(Linear) (Six-base) MAPSORT of: KIEE5578.seq Check: 5336 from: 1 to: 1374 KIEE5578.orf With 182 enzymes: SgfI * January 29, 2014 12:06 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 800 1374 Size: 800 574 Acc65I G'GTAC_C Cuts at: 0 953 1374 Size: 953 421 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 979 1345 1374 Size: 979 366 29 Fragments arranged by size: 979 366 29 AfeI AGC'GCT Cuts at: 0 394 898 1374 Size: 394 504 476 Fragments arranged by size: 504 476 394 AflIII A'CryG_T Cuts at: 0 473 1374 Size: 473 901 AleI CACnn'nnGTG Cuts at: 0 1072 1374 Size: 1072 302 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1229 1374 Size: 1229 145 AlwNI CAG_nnn'CTG Cuts at: 0 259 384 507 761 1374 Size: 259 125 123 254 613 Fragments arranged by size: 613 259 254 125 123 ApaI G_GGCC'C Cuts at: 0 45 1374 Size: 45 1329 ApaLI G'TGCA_C Cuts at: 0 1250 1318 1374 Size: 1250 68 56 Fragments arranged by size: 1250 68 56 AvaI C'yCGr_G Cuts at: 0 858 1184 1201 1374 Size: 858 326 17 173 Fragments arranged by size: 858 326 173 17 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 331 364 1374 Size: 331 33 1010 Fragments arranged by size: 1010 331 33 BamHI G'GATC_C Cuts at: 0 732 1374 Size: 732 642 BanI G'GyrC_C Cuts at: 0 27 631 800 953 1038 1130 1374 Size: 27 604 169 153 85 92 244 Fragments arranged by size: 604 244 169 153 92 85 27 BanII G_rGCy'C Cuts at: 0 45 1374 Size: 45 1329 BbeI G_GCGC'C Cuts at: 0 635 1134 1374 Size: 635 499 240 Fragments arranged by size: 635 499 240 BbsI GAAGACnn'nnnn_ Cuts at: 0 1351 1374 Size: 1351 23 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 283 317 378 412 695 729 1374 Size: 283 34 61 34 283 34 645 Fragments arranged by size: 645 283 283 61 34 34 34 BclI T'GATC_A Cuts at: 0 998 1374 Size: 998 376 BglI GCCn_nnn'nGGC Cuts at: 0 49 1128 1374 Size: 49 1079 246 Fragments arranged by size: 1079 246 49 BglII A'GATC_T Cuts at: 0 461 1374 Size: 461 913 Bme1580I G_kGCm'C Cuts at: 0 45 1232 1254 1322 1374 Size: 45 1187 22 68 52 Fragments arranged by size: 1187 68 52 45 22 BmrI ACTGGGnnnn_n' Cuts at: 0 334 1374 Size: 334 1040 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 39 1374 Size: 39 1335 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 738 1374 Size: 738 636 BsaI GGTCTCn'nnnn_ Cuts at: 0 688 1315 1374 Size: 688 627 59 Fragments arranged by size: 688 627 59 BsaHI Gr'CG_yC Cuts at: 0 632 967 1131 1374 Size: 632 335 164 243 Fragments arranged by size: 632 335 243 164 BsaWI w'CCGG_w Cuts at: 0 481 1374 Size: 481 893 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 463 493 1374 Size: 463 30 881 Fragments arranged by size: 881 463 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 19 829 1286 1374 Size: 19 810 457 88 Fragments arranged by size: 810 457 88 19 BseYI C'CCAG_C Cuts at: 0 1231 1374 Size: 1231 143 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 153 894 913 1071 1235 1374 Size: 153 741 19 158 164 139 Fragments arranged by size: 741 164 158 153 139 19 BsiEI CG_ry'CG Cuts at: 0 405 1374 Size: 405 969 BsiHKAI G_wGCw'C Cuts at: 0 11 434 1254 1322 1374 Size: 11 423 820 68 52 Fragments arranged by size: 820 423 68 52 11 BsmI GAATG_Cn' Cuts at: 0 306 1007 1374 Size: 306 701 367 Fragments arranged by size: 701 367 306 BsmBI CGTCTCn'nnnn_ Cuts at: 0 861 1374 Size: 861 513 Bsp1286I G_dGCh'C Cuts at: 0 11 45 434 1232 1254 1322 1374 Size: 11 34 389 798 22 68 52 Fragments arranged by size: 798 389 68 52 34 22 11 BspEI T'CCGG_A Cuts at: 0 481 1374 Size: 481 893 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 392 800 1374 Size: 392 408 574 Fragments arranged by size: 574 408 392 BsrDI GCAATG_nn' Cuts at: 0 83 1374 Size: 83 1291 BsrFI r'CCGG_y Cuts at: 0 195 1374 Size: 195 1179 BsrGI T'GTAC_A Cuts at: 0 446 593 1374 Size: 446 147 781 Fragments arranged by size: 781 446 147 BssSI C'ACGA_G Cuts at: 0 661 1374 Size: 661 713 BstYI r'GATC_y Cuts at: 0 461 732 816 1374 Size: 461 271 84 558 Fragments arranged by size: 558 461 271 84 Bsu36I CC'TnA_GG Cuts at: 0 1043 1374 Size: 1043 331 BtgI C'CryG_G Cuts at: 0 740 823 1099 1374 Size: 740 83 276 275 Fragments arranged by size: 740 276 275 83 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 519 1074 1374 Size: 519 555 300 Fragments arranged by size: 555 519 300 BtsI GCAGTG_nn' Cuts at: 0 237 1374 Size: 237 1137 DraIII CAC_nnn'GTG Cuts at: 0 243 666 807 1374 Size: 243 423 141 567 Fragments arranged by size: 567 423 243 141 DrdI GACnn_nn'nnGTC Cuts at: 0 79 1374 Size: 79 1295 EaeI y'GGCC_r Cuts at: 0 193 234 402 581 905 1374 Size: 193 41 168 179 324 469 Fragments arranged by size: 469 324 193 179 168 41 EagI C'GGCC_G Cuts at: 0 402 1374 Size: 402 972 EarI CTCTTCn'nnn_ Cuts at: 0 217 313 691 907 1374 Size: 217 96 378 216 467 Fragments arranged by size: 467 378 217 216 96 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 403 1374 Size: 403 971 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 738 979 1345 1374 Size: 738 241 366 29 Fragments arranged by size: 738 366 241 29 EcoO109I rG'GnC_Cy Cuts at: 0 41 51 203 1153 1374 Size: 41 10 152 950 221 Fragments arranged by size: 950 221 152 41 10 FspI TGC'GCA Cuts at: 0 388 1374 Size: 388 986 HaeII r_GCGC'y Cuts at: 0 266 396 635 900 1134 1374 Size: 266 130 239 265 234 240 Fragments arranged by size: 266 265 240 239 234 130 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 48 80 463 495 801 833 836 868 Size: 48 32 383 32 306 32 3 32 Cuts at: 868 1374 Size: 506 Fragments arranged by size: 506 383 306 48 32 32 32 32 3 KasI G'GCGC_C Cuts at: 0 631 1130 1374 Size: 631 499 244 Fragments arranged by size: 631 499 244 KpnI G_GTAC'C Cuts at: 0 957 1374 Size: 957 417 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1109 1374 Size: 1109 265 MscI TGG'CCA Cuts at: 0 236 1374 Size: 236 1138 MslI CAynn'nnrTG Cuts at: 0 370 553 643 985 1072 1374 Size: 370 183 90 342 87 302 Fragments arranged by size: 370 342 302 183 90 87 MspA1I CmG'CkG Cuts at: 0 358 1101 1209 1374 Size: 358 743 108 165 Fragments arranged by size: 743 358 165 108 NarI GG'CG_CC Cuts at: 0 632 1131 1374 Size: 632 499 243 Fragments arranged by size: 632 499 243 NotI GC'GGCC_GC Cuts at: 0 402 1374 Size: 402 972 NspI r_CATG'y Cuts at: 0 477 1374 Size: 477 897 PciI A'CATG_T Cuts at: 0 473 1374 Size: 473 901 PflMI CCAn_nnn'nTGG Cuts at: 0 243 666 1354 1374 Size: 243 423 688 20 Fragments arranged by size: 688 423 243 20 PshAI GACnn'nnGTC Cuts at: 0 616 1374 Size: 616 758 PspOMI G'GGCC_C Cuts at: 0 41 1374 Size: 41 1333 PvuII CAG'CTG Cuts at: 0 1209 1374 Size: 1209 165 SacII CC_GC'GG Cuts at: 0 1102 1374 Size: 1102 272 SapI GCTCTTCn'nnn_ Cuts at: 0 313 907 1374 Size: 313 594 467 Fragments arranged by size: 594 467 313 SexAI A'CCwGG_T Cuts at: 0 881 1374 Size: 881 493 SfiI GGCCn_nnn'nGGCC Cuts at: 0 49 1374 Size: 49 1325 SfoI GGC'GCC Cuts at: 0 633 1132 1374 Size: 633 499 242 Fragments arranged by size: 633 499 242 TatI w'GTAC_w Cuts at: 0 446 593 1374 Size: 446 147 781 Fragments arranged by size: 781 446 147 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 808 840 1374 Size: 808 32 534 Fragments arranged by size: 808 534 32 Tth111I GACn'n_nGTC Cuts at: 0 723 883 1374 Size: 723 160 491 Fragments arranged by size: 723 491 160 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 465 978 1374 Size: 465 513 396 Fragments arranged by size: 513 465 396 Enzymes that do cut: AarI Acc65I AcuI AfeI AflIII AleI AlfI AlwNI ApaI ApaLI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BclI BglI BglII Bme1580I BmrI BplI BpmI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrDI BsrFI BsrGI BssSI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I FspI HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I NarI NotI NspI PciI PflMI PshAI PspOMI PvuII SacII SapI SexAI SfiI SfoI TatI TstI Tth111I XcmI Enzymes that do not cut: AatII AccI AclI AflII AgeI AhdI AloI ApoI AscI AseI AsiSI AvrII BbvCI BciVI BfrBI BlpI BmgBI BmtI Bpu10I BpuEI BsaAI BsaBI BsiWI BspHI BsrBI BssHII BstAPI BstBI BstEII BstXI BstZ17I ClaI CspCI DraI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI MfeI MluI NaeI NcoI NdeI NgoMIV NheI NruI NsiI PacI PasI PfoI PmeI PmlI PpiI PpuMI PsiI PspXI PsrI PstI PvuI RsrII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII XbaI XhoI XmaI XmnI ZraI