################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII AccI AclI AcsI AflII AgeI AhdI ApoI AscI AseI AsnI Asp700I AspEI AvrII BbrPI BbvCI BciVI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsiWI BspDI BspHI BsrBI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraI Eam1105I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI HincII HindII HindIII HpaI MaeII MamI MfeI MluI MseI MunI NaeI NcoI NdeI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SalI SbfI ScaI SfcI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI StyI SwaI Tru9I Tsp509I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 953 GGTACC G^GTACC AccIII 481 TCCGGA T^CCGGA AflIII 473 ACATGT A^C[GA][CT]GT AocI 1042 CCTGAGG CC^T[ACGT]AGG ApaI 41 GGGCCC GGGCC^C Asp718I 953 GGTACC G^GTACC AvaII 125 GGTCC G^G[AT]CC AviII 386 TGCGCA TGC^GCA BalI 234 TGGCCA TGG^CCA BamHI 732 GGATCC G^GATCC BanII 41 GGGCCC G[GA]GC[CT]^C BbsI 1358 GTCTTC GAAGAC(2/6) BclI 998 TGATCA T^GATCA BfaI 79 CTAG C^TAG BglII 461 AGATCT A^GATCT BpmI 753 CTCCAG CTGGAG(16/14) BpuAI 1358 GTCTTC GAAGAC(2/6) BsaWI 481 TCCGGA [AT]^CCGG[AT] BseAI 481 TCCGGA T^CCGGA BsiEI 402 CGGCCG CG[GA][CT]^CG BsmBI 855 CGTCTC CGTCTC(1/5) BspEI 481 TCCGGA T^CCGGA BspLU11I 473 ACATGT A^CATGT BsrDI 84 CATTGC GCAATG(2/0) BsrFI 195 GCCGGT [GA]^CCGG[CT] BssSI 661 CACGAG CACGAG(-5/-1) Bsu36I 1042 CCTGAGG CC^T[ACGT]AGG Cfr10I 195 GCCGGT [GA]^CCGG[CT] DrdI 73 GACATCCTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 402 CGGCCG C^GGCCG EclXI 402 CGGCCG C^GGCCG Eco52I 402 CGGCCG C^GGCCG Eco81I 1042 CCTGAGG CC^T[ACGT]AGG FbaI 998 TGATCA T^GATCA FspI 386 TGCGCA TGC^GCA KpnI 953 GGTACC GGTAC^C KspI 1099 CCGCGG CCGC^GG MaeI 79 CTAG C^TAG MluNI 234 TGGCCA TGG^CCA MroI 481 TCCGGA T^CCGGA MscI 234 TGGCCA TGG^CCA NotI 401 GCGGCCGC GC^GGCCGC NspI 473 ACATGT [GA]CATG^[CT] PshAI 612 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 1207 CAGCTG CAG^CTG SacII 1099 CCGCGG CCGC^GG SexAI 881 ACCAGGT A^CC[AT]GGT SfiI 42 GGCCCAGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### Alw44I 1250 GTGCAC G^TGCAC 1318 GTGCAC Aor51HI 392 AGCGCT AGC^GCT 896 AGCGCT ApaLI 1250 GTGCAC G^TGCAC 1318 GTGCAC AspI 720 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 880 GACCAGGTC BbeI 631 GGCGCC GGCGC^C 1130 GGCGCC BglI 43 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1122 GCCCAACGGGC BsaI 682 GGTCTC GGTCTC(1/5) 1309 GGTCTC BsmFI 256 GGGAC GGGAC(10/14) 1141 GGGAC BsmI 300 GAATGC GAATGC(1/-1) 1007 GCATTC Bsp1407I 446 TGTACA T^GTACA 593 TGTACA BspMI 383 ACCTGC ACCTGC(4/8) 791 ACCTGC BsrGI 446 TGTACA T^GTACA 593 TGTACA DdeI 465 CTCAG C^T[ACGT]AG 1043 CTGAG Eco47III 392 AGCGCT AGC^GCT 896 AGCGCT HgaI 176 GACGC GACGC(5/10) 966 GACGC KasI 631 GGCGCC G^GCGCC 1130 GGCGCC NarI 631 GGCGCC GG^CGCC 1130 GGCGCC PflFI 720 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 880 GACCAGGTC SapI 912 GAAGAGC GCTCTTC(1/4) 306 GCTCTTC SfoI 631 GGCGCC GGC^GCC 1130 GGCGCC SspBI 446 TGTACA T^GTACA 593 TGTACA TfiI 290 GATTC G^A[AT]TC 1180 GATTC Tsp45I 568 GTGAC ^GT[GC]AC 808 GTGAC Tth111I 720 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 880 GACCAGGTC XcmI 458 CCAAGATCTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 971 CCATGTTTCTCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaI BanI BbvI BcgI BcnI BmyI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DpnII DraII DraIII DsaI EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TseI TspRI TthHB8I Van91I XhoII