################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BcnI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HapII HhaI Hin1I HinP1I HincII HindII HpaI HpaII KasI KpnI KspI MaeII MamI MluI MluNI MroI MscI MspA1I MspI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI SspBI StuI SwaI Tth111I Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AcsI 520 GAATTT [GA]^AATT[CT] AflII 580 CTTAAG C^TTAAG AlwNI 397 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 430 CCTCAGG CC^T[ACGT]AGG ApoI 520 GAATTT [GA]^AATT[CT] BanI 435 GGTGCC G^G[CT][GA]CC BanII 230 GGGCTC G[GA]GC[CT]^C BbvI 396 GCAGC GCAGC(8/12) BclI 168 TGATCA T^GATCA BfrI 580 CTTAAG C^TTAAG BglII 750 AGATCT A^GATCT BsaI 253 GAGACC GGTCTC(1/5) BsaJI 140 CCATGG C^C[ACGT][ACGT]GG BssKI 207 CCAGG ^CC[ACGT]GG BstEII 333 GGTGACC G^GT[ACGT]ACC BstNI 207 CCAGG CC^[AT]GG BstPI 333 GGTGACC G^GT[ACGT]ACC BstXI 257 CCACGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 430 CCTCAGG CC^T[ACGT]AGG DsaI 140 CCATGG C^C[GA][CT]GG EarI 460 GAAGAG CTCTTC(1/4) Eco81I 430 CCTCAGG CC^T[ACGT]AGG EcoO65I 333 GGTGACC G^GT[ACGT]ACC EcoRII 207 CCAGG ^CC[AT]GG EcoT14I 140 CCATGG C^C[AT][AT]GG FbaI 168 TGATCA T^GATCA Fnu4HI 396 GCAGC GC^[ACGT]GC HaeIII 159 GGCC GG^CC HgaI 377 GACGC GACGC(5/10) HindIII 832 AAGCTT A^AGCTT ItaI 396 GCAGC GC^[ACGT]GC Ksp632I 460 GAAGAG CTCTTC(1/4) MaeIII 334 GTGAC ^GT[ACGT]AC MfeI 593 CAATTG C^AATTG MslI 306 CACTGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 593 CAATTG C^AATTG MvaI 207 CCAGG CC^[AT]GG NcoI 140 CCATGG C^CATGG NlaIV 435 GGTGCC GG[ACGT]^[ACGT]CC SbfI 327 CCTGCAGG CCTGCA^GG ScrFI 207 CCAGG CC^[ACGT]GG SmlI 580 CTTAAG C^T[CT][GA]AG SpeI 652 ACTAGT A^CTAGT Sse8387I 327 CCTGCAGG CCTGCA^GG SspI 814 AATATT AAT^ATT StyI 140 CCATGG C^C[AT][AT]GG TseI 396 GCAGC G^C[AT]GC Tsp45I 334 GTGAC ^GT[GC]AC XbaI 480 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AfaI 225 GTAC GT^AC 504 GTAC AluI 99 AGCT AG^CT 833 AGCT AlwI 93 GATCC GGATC(4/5) 423 GGATC BbsI 544 GAAGAC GAAGAC(2/6) 445 GTCTTC BmyI 230 GGGCTC G[AGT]GC[ACT]^C 838 GTGCCC BpuAI 544 GAAGAC GAAGAC(2/6) 445 GTCTTC BsmAI 253 GAGAC GTCTC(1/5) 203 GTCTC Bsp1286I 230 GGGCTC G[AGT]GC[ACT]^C 838 GTGCCC BspMI 623 ACCTGC ACCTGC(4/8) 330 GCAGGT BsrI 227 ACTGG ACTGG(1/-1) 307 ACTGG BstYI 92 AGATCC [GA]^GATC[CT] 750 AGATCT MflI 92 AGATCC [GA]^GATC[CT] 750 AGATCT MwoI 429 GCCTCAGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 825 GCAGAGAAAGC PleI 768 GACTC GAGTC(4/5) 607 GAGTC PstI 328 CTGCAG CTGCA^G 625 CTGCAG RsaI 225 GTAC GT^AC 504 GTAC SfaNI 136 GATGC GCATC(5/9) 364 GATGC SfcI 328 CTGCAG C^T[GA][CT]AG 625 CTGCAG TaqI 362 TCGA T^CGA 588 TCGA TspRI 306 CACTG CA[GC]TG[ACGT][ACGT]^ 694 CACTG TthHB8I 362 TCGA T^CGA 588 TCGA XhoII 92 AGATCC [GA]^GATC[CT] 750 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### Asp700I BfaI BpmI BsiYI BslI BstF5I DdeI DpnII FokI HinfI HphI MaeI MboI MboII MnlI MseI NdeII NlaIII Sau3AI TfiI Tru9I Tsp509I XmnI