(Circular) (Six-base) MAPSORT of: pFN21AE5349.seq Check: 3537 from: 1 to: 5596 pFN21AE5349 5596 bp With 182 enzymes: SgfI * January 30, 2014 17:18 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3406 278 Size: 53 83 186 936 1870 2468 Fragments arranged by size: 2468 1870 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2939 1937 Size: 1002 4594 Acc65I G'GTAC_C Cuts at: 2917 2917 Size: 5596 AclI AA'CG_TT Cuts at: 1563 3723 4096 1563 Size: 2160 373 3063 Fragments arranged by size: 3063 2160 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2871 3659 5043 5311 1632 Size: 1239 788 1384 268 1917 Fragments arranged by size: 1917 1384 1239 788 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5399 AflIII A'CryG_T Cuts at: 1203 4495 5472 1203 Size: 3292 977 1327 Fragments arranged by size: 3292 1327 977 AgeI A'CCGG_T Cuts at: 4427 4427 Size: 5596 AhdI GACnn_n'nnGTC Cuts at: 4325 4325 Size: 5596 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5596 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2442 1184 Size: 1258 4338 AlwNI CAG_nnn'CTG Cuts at: 3339 4911 3339 Size: 1572 4024 ApaLI G'TGCA_C Cuts at: 3653 4809 3653 Size: 1156 4440 ApoI r'AATT_y Cuts at: 1493 2843 2905 3131 3283 5392 1493 Size: 1350 62 226 152 2109 1697 Fragments arranged by size: 2109 1697 1350 226 152 62 AseI AT'TA_AT Cuts at: 160 1032 4150 160 Size: 872 3118 1606 Fragments arranged by size: 3118 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5596 AvaI C'yCGr_G Cuts at: 1870 1943 2653 2921 1870 Size: 73 710 268 4545 Fragments arranged by size: 4545 710 268 73 BamHI G'GATC_C Cuts at: 2926 3293 2926 Size: 367 5229 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2468 2917 4372 618 Size: 325 217 627 681 449 1455 1842 Fragments arranged by size: 1842 1455 681 627 449 325 217 BanII G_rGCy'C Cuts at: 1391 2036 2915 1391 Size: 645 879 4072 Fragments arranged by size: 4072 879 645 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2415 928 Size: 971 516 4109 Fragments arranged by size: 4109 971 516 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3787 3821 1903 Size: 34 1850 34 3678 Fragments arranged by size: 3678 1850 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3488 4698 3488 Size: 1210 4386 BclI T'GATC_A Cuts at: 1548 1548 Size: 5596 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2122 2435 4207 Size: 107 122 71 631 605 450 313 1772 Cuts at: 4207 136 Size: 1525 Fragments arranged by size: 1772 1525 631 605 450 313 122 107 71 BglII A'GATC_T Cuts at: 2342 2686 5591 2342 Size: 344 2905 2347 Fragments arranged by size: 2905 2347 344 BlpI GC'TnA_GC Cuts at: 3013 3013 Size: 5596 Bme1580I G_kGCm'C Cuts at: 1790 3657 4813 1790 Size: 1867 1156 2573 Fragments arranged by size: 2573 1867 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5596 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3327 4285 442 Size: 416 53 83 399 24 1910 958 1753 Fragments arranged by size: 1910 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5596 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2651 4256 1239 Size: 1412 1605 2579 Fragments arranged by size: 2579 1605 1412 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3721 4586 4884 3721 Size: 865 298 4433 Fragments arranged by size: 4433 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4259 882 Size: 617 2760 2219 Fragments arranged by size: 2760 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3387 493 Size: 711 2183 2702 Fragments arranged by size: 2702 2183 711 BsaBI GATnn'nnATC Cuts at: 5590 5590 Size: 5596 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3403 3785 4420 275 Size: 53 83 186 936 1870 382 635 1451 Fragments arranged by size: 1870 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4029 4427 4701 4848 5259 4029 Size: 398 274 147 411 4366 Fragments arranged by size: 4366 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5371 Fragments arranged by size: 5371 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 1186 Size: 838 4758 BseYI C'CCAG_C Cuts at: 4799 5295 4799 Size: 496 5100 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2767 1163 Size: 573 1031 3992 Fragments arranged by size: 3992 1031 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3046 3807 4835 1540 Size: 275 186 1045 761 1028 2301 Fragments arranged by size: 2301 1045 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2548 3657 3742 4813 2548 Size: 1109 85 1071 3331 Fragments arranged by size: 3331 1109 1071 85 BsmI GAATG_Cn' Cuts at: 3107 3200 3107 Size: 93 5503 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 2548 2915 3657 3742 4813 1391 Size: 399 246 512 367 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 512 399 367 246 85 BspEI T'CCGG_A Cuts at: 5259 5259 Size: 5596 BspHI T'CATG_A Cuts at: 2074 2137 2662 3485 5215 2074 Size: 63 525 823 1730 2455 Fragments arranged by size: 2455 1730 823 525 63 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2371 2789 2951 844 Size: 1527 418 162 3489 Fragments arranged by size: 3489 1527 418 162 BsrBI CCG'CTC Cuts at: 1394 1823 3483 1394 Size: 429 1660 3507 Fragments arranged by size: 3507 1660 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4091 4265 65 Size: 1183 2843 174 1396 Fragments arranged by size: 2843 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4240 4427 1700 Size: 110 2430 187 2869 Fragments arranged by size: 2869 2430 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5596 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5596 BssSI C'ACGA_G Cuts at: 3656 4668 3656 Size: 1012 4584 BstAPI GCAn_nnn'nTGC Cuts at: 794 2303 2468 794 Size: 1509 165 3922 Fragments arranged by size: 3922 1509 165 BstBI TT'CG_AA Cuts at: 4400 4400 Size: 5596 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5596 BstYI r'GATC_y Cuts at: 1972 2342 2686 2926 3293 3678 3695 5147 5233 Size: 370 344 240 367 385 17 1452 86 Cuts at: 5233 5245 5591 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 370 367 346 344 240 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4105 Fragments arranged by size: 4105 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4857 Fragments arranged by size: 4857 683 56 BtsI GCAGTG_nn' Cuts at: 2754 2823 3112 3906 3926 2754 Size: 69 289 794 20 4424 Fragments arranged by size: 4424 794 289 69 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5596 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5561 DraI TTT'AAA Cuts at: 2455 2900 2455 Size: 445 5151 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4603 809 Size: 507 3287 1802 Fragments arranged by size: 3287 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3043 3932 5561 Size: 54 1425 50 275 18 1213 889 1629 Cuts at: 5561 8 Size: 43 Fragments arranged by size: 1629 1425 1213 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3043 1537 Size: 275 1231 4090 Fragments arranged by size: 4090 1231 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3526 1353 Size: 2173 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4183 4557 4703 387 Size: 1064 138 2594 374 146 1280 Fragments arranged by size: 2594 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2651 2871 3659 4256 5043 5311 1239 Size: 393 1019 220 788 597 787 268 1524 Fragments arranged by size: 1524 1019 788 787 597 393 268 220 EcoNI CCTnn'n_nnAGG Cuts at: 2160 2160 Size: 5596 EcoRI G'AATT_C Cuts at: 2905 3283 2905 Size: 378 5218 FspI TGC'GCA Cuts at: 2582 4102 2582 Size: 1520 4076 HaeII r_GCGC'y Cuts at: 1678 2046 4743 1678 Size: 368 2697 2531 Fragments arranged by size: 2697 2531 368 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4237 4269 4311 4343 1373 Size: 32 135 32 2665 32 42 32 2626 Fragments arranged by size: 2665 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2940 3186 669 Size: 988 281 1002 246 3079 Fragments arranged by size: 3079 1002 988 281 246 HindIII A'AGCT_T Cuts at: 748 2833 748 Size: 2085 3511 HpaI GTT'AAC Cuts at: 3186 3186 Size: 5596 KpnI G_GTAC'C Cuts at: 2921 2921 Size: 5596 MfeI C'AATT_G Cuts at: 2328 3195 2328 Size: 867 4729 MluI A'CGCG_T Cuts at: 5472 5472 Size: 5596 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4710 4894 1305 Size: 3405 184 2007 Fragments arranged by size: 3405 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3774 Fragments arranged by size: 3774 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2796 3554 3913 4072 518 Size: 648 1630 758 359 159 2042 Fragments arranged by size: 2042 1630 758 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2629 3012 3689 4837 5082 1606 Size: 1023 383 677 1148 245 2120 Fragments arranged by size: 2120 1148 1023 677 383 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4105 Fragments arranged by size: 4105 939 552 NdeI CA'TA_TG Cuts at: 387 2550 387 Size: 2163 3433 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5596 NotI GC'GGCC_GC Cuts at: 3043 3043 Size: 5596 NspI r_CATG'y Cuts at: 1132 1229 2301 2566 2954 4499 1132 Size: 97 1072 265 388 1545 2229 Fragments arranged by size: 2229 1545 1072 388 265 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5596 PciI A'CATG_T Cuts at: 4495 4495 Size: 5596 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5596 PmeI GTTT'AAAC Cuts at: 2900 2900 Size: 5596 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3665 3697 5178 5210 3665 Size: 32 1481 32 4051 Fragments arranged by size: 4051 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5596 PsiI TTA'TAA Cuts at: 2169 3166 2169 Size: 997 4599 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2055 2087 2850 2882 2055 Size: 32 763 32 4769 Fragments arranged by size: 4769 763 32 32 PstI C_TGCA'G Cuts at: 830 2948 830 Size: 2118 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5596 PvuII CAG'CTG Cuts at: 2629 2629 Size: 5596 SalI G'TCGA_C Cuts at: 1936 2938 1936 Size: 1002 4594 SbfI CC_TGCA'GG Cuts at: 2948 2948 Size: 5596 ScaI AGT'ACT Cuts at: 3844 5556 3844 Size: 1712 3884 SfcI C'TryA_G Cuts at: 826 1045 2160 2944 4079 4760 4951 826 Size: 219 1115 784 1135 681 191 1471 Fragments arranged by size: 1471 1135 1115 784 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5596 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5596 SmaI CCC'GGG Cuts at: 1872 2923 1872 Size: 1051 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2653 3700 4601 4863 820 Size: 197 926 710 1047 901 262 1553 Fragments arranged by size: 1553 1047 926 901 710 262 197 SnaBI TAC'GTA Cuts at: 493 3387 493 Size: 2894 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5596 SphI G_CATG'C Cuts at: 1132 2954 1132 Size: 1822 3774 SspI AAT'ATT Cuts at: 5 52 2725 3520 5 Size: 47 2673 795 2081 Fragments arranged by size: 2673 2081 795 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5596 StyI C'CwwG_G Cuts at: 513 1065 2004 2870 3035 513 Size: 552 939 866 165 3074 Fragments arranged by size: 3074 939 866 552 165 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3623 3640 3793 3978 4423 325 Size: 1255 2043 17 153 185 445 1498 Fragments arranged by size: 2043 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3842 5554 96 Size: 274 80 33 51 1443 1865 1712 138 Fragments arranged by size: 1865 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5556 5588 5556 Size: 32 5564 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5596 XbaI T'CTAG_A Cuts at: 2767 2932 2767 Size: 165 5431 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5596 XhoI C'TCGA_G Cuts at: 1943 2653 1943 Size: 710 4886 XmaI C'CCGG_G Cuts at: 1870 2921 1870 Size: 1051 4545 XmnI GAAnn'nnTTC Cuts at: 3725 3725 Size: 5596 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3404 276 Size: 53 83 186 936 1870 2468 Fragments arranged by size: 2468 1870 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI