189
 
Bsp1286I G_dGCh'C

Cuts at:      0     219     321 
   Size:        219     102
 
BspHI T'CATG_A

Cuts at:      0     170     321 
   Size:        170     151
 
Bsu36I CC'TnA_GG

Cuts at:      0      79     321 
   Size:         79     242
 
BtsI GCAGTG_nn'

Cuts at:      0      51     321 
   Size:         51     270
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     135     321 
   Size:        135     186
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     321 
   Size:        145     176
 
MslI CAynn'nnrTG

Cuts at:      0     283     321 
   Size:        283      38
 
MspA1I CmG'CkG

Cuts at:      0     141     321 
   Size:        141     180
 
NspI r_CATG'y

Cuts at:      0     246     321 
   Size:        246      75
 
PasI CC'CwG_GG

Cuts at:      0     315     321 
   Size:        315       6
 
PfoI T'CCnGG_A

Cuts at:      0     161     321 
   Size:        161     160
 
PstI C_TGCA'G

Cuts at:      0     299     321 
   Size:        299      22
 
PvuII CAG'CTG

Cuts at:      0     141     321 
   Size:        141     180
 
SfcI C'TryA_G

Cuts at:      0     295     321 
   Size:        295      26
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     295     321 
   Size:        295      26
 

 Enzymes that do cut:

     AccI    BbvCI Bme1580I     BpmI   Bpu10I    BsaWI    BsaXI    BseRI
    BseYI Bsp1286I    BspHI   Bsu36I     BtsI     EciI  Eco57MI     MslI
   MspA1I     NspI     PasI     PfoI     PstI    PvuII     SfcI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI