################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AgeI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BcnI BfrI BglI BlnI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmFI BspDI BspEI BspHI BsrBI BsrFI BssHII Bst1107I BstAPI BstBI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI EagI EarI EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MroI MspI MunI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI Tth111I TthHB8I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AflIII 527 ACATGT A^C[GA][CT]GT AhdI 1186 GACTCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 106 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG AspEI 1186 GACTCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 347 CCCGAG C^[CT]CG[GA]G AvaII 1065 GGTCC G^G[AT]CC BalI 837 TGGCCA TGG^CCA BanII 559 GAGCTC G[GA]GC[CT]^C BciVI 92 GTATCC GTATCC(6/5) BclI 543 TGATCA T^GATCA BfaI 1138 CTAG C^TAG BglII 446 AGATCT A^GATCT BlpI 934 GCTCAGC GC^T[ACGT]AGC Bpu1102I 934 GCTCAGC GC^T[ACGT]AGC BsaI 509 GGTCTC GGTCTC(1/5) BsgI 308 GTGCAG GTGCAG(16/14) BsmI 100 GAATGC GAATGC(1/-1) BsoBI 347 CCCGAG C^[CT]CG[GA]G Bsp1407I 941 TGTACA T^GTACA BspLU11I 527 ACATGT A^CATGT BspMI 902 ACCTGC ACCTGC(4/8) BsrDI 605 GCAATG GCAATG(2/0) BsrGI 941 TGTACA T^GTACA BssSI 1082 CACGAG CACGAG(-5/-1) BstEII 255 GGTCACC G^GT[ACGT]ACC BstPI 255 GGTCACC G^GT[ACGT]ACC Cac8I 687 GCTGGC GC[ACGT]^[ACGT]GC CelII 934 GCTCAGC GC^T[ACGT]AGC CfoI 567 GCGC GCG^C DraII 693 AGGGCCT [GA]G^G[ACGT]CC[CT] DsaI 702 CCATGG C^C[GA][CT]GG EaeI 837 TGGCCA [CT]^GGCC[GA] Eam1105I 1186 GACTCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 144 CCTGCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 693 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoO65I 255 GGTCACC G^GT[ACGT]ACC FbaI 543 TGATCA T^GATCA HhaI 567 GCGC GCG^C HinP1I 567 GCGC G^CGC MaeI 1138 CTAG C^TAG MluNI 837 TGGCCA TGG^CCA MscI 837 TGGCCA TGG^CCA MseI 923 TTAA T^TAA NcoI 702 CCATGG C^CATGG NdeI 353 CATATG CA^TATG NspI 527 ACATGT [GA]CATG^[CT] PstI 745 CTGCAG CTGCA^G SacI 559 GAGCTC GAGCT^C SspBI 941 TGTACA T^GTACA TfiI 987 GAATC G^A[AT]TC Tru9I 923 TTAA T^TAA XbaI 1137 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccII 566 CGCG CG^CG 866 CGCG AciI 865 CCGC CCGC(-3/-1) 476 GCGG AocI 148 CCTGAGG CC^T[ACGT]AGG 1124 CCTTAGG AspHI 559 GAGCTC G[AT]GC[AT]^C 1158 GAGCAC BbsI 400 GAAGAC GAAGAC(2/6) 855 GAAGAC BpmI 1027 CTCCAG CTGGAG(16/14) 1113 CTGGAG BpuAI 400 GAAGAC GAAGAC(2/6) 855 GAAGAC BsiHKAI 559 GAGCTC G[AT]GC[AT]^C 1158 GAGCAC BssKI 501 CCTGG ^CC[ACGT]GG 829 CCAGG BstF5I 72 CATCC GGATG(2/0) 582 CATCC BstNI 501 CCTGG CC^[AT]GG 829 CCAGG BstUI 566 CGCG CG^CG 866 CGCG BstYI 411 GGATCT [GA]^GATC[CT] 446 AGATCT Bsu36I 148 CCTGAGG CC^T[ACGT]AGG 1124 CCTTAGG Eco81I 148 CCTGAGG CC^T[ACGT]AGG 1124 CCTTAGG EcoRII 501 CCTGG ^CC[AT]GG 829 CCAGG FokI 72 CATCC GGATG(9/13) 582 CATCC MflI 411 GGATCT [GA]^GATC[CT] 446 AGATCT MslI 522 CACCAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 595 CATGAGGGTG MvaI 501 CCTGG CC^[AT]GG 829 CCAGG MvnI 566 CGCG CG^CG 866 CGCG NlaIV 484 GGGGCC GG[ACGT]^[ACGT]CC 1115 GGAGCC ScaI 380 AGTACT AGT^ACT 846 AGTACT ScrFI 501 CCTGG CC^[ACGT]GG 829 CCAGG SfaNI 1006 GATGC GCATC(5/9) 71 GCATC SfcI 462 CTACAG C^T[GA][CT]AG 745 CTGCAG Tsp509I 517 AATT ^AATT 1054 AATT XhoII 411 GGATCT [GA]^GATC[CT] 446 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AfaI AluI AlwI BbvI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BstXI Cfr13I DdeI DpnII EcoT14I Fnu4HI HaeIII HinfI HphI ItaI MaeIII MboI MboII MnlI MspA1I MwoI NdeII NlaIII PflMI PleI PvuII RsaI Sau3AI Sau96I StyI TseI Tsp45I TspRI Van91I XcmI