Restriction map for
pFN21AE5548
(Circular) (Six-base) MAPSORT of: pFN21AE5548.seq Check: 2155 from: 1 to: 5905
pFN21AE5548 5905 bp
With 182 enzymes: SgfI *
January 30, 2014 17:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2916 2916
Size: 5905
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3715 278
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3248 1937
Size: 1311 4594
Acc65I G'GTAC_C
Cuts at: 3226 3226
Size: 5905
AclI AA'CG_TT
Cuts at: 1563 4032 4405 1563
Size: 2469 373 3063
Fragments arranged by size:
3063 2469 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3968 5352 5620 1632
Size: 2336 1384 268 1917
Fragments arranged by size:
2336 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5708
AflIII A'CryG_T
Cuts at: 1203 2532 4804 5781 1203
Size: 1329 2272 977 1327
Fragments arranged by size:
2272 1329 1327 977
AgeI A'CCGG_T
Cuts at: 4736 4736
Size: 5905
AhdI GACnn_n'nnGTC
Cuts at: 3196 4634 3196
Size: 1438 4467
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5905
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5905
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2814 2846 2814
Size: 32 5873
AlwNI CAG_nnn'CTG
Cuts at: 2116 3648 5220 2116
Size: 1532 1572 2801
Fragments arranged by size:
2801 1572 1532
ApaLI G'TGCA_C
Cuts at: 3962 5118 3962
Size: 1156 4749
ApoI r'AATT_y
Cuts at: 1493 3214 3440 3592 5701 1493
Size: 1721 226 152 2109 1697
Fragments arranged by size:
2109 1721 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4459 160
Size: 872 3427 1606
Fragments arranged by size:
3427 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
AvaI C'yCGr_G
Cuts at: 1870 1943 2352 3230 1870
Size: 73 409 878 4545
Fragments arranged by size:
4545 878 409 73
BamHI G'GATC_C
Cuts at: 3235 3602 3235
Size: 367 5538
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3226 4681 618
Size: 325 217 627 1439 1455 1842
Fragments arranged by size:
1842 1455 1439 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2568 3224 1391
Size: 1177 656 4072
Fragments arranged by size:
4072 1177 656
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2412 2867 928
Size: 971 513 455 3966
Fragments arranged by size:
3966 971 513 455
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4096 4130 1903
Size: 34 2159 34 3678
Fragments arranged by size:
3678 2159 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2108 3797 5007 2108
Size: 1689 1210 3006
Fragments arranged by size:
3006 1689 1210
BclI T'GATC_A
Cuts at: 1548 2548 1548
Size: 1000 4905
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4516 136
Size: 107 122 71 631 605 2844 1525
Fragments arranged by size:
2844 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2451 5900 2451
Size: 3449 2456
BlpI GC'TnA_GC
Cuts at: 2940 3322 2940
Size: 382 5523
Bme1580I G_kGCm'C
Cuts at: 1790 2623 2650 2961 3966 5122 1790
Size: 833 27 311 1005 1156 2573
Fragments arranged by size:
2573 1156 1005 833 311 27
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5905
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2398 3636 4594
Size: 416 53 83 399 24 981 1238 958
Cuts at: 4594 442
Size: 1753
Fragments arranged by size:
1753 1238 981 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5905
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2259 2259
Size: 5905
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3017 3139 4565 1239
Size: 1778 122 1426 2579
Fragments arranged by size:
2579 1778 1426 122
Bpu10I CC'TnA_GC
Cuts at: 2130 2130
Size: 5905
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4030 4895 5193 4030
Size: 865 298 4742
Fragments arranged by size:
4742 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2520 4568 882
Size: 617 1021 2048 2219
Fragments arranged by size:
2219 2048 1021 617
BsaAI yAC'GTr
Cuts at: 493 1204 3696 493
Size: 711 2492 2702
Fragments arranged by size:
2702 2492 711
BsaBI GATnn'nnATC
Cuts at: 5899 5899
Size: 5905
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3712 4094 4729 275
Size: 53 83 186 936 2179 382 635 1451
Fragments arranged by size:
2179 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4338 4736 5010 5157 5568 4338
Size: 398 274 147 411 4675
Fragments arranged by size:
4675 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5680
Fragments arranged by size:
5680 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5905
BseYI C'CCAG_C
Cuts at: 5108 5604 5108
Size: 496 5409
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2334 1163
Size: 573 598 4734
Fragments arranged by size:
4734 598 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3355 4116 5144 1540
Size: 275 186 1354 761 1028 2301
Fragments arranged by size:
2301 1354 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2568 3167 3966 4051 5122 2568
Size: 599 799 85 1071 3351
Fragments arranged by size:
3351 1071 799 599 85
BsmI GAATG_Cn'
Cuts at: 2111 3416 3509 2111
Size: 1305 93 4507
Fragments arranged by size:
4507 1305 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2568 2623 2650 2961 3167 3224 3966
Size: 399 778 55 27 311 206 57 742
Cuts at: 3966 4051 5122 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 778 742 399 311 206 85
57 55 27
BspEI T'CCGG_A
Cuts at: 5568 5568
Size: 5905
BspHI T'CATG_A
Cuts at: 3794 5524 3794
Size: 1730 4175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2916 3260 844
Size: 2072 344 3489
Fragments arranged by size:
3489 2072 344
BsrBI CCG'CTC
Cuts at: 1394 1823 3792 1394
Size: 429 1969 3507
Fragments arranged by size:
3507 1969 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2617 4400 4574 65
Size: 1183 1369 1783 174 1396
Fragments arranged by size:
1783 1396 1369 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4549 4736 1700
Size: 110 2739 187 2869
Fragments arranged by size:
2869 2739 187 110
BsrGI T'GTAC_A
Cuts at: 96 2946 96
Size: 2850 3055
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5905
BssSI C'ACGA_G
Cuts at: 3087 3965 4977 3087
Size: 878 1012 4015
Fragments arranged by size:
4015 1012 878
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5905
BstBI TT'CG_AA
Cuts at: 4709 4709
Size: 5905
BstEII G'GTnAC_C
Cuts at: 1184 2260 1184
Size: 1076 4829
BstXI CCAn_nnnn'nTGG
Cuts at: 2606 2635 3041 2606
Size: 29 406 5470
Fragments arranged by size:
5470 406 29
BstYI r'GATC_y
Cuts at: 1972 2416 2451 3235 3602 3987 4004 5456 5542
Size: 444 35 784 367 385 17 1452 86
Cuts at: 5542 5554 5900 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 784 444 385 367 346 86
35 17 12
Bsu36I CC'TnA_GG
Cuts at: 2154 3130 2154
Size: 976 4929
BtgI C'CryG_G
Cuts at: 513 1065 2707 513
Size: 552 1642 3711
Fragments arranged by size:
3711 1642 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5166
Fragments arranged by size:
5166 683 56
BtsI GCAGTG_nn'
Cuts at: 2650 3421 4215 4235 2650
Size: 771 794 20 4320
Fragments arranged by size:
4320 794 771 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5905
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5870
DraI TTT'AAA
Cuts at: 3209 3209
Size: 5905
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4912 809
Size: 507 3596 1802
Fragments arranged by size:
3596 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2842 3352 4241
Size: 54 1425 50 275 18 1012 510 889
Cuts at: 4241 5870 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1012 889 510 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3352 1537
Size: 275 1540 4090
Fragments arranged by size:
4090 1540 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3835 1353
Size: 2482 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4492 4866 5012 387
Size: 1064 138 2903 374 146 1280
Fragments arranged by size:
2903 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2566 2566
Size: 5905
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3017 3139 3968 4565 5352 5620 1239
Size: 393 1385 122 829 597 787 268 1524
Fragments arranged by size:
1524 1385 829 787 597 393 268 122
EcoNI CCTnn'n_nnAGG
Cuts at: 2153 2153
Size: 5905
EcoO109I rG'GnC_Cy
Cuts at: 2699 2699
Size: 5905
EcoRI G'AATT_C
Cuts at: 3214 3592 3214
Size: 378 5527
FspI TGC'GCA
Cuts at: 4411 4411
Size: 5905
HaeII r_GCGC'y
Cuts at: 1678 5052 1678
Size: 3374 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2784 2816 3182 3214 4546
Size: 32 135 32 1212 32 366 32 1332
Cuts at: 4546 4578 4620 4652 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1332 1212 366 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3249 3495 669
Size: 988 281 1311 246 3079
Fragments arranged by size:
3079 1311 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5905
HpaI GTT'AAC
Cuts at: 3495 3495
Size: 5905
KpnI G_GTAC'C
Cuts at: 3230 3230
Size: 5905
MfeI C'AATT_G
Cuts at: 3504 3504
Size: 5905
MluI A'CGCG_T
Cuts at: 5781 5781
Size: 5905
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2679 5019 5203 1305
Size: 1374 2340 184 2007
Fragments arranged by size:
2340 2007 1374 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2844 10
Size: 54 1425 343 1012 3071
Fragments arranged by size:
3071 1425 1012 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2531 2604 3863 4222 4381 518
Size: 648 1365 73 1259 359 159 2042
Fragments arranged by size:
2042 1365 1259 648 359 159 73
MspA1I CmG'CkG
Cuts at: 1606 2318 2481 2749 2944 3321 3998 5146 5391
Size: 712 163 268 195 377 677 1148 245
Cuts at: 5391 1606
Size: 2120
Fragments arranged by size:
2120 1148 712 677 377 268 245 195
163
NcoI C'CATG_G
Cuts at: 513 1065 2707 513
Size: 552 1642 3711
Fragments arranged by size:
3711 1642 552
NdeI CA'TA_TG
Cuts at: 387 2359 387
Size: 1972 3933
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5905
NotI GC'GGCC_GC
Cuts at: 3352 3352
Size: 5905
NspI r_CATG'y
Cuts at: 1132 1229 2536 3263 4808 1132
Size: 97 1307 727 1545 2229
Fragments arranged by size:
2229 1545 1307 727 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5905
PciI A'CATG_T
Cuts at: 2532 4804 2532
Size: 2272 3633
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2388 2638 2713 2840 1108
Size: 1280 250 75 127 4173
Fragments arranged by size:
4173 1280 250 127 75
PmeI GTTT'AAAC
Cuts at: 3209 3209
Size: 5905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3974 4006 5487 5519 3974
Size: 32 1481 32 4360
Fragments arranged by size:
4360 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5905
PsiI TTA'TAA
Cuts at: 3475 3475
Size: 5905
PstI C_TGCA'G
Cuts at: 830 2754 3257 830
Size: 1924 503 3478
Fragments arranged by size:
3478 1924 503
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5905
PvuII CAG'CTG
Cuts at: 2318 2749 2944 2318
Size: 431 195 5279
Fragments arranged by size:
5279 431 195
SacI G_AGCT'C
Cuts at: 2568 2568
Size: 5905
SalI G'TCGA_C
Cuts at: 1936 3247 1936
Size: 1311 4594
SbfI CC_TGCA'GG
Cuts at: 3257 3257
Size: 5905
ScaI AGT'ACT
Cuts at: 2387 2853 4153 5865 2387
Size: 466 1300 1712 2427
Fragments arranged by size:
2427 1712 1300 466
SfcI C'TryA_G
Cuts at: 826 1045 2467 2750 3253 4388 5069 5260 826
Size: 219 1422 283 503 1135 681 191 1471
Fragments arranged by size:
1471 1422 1135 681 503 283 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5905
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5905
SmaI CCC'GGG
Cuts at: 1872 3232 1872
Size: 1360 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4009 4910 5172 820
Size: 197 926 2066 901 262 1553
Fragments arranged by size:
2066 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3696 493
Size: 3203 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5905
SphI G_CATG'C
Cuts at: 1132 3263 1132
Size: 2131 3774
SspI AAT'ATT
Cuts at: 5 52 3829 5
Size: 47 3777 2081
Fragments arranged by size:
3777 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5905
StyI C'CwwG_G
Cuts at: 513 1065 2124 2707 2996 3344 513
Size: 552 1059 583 289 348 3074
Fragments arranged by size:
3074 1059 583 552 348 289
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3086 3932 3949 4102 4287 4732 325
Size: 1255 1506 846 17 153 185 445 1498
Fragments arranged by size:
1506 1498 1255 846 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2385 2851 2946
Size: 274 80 33 51 1443 408 466 95
Cuts at: 2946 3100 4151 5863 96
Size: 154 1051 1712 138
Fragments arranged by size:
1712 1443 1051 466 408 274 154 138
95 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5865 5897 5865
Size: 32 5873
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5905
XbaI T'CTAG_A
Cuts at: 3142 3241 3142
Size: 99 5806
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2635 2689 2929 2635
Size: 54 240 5611
Fragments arranged by size:
5611 240 54
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5905
XmaI C'CCGG_G
Cuts at: 1870 3230 1870
Size: 1360 4545
XmnI GAAnn'nnTTC
Cuts at: 4034 4034
Size: 5905
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3713 276
Size: 53 83 186 936 2179 2468
Fragments arranged by size:
2468 2179 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI