Restriction map for
pFN21AE5440
(Circular) (Six-base) MAPSORT of: pFN21AE5440.seq Check: 427 from: 1 to: 5692
pFN21AE5440 5692 bp
With 182 enzymes: SgfI *
January 30, 2014 17:20 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3502 278
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2100 3035 1937
Size: 163 935 4594
Fragments arranged by size:
4594 935 163
Acc65I G'GTAC_C
Cuts at: 3013 3013
Size: 5692
AclI AA'CG_TT
Cuts at: 1563 3819 4192 1563
Size: 2256 373 3063
Fragments arranged by size:
3063 2256 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2183 2791 3755 5139 5407 1632
Size: 551 608 964 1384 268 1917
Fragments arranged by size:
1917 1384 964 608 551 268
AfeI AGC'GCT
Cuts at: 2948 2948
Size: 5692
AflII C'TTAA_G
Cuts at: 820 1017 2080 820
Size: 197 1063 4432
Fragments arranged by size:
4432 1063 197
AflIII A'CryG_T
Cuts at: 1203 2090 4591 5568 1203
Size: 887 2501 977 1327
Fragments arranged by size:
2501 1327 977 887
AgeI A'CCGG_T
Cuts at: 4523 4523
Size: 5692
AhdI GACnn_n'nnGTC
Cuts at: 2446 4421 2446
Size: 1975 3717
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5692
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5692
AlwNI CAG_nnn'CTG
Cuts at: 2920 3435 5007 2920
Size: 515 1572 3605
Fragments arranged by size:
3605 1572 515
ApaLI G'TGCA_C
Cuts at: 3749 4905 3749
Size: 1156 4536
ApoI r'AATT_y
Cuts at: 1493 2765 2781 3001 3227 3379 5488 1493
Size: 1272 16 220 226 152 2109 1697
Fragments arranged by size:
2109 1697 1272 226 220 152 16
AseI AT'TA_AT
Cuts at: 160 1032 4246 160
Size: 872 3214 1606
Fragments arranged by size:
3214 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
AvaI C'yCGr_G
Cuts at: 1870 1943 3017 1870
Size: 73 1074 4545
Fragments arranged by size:
4545 1074 73
BamHI G'GATC_C
Cuts at: 2371 3022 3389 2371
Size: 651 367 4674
Fragments arranged by size:
4674 651 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3013 4468 618
Size: 325 217 627 1226 1455 1842
Fragments arranged by size:
1842 1455 1226 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3011 1391
Size: 1620 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2123 2618 928
Size: 971 224 495 4002
Fragments arranged by size:
4002 971 495 224
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3883 3917 1903
Size: 34 1946 34 3678
Fragments arranged by size:
3678 1946 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2899 3584 4794 2899
Size: 685 1210 3797
Fragments arranged by size:
3797 1210 685
BclI T'GATC_A
Cuts at: 1548 2835 1548
Size: 1287 4405
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4303 136
Size: 107 122 71 631 605 2631 1525
Fragments arranged by size:
2631 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2351 5687 2351
Size: 3336 2356
BlpI GC'TnA_GC
Cuts at: 3109 3109
Size: 5692
Bme1580I G_kGCm'C
Cuts at: 1790 3753 4909 1790
Size: 1963 1156 2573
Fragments arranged by size:
2573 1963 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5692
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3423 4381 442
Size: 416 53 83 399 24 2006 958 1753
Fragments arranged by size:
2006 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5692
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4352 1239
Size: 3113 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2745 3817 4682 4980 2745
Size: 1072 865 298 3457
Fragments arranged by size:
3457 1072 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4355 882
Size: 617 2856 2219
Fragments arranged by size:
2856 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2091 3483 493
Size: 711 887 1392 2702
Fragments arranged by size:
2702 1392 887 711
BsaBI GATnn'nnATC
Cuts at: 5686 5686
Size: 5692
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3499 3881 4516 275
Size: 53 83 186 936 1966 382 635 1451
Fragments arranged by size:
1966 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4125 4523 4797 4944 5355 4125
Size: 398 274 147 411 4462
Fragments arranged by size:
4462 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2042 2072 2874 2904 1178
Size: 30 165 30 639 30 802 30 3966
Fragments arranged by size:
3966 802 639 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2597 1186
Size: 1411 4281
BseYI C'CCAG_C
Cuts at: 4895 5391 4895
Size: 496 5196
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2529 2580 1163
Size: 573 793 51 4275
Fragments arranged by size:
4275 793 573 51
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3142 3903 4931 1540
Size: 275 186 1141 761 1028 2301
Fragments arranged by size:
2301 1141 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3753 3838 4909 3753
Size: 85 1071 4536
Fragments arranged by size:
4536 1071 85
BsmI GAATG_Cn'
Cuts at: 3203 3296 3203
Size: 93 5599
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3011 3753 3838 4909 1391
Size: 399 1221 742 85 1071 2174
Fragments arranged by size:
2174 1221 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5355 5355
Size: 5692
BspHI T'CATG_A
Cuts at: 3581 5311 3581
Size: 1730 3962
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2400 3047 844
Size: 1556 647 3489
Fragments arranged by size:
3489 1556 647
BsrBI CCG'CTC
Cuts at: 1394 1823 2974 3579 1394
Size: 429 1151 605 3507
Fragments arranged by size:
3507 1151 605 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4187 4361 65
Size: 1183 2939 174 1396
Fragments arranged by size:
2939 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2567 4336 4523 1700
Size: 110 757 1769 187 2869
Fragments arranged by size:
2869 1769 757 187 110
BsrGI T'GTAC_A
Cuts at: 96 2712 96
Size: 2616 3076
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5692
BssSI C'ACGA_G
Cuts at: 3752 4764 3752
Size: 1012 4680
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2679 794
Size: 1885 3807
BstBI TT'CG_AA
Cuts at: 4496 4496
Size: 5692
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5692
BstXI CCAn_nnnn'nTGG
Cuts at: 2728 2728
Size: 5692
BstYI r'GATC_y
Cuts at: 1972 2351 2371 2479 3022 3389 3774 3791 5243
Size: 379 20 108 543 367 385 17 1452
Cuts at: 5243 5329 5341 5687 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 543 385 379 367 346 108
86 20 17 12
Bsu36I CC'TnA_GG
Cuts at: 2367 2847 2367
Size: 480 5212
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5140
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4953
Fragments arranged by size:
4953 683 56
BtsI GCAGTG_nn'
Cuts at: 2592 2895 3208 4002 4022 2592
Size: 303 313 794 20 4262
Fragments arranged by size:
4262 794 313 303 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5692
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5657
DraI TTT'AAA
Cuts at: 2996 2996
Size: 5692
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4699 809
Size: 507 3383 1802
Fragments arranged by size:
3383 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2397 2488 2565
Size: 54 1425 50 275 18 567 91 77
Cuts at: 2565 3139 4028 5657 8
Size: 574 889 1629 43
Fragments arranged by size:
1629 1425 889 574 567 275 91 77
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3139 1537
Size: 275 1327 4090
Fragments arranged by size:
4090 1327 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3622 1353
Size: 2269 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4279 4653 4799 387
Size: 1064 138 2690 374 146 1280
Fragments arranged by size:
2690 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 2791 3755 4352 5139 5407 1239
Size: 393 551 608 964 597 787 268 1524
Fragments arranged by size:
1524 964 787 608 597 551 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2587 2587
Size: 5692
EcoRI G'AATT_C
Cuts at: 2781 3001 3379 2781
Size: 220 378 5094
Fragments arranged by size:
5094 378 220
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2498 2498
Size: 5692
FspI TGC'GCA
Cuts at: 4198 4198
Size: 5692
HaeII r_GCGC'y
Cuts at: 1678 2950 4839 1678
Size: 1272 1889 2531
Fragments arranged by size:
2531 1889 1272
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2132 2164 2209 2241 2432
Size: 32 135 32 560 32 45 32 191
Cuts at: 2432 2464 2525 2557 4333 4365 4407 4439 1373
Size: 32 61 32 1776 32 42 32 2626
Fragments arranged by size:
2626 1776 560 191 135 61 45 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3036 3282 669
Size: 988 281 1098 246 3079
Fragments arranged by size:
3079 1098 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5692
HpaI GTT'AAC
Cuts at: 3282 3282
Size: 5692
KpnI G_GTAC'C
Cuts at: 3017 3017
Size: 5692
MfeI C'AATT_G
Cuts at: 3291 3291
Size: 5692
MluI A'CGCG_T
Cuts at: 5568 5568
Size: 5692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2302 4806 4990 1305
Size: 997 2504 184 2007
Fragments arranged by size:
2504 2007 997 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2399 2490 10
Size: 54 1425 343 567 91 3212
Fragments arranged by size:
3212 1425 567 343 91 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2726 3650 4009 4168 518
Size: 648 1560 924 359 159 2042
Fragments arranged by size:
2042 1560 924 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3108 3785 4933 5178 1606
Size: 1502 677 1148 245 2120
Fragments arranged by size:
2120 1502 1148 677 245
NaeI GCC'GGC
Cuts at: 2569 2569
Size: 5692
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5140
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5692
NgoMIV G'CCGG_C
Cuts at: 2567 2567
Size: 5692
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5692
NotI GC'GGCC_GC
Cuts at: 3139 3139
Size: 5692
NspI r_CATG'y
Cuts at: 1132 1229 2965 3050 4595 1132
Size: 97 1736 85 1545 2229
Fragments arranged by size:
2229 1736 1545 97 85
PasI CC'CwG_GG
Cuts at: 1348 2644 1348
Size: 1296 4396
PciI A'CATG_T
Cuts at: 4591 4591
Size: 5692
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5692
PfoI T'CCnGG_A
Cuts at: 2174 2606 2174
Size: 432 5260
PmeI GTTT'AAAC
Cuts at: 2996 2996
Size: 5692
PmlI CAC'GTG
Cuts at: 2091 2091
Size: 5692
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3761 3793 5274 5306 3761
Size: 32 1481 32 4147
Fragments arranged by size:
4147 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5692
PsiI TTA'TAA
Cuts at: 3262 3262
Size: 5692
PstI C_TGCA'G
Cuts at: 830 2280 2775 3044 830
Size: 1450 495 269 3478
Fragments arranged by size:
3478 1450 495 269
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5692
SalI G'TCGA_C
Cuts at: 1936 3034 1936
Size: 1098 4594
SbfI CC_TGCA'GG
Cuts at: 3044 3044
Size: 5692
ScaI AGT'ACT
Cuts at: 3940 5652 3940
Size: 1712 3980
SfcI C'TryA_G
Cuts at: 826 1045 2276 2771 3040 4175 4856 5047 826
Size: 219 1231 495 269 1135 681 191 1471
Fragments arranged by size:
1471 1231 1135 681 495 269 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5692
SmaI CCC'GGG
Cuts at: 1872 3019 1872
Size: 1147 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2080 2760 3796 4697 4959 820
Size: 197 926 137 680 1036 901 262 1553
Fragments arranged by size:
1553 1036 926 901 680 262 197 137
SnaBI TAC'GTA
Cuts at: 493 3483 493
Size: 2990 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5692
SphI G_CATG'C
Cuts at: 1132 3050 1132
Size: 1918 3774
SspI AAT'ATT
Cuts at: 5 52 3616 5
Size: 47 3564 2081
Fragments arranged by size:
3564 2081 47
StuI AGG'CCT
Cuts at: 1517 2845 1517
Size: 1328 4364
StyI C'CwwG_G
Cuts at: 513 1065 3131 513
Size: 552 2066 3074
Fragments arranged by size:
3074 2066 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2457 3719 3736 3889 4074 4519 325
Size: 1255 877 1262 17 153 185 445 1498
Fragments arranged by size:
1498 1262 1255 877 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2712 3938 5650
Size: 274 80 33 51 1443 735 1226 1712
Cuts at: 5650 96
Size: 138
Fragments arranged by size:
1712 1443 1226 735 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2042 2074 2874 2906 5652 5684 2042
Size: 32 800 32 2746 32 2050
Fragments arranged by size:
2746 2050 800 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5692
XbaI T'CTAG_A
Cuts at: 3028 3028
Size: 5692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2728 2728
Size: 5692
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5692
XmaI C'CCGG_G
Cuts at: 1870 3017 1870
Size: 1147 4545
XmnI GAAnn'nnTTC
Cuts at: 3821 3821
Size: 5692
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3500 276
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI