################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## AatII Acc65I AccI AclI AcyI AfaI AflII AflIII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII BalI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlpI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsgI BsiEI BsiWI BsmAI BsmBI BsoBI Bsp1407I BspDI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoRI EcoRV FseI FspI HaeII Hin1I KasI KpnI Ksp632I KspI MaeII MfeI MluI MluNI MscI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccII 108 CGCG CG^CG AccIII 744 TCCGGA T^CCGGA AciI 832 GCGG CCGC(-3/-1) Alw44I 431 GTGCAC G^TGCAC ApaLI 431 GTGCAC G^TGCAC AvrII 732 CCTAGG C^CTAGG BamHI 75 GGATCC G^GATCC BanII 783 GAGCCC G[GA]GC[CT]^C BclI 170 TGATCA T^GATCA BlnI 732 CCTAGG C^CTAGG BsaBI 1031 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 744 TCCGGA [AT]^CCGG[AT] BseAI 744 TCCGGA T^CCGGA BsmFI 188 GGGAC GGGAC(10/14) BspEI 744 TCCGGA T^CCGGA BspHI 417 TCATGA T^CATGA BsrFI 377 ACCGGC [GA]^CCGG[CT] BstAPI 193 GCATGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 339 GGTGACC G^GT[ACGT]ACC BstPI 339 GGTGACC G^GT[ACGT]ACC BstUI 108 CGCG CG^CG BstYI 75 GGATCC [GA]^GATC[CT] CfoI 109 GCGC GCG^C Cfr10I 377 ACCGGC [GA]^CCGG[CT] DraII 132 AGGACCT [GA]G^G[ACGT]CC[CT] EcoNI 213 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 132 AGGACCT [GA]G^G[ACGT]CC[CT] EcoO65I 339 GGTGACC G^GT[ACGT]ACC EcoT22I 692 ATGCAT ATGCA^T FauI 832 GCGGG CCCGC(4/6) FbaI 170 TGATCA T^GATCA HgaI 190 GACGC GACGC(5/10) HhaI 109 GCGC GCG^C HinP1I 109 GCGC G^CGC HincII 825 GTTAAC GT[CT]^[GA]AC HindII 825 GTTAAC GT[CT]^[GA]AC HindIII 925 AAGCTT A^AGCTT HpaI 825 GTTAAC GTT^AAC MamI 1031 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 75 GGATCC [GA]^GATC[CT] MroI 744 TCCGGA T^CCGGA MslI 405 CACTGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 108 CGCG CG^CG NsiI 692 ATGCAT ATGCA^T PpuMI 132 AGGACCT [GA]G^G[AT]CC[CT] PvuII 644 CAGCTG CAG^CTG RcaI 417 TCATGA T^CATGA SexAI 153 ACCAGGT A^CC[AT]GGT TaqI 680 TCGA T^CGA TthHB8I 680 TCGA T^CGA XhoII 75 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AlwI 76 GATCC GGATC(4/5) 75 GGATC AspHI 431 GTGCAC G[AT]GC[AT]^C 845 GTGCTC BfaI 284 CTAG C^TAG 733 CTAG BseRI 756 CTCCTC GAGGAG(10/8) 550 GAGGAG BsiHKAI 431 GTGCAC G[AT]GC[AT]^C 845 GTGCTC BsrDI 13 GCAATG GCAATG(2/0) 1013 GCAATG BstF5I 453 GGATG GGATG(2/0) 751 GGATG FokI 453 GGATG GGATG(9/13) 751 GGATG HapII 378 CCGG C^CGG 745 CCGG HpaII 378 CCGG C^CGG 745 CCGG MaeI 284 CTAG C^TAG 733 CTAG MspA1I 644 CAGCTG C[AC]G^C[GT]G 830 CAGCGG MspI 378 CCGG C^CGG 745 CCGG NlaIV 75 GGATCC GG[ACGT]^[ACGT]CC 294 GGAGCC NspI 143 ACATGC [GA]CATG^[CT] 841 GCATGT PleI 457 GACTC GAGTC(4/5) 264 GAGTC SmlI 848 CTCAAG C^T[CT][GA]AG 942 CTTGAG TfiI 51 GAATC G^A[AT]TC 277 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AcsI AluI ApoI AvaII BbvI BmyI BsaJI BsiYI BslI BsmI Bsp1286I BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII EcoRII EcoT14I Fnu4HI HaeIII HinfI HphI ItaI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII Sau3AI Sau96I ScrFI SfaNI StyI Tru9I TseI Tsp45I Tsp509I TspRI