Restriction map for
pFN21AE5349
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaII
AvrII BalI BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmFI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
Cfr13I ClaI CpoI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HaeIII HapII HgaI
Hin1I HincII HindII HpaI HpaII
KasI KpnI Ksp632I KspI MamI
MluI MluNI MroI MscI MspI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI Sau96I
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I Van91I XcmI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 838 GAATTT [GA]^AATT[CT]
ApoI 838 GAATTT [GA]^AATT[CT]
AspHI 539 GAGCAC G[AT]GC[AT]^C
AvaI 648 CTCGAG C^[CT]CG[GA]G
AviII 575 TGCGCA TGC^GCA
BanI 463 GGTGCC G^G[CT][GA]CC
BanII 27 GGGCTC G[GA]GC[CT]^C
BbsI 403 GAAGAC GAAGAC(2/6)
BpmI 625 CTGGAG CTGGAG(16/14)
BpuAI 403 GAAGAC GAAGAC(2/6)
BseRI 4 GAGGAG GAGGAG(10/8)
BsgI 777 CTGCAC GTGCAG(16/14)
BsiHKAI 539 GAGCAC G[AT]GC[AT]^C
BsiYI 151 CCTCCTGTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 151 CCTCCTGTAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 588 GTCTC GTCTC(1/5)
BsoBI 648 CTCGAG C^[CT]CG[GA]G
BsrI 104 ACTGG ACTGG(1/-1)
BssKI 742 CCTGG ^CC[ACGT]GG
BstNI 742 CCTGG CC^[AT]GG
DraI 448 TTTAAA TTT^AAA
EcoNI 151 CCTCCTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 742 CCTGG ^CC[AT]GG
EcoT14I 865 CCAAGG C^C[AT][AT]GG
FauI 24 GCGGG CCCGC(4/6)
FspI 575 TGCGCA TGC^GCA
HaeII 37 GGCGCT [GA]GCGC^[CT]
HindIII 828 AAGCTT A^AGCTT
MaeII 330 ACGT A^CGT
MfeI 323 CAATTG C^AATTG
MslI 787 CATATTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 622 CAGCTG C[AC]G^C[GT]G
MunI 323 CAATTG C^AATTG
MvaI 742 CCTGG CC^[AT]GG
NdeI 544 CATATG CA^TATG
PaeR7I 648 CTCGAG C^TCGAG
PleI 570 GAGTC GAGTC(4/5)
PvuII 622 CAGCTG CAG^CTG
ScrFI 742 CCTGG CC^[ACGT]GG
SfcI 155 CTGTAG C^T[GA][CT]AG
SmlI 648 CTCGAG C^T[CT][GA]AG
SspI 718 AATATT AAT^ATT
StyI 865 CCAAGG C^C[AT][AT]GG
XbaI 762 TCTAGA T^CTAGA
XhoI 648 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AciI 24 GCGG CCGC(-3/-1)
433 GCGG
AfaI 704 GTAC GT^AC
739 GTAC
AlwI 484 GATCC GGATC(4/5)
801 GGATC
BglI 111 GCCTGATAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
424 GCCAAAGAGGC
BglII 337 AGATCT A^GATCT
681 AGATCT
BmyI 27 GGGCTC G[AGT]GC[ACT]^C
539 GAGCAC
BsaJI 742 CCTGGG C^C[ACGT][ACGT]GG
865 CCAAGG
Bsp1286I 27 GGGCTC G[AGT]GC[ACT]^C
539 GAGCAC
BspMI 357 ACCTGC ACCTGC(4/8)
775 ACCTGC
BstAPI 292 GCATGTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
457 GCAGTTGGTGC
BstYI 337 AGATCT [GA]^GATC[CT]
681 AGATCT
CfoI 38 GCGC GCG^C
576 GCGC
HhaI 38 GCGC GCG^C
576 GCGC
HinP1I 38 GCGC G^CGC
576 GCGC
HphI 265 GGTGA GGTGA(8/7)
527 TCACC
MflI 337 AGATCT [GA]^GATC[CT]
681 AGATCT
MseI 344 TTAA T^TAA
449 TTAA
NlaIV 28 GGCTCC GG[ACGT]^[ACGT]CC
463 GGTGCC
NspI 292 GCATGT [GA]CATG^[CT]
557 ACATGC
RsaI 704 GTAC GT^AC
739 GTAC
SfaNI 298 GATGC GCATC(5/9)
808 GATGC
TaqI 649 TCGA T^CGA
836 TCGA
Tru9I 344 TTAA T^TAA
449 TTAA
TspRI 750 CACTG CA[GC]TG[ACGT][ACGT]^
812 CAGTG
TthHB8I 649 TCGA T^CGA
836 TCGA
XhoII 337 AGATCT [GA]^GATC[CT]
681 AGATCT
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AluI BbvI BfaI BspHI BstF5I
Cac8I DdeI DpnII Fnu4HI FokI
HinfI ItaI MaeI MaeIII MboI
MboII MnlI MwoI NdeII NlaIII
RcaI Sau3AI TfiI TseI Tsp45I
Tsp509I