(Circular) (Six-base) MAPSORT of: pFN21AE5578.seq Check: 2808 from: 1 to: 6079 pFN21AE5578 6079 bp With 182 enzymes: SgfI * January 30, 2014 17:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2805 2805 Size: 6079 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3889 278 Size: 53 83 186 936 2353 2468 Fragments arranged by size: 2468 2353 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3422 1937 Size: 1485 4594 Acc65I G'GTAC_C Cuts at: 2958 3400 2958 Size: 442 5637 AclI AA'CG_TT Cuts at: 1563 4206 4579 1563 Size: 2643 373 3063 Fragments arranged by size: 3063 2643 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2984 3350 4142 5526 5794 1632 Size: 1352 366 792 1384 268 1917 Fragments arranged by size: 1917 1384 1352 792 366 268 AfeI AGC'GCT Cuts at: 2399 2903 2399 Size: 504 5575 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5882 AflIII A'CryG_T Cuts at: 1203 2478 4978 5955 1203 Size: 1275 2500 977 1327 Fragments arranged by size: 2500 1327 1275 977 AgeI A'CCGG_T Cuts at: 4910 4910 Size: 6079 AhdI GACnn_n'nnGTC Cuts at: 4808 4808 Size: 6079 AleI CACnn'nnGTG Cuts at: 1166 3077 1166 Size: 1911 4168 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3234 1184 Size: 2050 4029 AlwNI CAG_nnn'CTG Cuts at: 2264 2389 2512 2766 3822 5394 2264 Size: 125 123 254 1056 1572 2949 Fragments arranged by size: 2949 1572 1056 254 125 123 ApaI G_GGCC'C Cuts at: 2050 2050 Size: 6079 ApaLI G'TGCA_C Cuts at: 3255 3323 4136 5292 3255 Size: 68 813 1156 4042 Fragments arranged by size: 4042 1156 813 68 ApoI r'AATT_y Cuts at: 1493 3388 3614 3766 5875 1493 Size: 1895 226 152 2109 1697 Fragments arranged by size: 2109 1895 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4633 160 Size: 872 3601 1606 Fragments arranged by size: 3601 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6079 AvaI C'yCGr_G Cuts at: 1870 1943 2863 3189 3206 3404 1870 Size: 73 920 326 17 198 4545 Fragments arranged by size: 4545 920 326 198 73 17 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2336 2369 2336 Size: 33 6046 BamHI G'GATC_C Cuts at: 2737 3409 3776 2737 Size: 672 367 5040 Fragments arranged by size: 5040 672 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2032 2636 2805 2958 3043 Size: 325 217 627 245 604 169 153 85 Cuts at: 3043 3135 3400 4855 618 Size: 92 265 1455 1842 Fragments arranged by size: 1842 1455 627 604 325 265 245 217 169 153 92 85 BanII G_rGCy'C Cuts at: 1391 2050 3398 1391 Size: 659 1348 4072 Fragments arranged by size: 4072 1348 659 BbeI G_GCGC'C Cuts at: 2640 3139 2640 Size: 499 5580 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3356 928 Size: 971 1457 3651 Fragments arranged by size: 3651 1457 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2288 2322 2383 2417 2700 2734 4270 Size: 34 351 34 61 34 283 34 1536 Cuts at: 4270 4304 1903 Size: 34 3678 Fragments arranged by size: 3678 1536 351 283 61 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3971 5181 3971 Size: 1210 4869 BclI T'GATC_A Cuts at: 1548 3003 1548 Size: 1455 4624 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2054 3133 4690 Size: 107 122 71 631 605 382 1079 1557 Cuts at: 4690 136 Size: 1525 Fragments arranged by size: 1557 1525 1079 631 605 382 122 107 71 BglII A'GATC_T Cuts at: 2466 6074 2466 Size: 3608 2471 BlpI GC'TnA_GC Cuts at: 3496 3496 Size: 6079 Bme1580I G_kGCm'C Cuts at: 1790 2050 3237 3259 3327 4140 5296 1790 Size: 260 1187 22 68 813 1156 2573 Fragments arranged by size: 2573 1187 1156 813 260 68 22 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6079 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2339 3810 4768 Size: 416 53 83 399 24 922 1471 958 Cuts at: 4768 442 Size: 1753 Fragments arranged by size: 1753 1471 958 922 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6079 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2044 2044 Size: 6079 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2743 4739 1239 Size: 1504 1996 2579 Fragments arranged by size: 2579 1996 1504 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4204 5069 5367 4204 Size: 865 298 4916 Fragments arranged by size: 4916 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2693 3320 4742 882 Size: 617 1194 627 1422 2219 Fragments arranged by size: 2219 1422 1194 627 617 BsaAI yAC'GTr Cuts at: 493 1204 3870 493 Size: 711 2666 2702 Fragments arranged by size: 2702 2666 711 BsaBI GATnn'nnATC Cuts at: 6073 6073 Size: 6079 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2637 2972 3136 3886 Size: 53 83 186 936 1104 335 164 750 Cuts at: 3886 4268 4903 275 Size: 382 635 1451 Fragments arranged by size: 1451 1104 936 750 635 382 335 186 164 83 53 BsaWI w'CCGG_w Cuts at: 2486 4512 4910 5184 5331 5742 2486 Size: 2026 398 274 147 411 2823 Fragments arranged by size: 2823 2026 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2468 2498 1178 Size: 30 165 30 1065 30 4759 Fragments arranged by size: 4759 1065 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2834 3291 1186 Size: 838 810 457 3974 Fragments arranged by size: 3974 838 810 457 BseYI C'CCAG_C Cuts at: 3236 5282 5778 3236 Size: 2046 496 3537 Fragments arranged by size: 3537 2046 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2158 2899 2918 3076 3240 1163 Size: 573 422 741 19 158 164 4002 Fragments arranged by size: 4002 741 573 422 164 158 19 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2410 3529 4290 5318 1540 Size: 275 186 409 1119 761 1028 2301 Fragments arranged by size: 2301 1119 1028 761 409 275 186 BsiHKAI G_wGCw'C Cuts at: 2016 2439 3259 3327 4140 4225 5296 2016 Size: 423 820 68 813 85 1071 2799 Fragments arranged by size: 2799 1071 820 813 423 85 68 BsmI GAATG_Cn' Cuts at: 2311 3012 3590 3683 2311 Size: 701 578 93 4707 Fragments arranged by size: 4707 701 578 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2866 2866 Size: 6079 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2050 2439 3237 3259 3327 3398 Size: 399 226 34 389 798 22 68 71 Cuts at: 3398 4140 4225 5296 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 798 742 399 389 226 85 71 68 34 22 BspEI T'CCGG_A Cuts at: 2486 5742 2486 Size: 3256 2823 BspHI T'CATG_A Cuts at: 3968 5698 3968 Size: 1730 4349 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2397 2805 3434 844 Size: 1553 408 629 3489 Fragments arranged by size: 3489 1553 629 408 BsrBI CCG'CTC Cuts at: 1394 1823 3966 1394 Size: 429 2143 3507 Fragments arranged by size: 3507 2143 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2088 4574 4748 65 Size: 1183 840 2486 174 1396 Fragments arranged by size: 2486 1396 1183 840 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2200 4723 4910 1700 Size: 110 390 2523 187 2869 Fragments arranged by size: 2869 2523 390 187 110 BsrGI T'GTAC_A Cuts at: 96 2451 2598 96 Size: 2355 147 3577 Fragments arranged by size: 3577 2355 147 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6079 BssSI C'ACGA_G Cuts at: 2666 4139 5151 2666 Size: 1473 1012 3594 Fragments arranged by size: 3594 1473 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6079 BstBI TT'CG_AA Cuts at: 4883 4883 Size: 6079 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6079 BstYI r'GATC_y Cuts at: 1972 2466 2737 2821 3409 3776 4161 4178 5630 Size: 494 271 84 588 367 385 17 1452 Cuts at: 5630 5716 5728 6074 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 588 494 385 367 346 271 86 84 17 12 Bsu36I CC'TnA_GG Cuts at: 3048 3048 Size: 6079 BtgI C'CryG_G Cuts at: 513 1065 2004 2745 2828 3104 513 Size: 552 939 741 83 276 3488 Fragments arranged by size: 3488 939 741 552 276 83 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2524 3079 487 Size: 683 56 1298 555 3487 Fragments arranged by size: 3487 1298 683 555 56 BtsI GCAGTG_nn' Cuts at: 2242 3595 4389 4409 2242 Size: 1353 794 20 3912 Fragments arranged by size: 3912 1353 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6079 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6044 DraI TTT'AAA Cuts at: 3383 3383 Size: 6079 DraIII CAC_nnn'GTG Cuts at: 2248 2671 2812 2248 Size: 423 141 5515 Fragments arranged by size: 5515 423 141 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2084 5086 809 Size: 507 768 3002 1802 Fragments arranged by size: 3002 1802 768 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2198 2239 2407 Size: 54 1425 50 275 18 368 41 168 Cuts at: 2407 2586 2910 3526 4415 6044 8 Size: 179 324 616 889 1629 43 Fragments arranged by size: 1629 1425 889 616 368 324 275 179 168 54 50 43 41 18 EagI C'GGCC_G Cuts at: 1537 1812 2407 3526 1537 Size: 275 595 1119 4090 Fragments arranged by size: 4090 1119 595 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2222 2318 2696 2912 4009 1353 Size: 869 96 378 216 1097 3423 Fragments arranged by size: 3423 1097 869 378 216 96 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2408 4666 5040 5186 387 Size: 1064 138 819 2258 374 146 1280 Fragments arranged by size: 2258 1280 1064 819 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2743 2984 3350 4142 4739 5526 5794 Size: 393 1111 241 366 792 597 787 268 Cuts at: 5794 1239 Size: 1524 Fragments arranged by size: 1524 1111 792 787 597 393 366 268 241 EcoO109I rG'GnC_Cy Cuts at: 2046 2056 2208 3158 2046 Size: 10 152 950 4967 Fragments arranged by size: 4967 950 152 10 EcoRI G'AATT_C Cuts at: 3388 3766 3388 Size: 378 5701 FspI TGC'GCA Cuts at: 2393 4585 2393 Size: 2192 3887 HaeII r_GCGC'y Cuts at: 1678 2271 2401 2640 2905 3139 5226 1678 Size: 593 130 239 265 234 2087 2531 Fragments arranged by size: 2531 2087 593 265 239 234 130 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2053 2085 2468 2500 2806 Size: 32 135 32 481 32 383 32 306 Cuts at: 2806 2838 2841 2873 4720 4752 4794 4826 1373 Size: 32 3 32 1847 32 42 32 2626 Fragments arranged by size: 2626 1847 481 383 306 135 42 32 32 32 32 32 32 32 32 3 HincII GTy'rAC Cuts at: 669 1657 1938 3423 3669 669 Size: 988 281 1485 246 3079 Fragments arranged by size: 3079 1485 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6079 HpaI GTT'AAC Cuts at: 3669 3669 Size: 6079 KasI G'GCGC_C Cuts at: 2636 3135 2636 Size: 499 5580 KpnI G_GTAC'C Cuts at: 2962 3404 2962 Size: 442 5637 MfeI C'AATT_G Cuts at: 3678 3678 Size: 6079 MluI A'CGCG_T Cuts at: 5955 5955 Size: 6079 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3114 5193 5377 1305 Size: 1809 2079 184 2007 Fragments arranged by size: 2079 2007 1809 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2241 10 Size: 54 1425 343 409 3848 Fragments arranged by size: 3848 1425 409 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2375 2558 2648 2990 3077 4037 4396 Size: 648 1209 183 90 342 87 960 359 Cuts at: 4396 4555 518 Size: 159 2042 Fragments arranged by size: 2042 1209 960 648 359 342 183 159 90 87 MspA1I CmG'CkG Cuts at: 1606 2363 3106 3214 3495 4172 5320 5565 1606 Size: 757 743 108 281 677 1148 245 2120 Fragments arranged by size: 2120 1148 757 743 677 281 245 108 NarI GG'CG_CC Cuts at: 2637 3136 2637 Size: 499 5580 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4588 Fragments arranged by size: 4588 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6079 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6079 NotI GC'GGCC_GC Cuts at: 2407 3526 2407 Size: 1119 4960 NspI r_CATG'y Cuts at: 1132 1229 2482 3437 4982 1132 Size: 97 1253 955 1545 2229 Fragments arranged by size: 2229 1545 1253 955 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6079 PciI A'CATG_T Cuts at: 2478 4978 2478 Size: 2500 3579 PflMI CCAn_nnn'nTGG Cuts at: 1108 2248 2671 3359 1108 Size: 1140 423 688 3828 Fragments arranged by size: 3828 1140 688 423 PmeI GTTT'AAAC Cuts at: 3383 3383 Size: 6079 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4148 4180 5661 5693 4148 Size: 32 1481 32 4534 Fragments arranged by size: 4534 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2621 1613 Size: 1008 5071 PsiI TTA'TAA Cuts at: 3649 3649 Size: 6079 PspOMI G'GGCC_C Cuts at: 2046 2046 Size: 6079 PstI C_TGCA'G Cuts at: 830 3431 830 Size: 2601 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6079 PvuII CAG'CTG Cuts at: 3214 3214 Size: 6079 SacII CC_GC'GG Cuts at: 3107 3107 Size: 6079 SalI G'TCGA_C Cuts at: 1936 3421 1936 Size: 1485 4594 SapI GCTCTTCn'nnn_ Cuts at: 2318 2912 2318 Size: 594 5485 SbfI CC_TGCA'GG Cuts at: 3431 3431 Size: 6079 ScaI AGT'ACT Cuts at: 4327 6039 4327 Size: 1712 4367 SexAI A'CCwGG_T Cuts at: 2886 2886 Size: 6079 SfcI C'TryA_G Cuts at: 826 1045 3427 4562 5243 5434 826 Size: 219 2382 1135 681 191 1471 Fragments arranged by size: 2382 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2054 2054 Size: 6079 SfoI GGC'GCC Cuts at: 2638 3137 2638 Size: 499 5580 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6079 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6079 SmaI CCC'GGG Cuts at: 1872 3406 1872 Size: 1534 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4183 5084 5346 820 Size: 197 926 2240 901 262 1553 Fragments arranged by size: 2240 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3870 493 Size: 3377 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6079 SphI G_CATG'C Cuts at: 1132 3437 1132 Size: 2305 3774 SspI AAT'ATT Cuts at: 5 52 4003 5 Size: 47 3951 2081 Fragments arranged by size: 3951 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6079 StyI C'CwwG_G Cuts at: 513 1065 2004 3518 513 Size: 552 939 1514 3074 Fragments arranged by size: 3074 1514 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4106 4123 4276 4461 4906 325 Size: 1255 2526 17 153 185 445 1498 Fragments arranged by size: 2526 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2451 2598 4325 Size: 274 80 33 51 1443 474 147 1727 Cuts at: 4325 6037 96 Size: 1712 138 Fragments arranged by size: 1727 1712 1443 474 274 147 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2813 2845 6039 6071 2813 Size: 32 3194 32 2821 Fragments arranged by size: 3194 2821 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2728 2888 1316 Size: 1412 160 4507 Fragments arranged by size: 4507 1412 160 XbaI T'CTAG_A Cuts at: 3415 3415 Size: 6079 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2470 2983 2470 Size: 513 5566 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6079 XmaI C'CCGG_G Cuts at: 1870 3404 1870 Size: 1534 4545 XmnI GAAnn'nnTTC Cuts at: 4208 4208 Size: 6079 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3887 276 Size: 53 83 186 936 2353 2468 Fragments arranged by size: 2468 2353 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SrfI SwaI