(Circular) (Six-base) MAPSORT of: pFN21AE2475.seq Check: 4011 from: 1 to: 5797 pFN21AE2475 5797 bp With 182 enzymes: SgfI * May 14, 2013 11:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3607 278 Size: 53 83 186 936 2071 2468 Fragments arranged by size: 2468 2071 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3140 1937 Size: 1203 4594 Acc65I G'GTAC_C Cuts at: 3118 3118 Size: 5797 AclI AA'CG_TT Cuts at: 1563 3924 4297 1563 Size: 2361 373 3063 Fragments arranged by size: 3063 2361 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2154 2889 2936 3011 3030 3860 5244 5512 Size: 522 735 47 75 19 830 1384 268 Cuts at: 5512 1632 Size: 1917 Fragments arranged by size: 1917 1384 830 735 522 268 75 47 19 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5600 AflIII A'CryG_T Cuts at: 1203 4696 5673 1203 Size: 3493 977 1327 Fragments arranged by size: 3493 1327 977 AgeI A'CCGG_T Cuts at: 4628 4628 Size: 5797 AhdI GACnn_n'nnGTC Cuts at: 4526 4526 Size: 5797 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5797 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5797 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3062 3094 3062 Size: 32 5765 AlwNI CAG_nnn'CTG Cuts at: 3540 5112 3540 Size: 1572 4225 ApaLI G'TGCA_C Cuts at: 2436 3854 5010 2436 Size: 1418 1156 3223 Fragments arranged by size: 3223 1418 1156 ApoI r'AATT_y Cuts at: 1493 2047 2591 2952 3106 3332 3484 5593 1493 Size: 554 544 361 154 226 152 2109 1697 Fragments arranged by size: 2109 1697 554 544 361 226 154 152 AseI AT'TA_AT Cuts at: 160 1032 4351 160 Size: 872 3319 1606 Fragments arranged by size: 3319 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 AvaI C'yCGr_G Cuts at: 1870 1943 3122 1870 Size: 73 1179 4545 Fragments arranged by size: 4545 1179 73 AvrII C'CTAG_G Cuts at: 2737 2737 Size: 5797 BamHI G'GATC_C Cuts at: 2080 3127 3494 2080 Size: 1047 367 4383 Fragments arranged by size: 4383 1047 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3118 4573 618 Size: 325 217 627 1331 1455 1842 Fragments arranged by size: 1842 1455 1331 627 325 217 BanII G_rGCy'C Cuts at: 1391 2792 3116 1391 Size: 1401 324 4072 Fragments arranged by size: 4072 1401 324 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4826 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3988 4022 1903 Size: 34 2051 34 3678 Fragments arranged by size: 3678 2051 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3689 4899 3689 Size: 1210 4587 BclI T'GATC_A Cuts at: 1548 2175 1548 Size: 627 5170 BfrBI ATG'CAT Cuts at: 2699 2699 Size: 5797 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4408 136 Size: 107 122 71 631 605 2736 1525 Fragments arranged by size: 2736 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5792 5792 Size: 5797 BlpI GC'TnA_GC Cuts at: 3214 3214 Size: 5797 Bme1580I G_kGCm'C Cuts at: 1790 2440 3858 5014 1790 Size: 650 1418 1156 2573 Fragments arranged by size: 2573 1418 1156 650 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5797 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2334 3528 4486 Size: 416 53 83 399 24 917 1194 958 Cuts at: 4486 442 Size: 1753 Fragments arranged by size: 1753 1194 958 917 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5797 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4457 1239 Size: 3218 2579 Bpu10I CC'TnA_GC Cuts at: 2619 2619 Size: 5797 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2838 2968 3922 4787 5085 2838 Size: 130 954 865 298 3550 Fragments arranged by size: 3550 954 865 298 130 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4460 882 Size: 617 2961 2219 Fragments arranged by size: 2961 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3588 493 Size: 711 2384 2702 Fragments arranged by size: 2702 2384 711 BsaBI GATnn'nnATC Cuts at: 3040 5791 3040 Size: 2751 3046 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3604 3986 4621 275 Size: 53 83 186 936 2071 382 635 1451 Fragments arranged by size: 2071 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2749 4230 4628 4902 5049 5460 2749 Size: 1481 398 274 147 411 3086 Fragments arranged by size: 3086 1481 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5572 Fragments arranged by size: 5572 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2570 2752 1186 Size: 1384 182 4231 Fragments arranged by size: 4231 1384 182 BseYI C'CCAG_C Cuts at: 2791 5000 5496 2791 Size: 2209 496 3092 Fragments arranged by size: 3092 2209 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5224 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3247 4008 5036 1540 Size: 275 186 1246 761 1028 2301 Fragments arranged by size: 2301 1246 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2440 2854 3858 3943 5014 2440 Size: 414 1004 85 1071 3223 Fragments arranged by size: 3223 1071 1004 414 85 BsmI GAATG_Cn' Cuts at: 2209 3020 3056 3308 3401 2209 Size: 811 36 252 93 4605 Fragments arranged by size: 4605 811 252 93 36 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2440 2792 2854 3116 3858 3943 5014 Size: 399 650 352 62 262 742 85 1071 Cuts at: 5014 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 650 399 352 262 85 62 BspEI T'CCGG_A Cuts at: 2749 5460 2749 Size: 2711 3086 BspHI T'CATG_A Cuts at: 2422 3686 5416 2422 Size: 1264 1730 2803 Fragments arranged by size: 2803 1730 1264 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3152 844 Size: 2308 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3684 1394 Size: 429 1861 3507 Fragments arranged by size: 3507 1861 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2025 3025 4292 4466 65 Size: 1183 777 1000 1267 174 1396 Fragments arranged by size: 1396 1267 1183 1000 777 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2382 4441 4628 1700 Size: 110 572 2059 187 2869 Fragments arranged by size: 2869 2059 572 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5797 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5797 BssSI C'ACGA_G Cuts at: 3857 4869 3857 Size: 1012 4785 BstAPI GCAn_nnn'nTGC Cuts at: 794 2204 794 Size: 1410 4387 BstBI TT'CG_AA Cuts at: 4601 4601 Size: 5797 BstEII G'GTnAC_C Cuts at: 1184 2344 1184 Size: 1160 4637 BstYI r'GATC_y Cuts at: 1972 2080 3127 3494 3879 3896 5348 5434 5446 Size: 108 1047 367 385 17 1452 86 12 Cuts at: 5446 5792 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1047 385 367 346 108 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5245 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5058 Fragments arranged by size: 5058 683 56 BtsI GCAGTG_nn' Cuts at: 2640 3313 4107 4127 2640 Size: 673 794 20 4310 Fragments arranged by size: 4310 794 673 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5797 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3045 3080 529 Size: 35 2481 35 3246 Fragments arranged by size: 3246 2481 35 35 DraI TTT'AAA Cuts at: 3101 3101 Size: 5797 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4804 809 Size: 507 3488 1802 Fragments arranged by size: 3488 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3244 4133 5762 Size: 54 1425 50 275 18 1414 889 1629 Cuts at: 5762 8 Size: 43 Fragments arranged by size: 1629 1425 1414 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3244 1537 Size: 275 1432 4090 Fragments arranged by size: 4090 1432 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3727 1353 Size: 2374 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4384 4758 4904 387 Size: 1064 138 2795 374 146 1280 Fragments arranged by size: 2795 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2154 2889 2936 3011 3030 3860 4457 Size: 393 522 735 47 75 19 830 597 Cuts at: 4457 5244 5512 1239 Size: 787 268 1524 Fragments arranged by size: 1524 830 787 735 597 522 393 268 75 47 19 EcoNI CCTnn'n_nnAGG Cuts at: 2222 2222 Size: 5797 EcoO109I rG'GnC_Cy Cuts at: 2138 2138 Size: 5797 EcoRI G'AATT_C Cuts at: 3106 3484 3106 Size: 378 5419 FspI TGC'GCA Cuts at: 4303 4303 Size: 5797 HaeII r_GCGC'y Cuts at: 1678 4944 1678 Size: 3266 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2014 2046 2243 2275 2303 Size: 32 135 32 442 32 197 32 28 Cuts at: 2303 2335 4438 4470 4512 4544 1373 Size: 32 2103 32 42 32 2626 Fragments arranged by size: 2626 2103 442 197 135 42 32 32 32 32 32 32 32 28 HincII GTy'rAC Cuts at: 669 1657 1938 2832 3141 3387 669 Size: 988 281 894 309 246 3079 Fragments arranged by size: 3079 988 894 309 281 246 HindIII A'AGCT_T Cuts at: 748 2930 748 Size: 2182 3615 HpaI GTT'AAC Cuts at: 2832 3387 2832 Size: 555 5242 KpnI G_GTAC'C Cuts at: 3122 3122 Size: 5797 MfeI C'AATT_G Cuts at: 3396 3396 Size: 5797 MluI A'CGCG_T Cuts at: 5673 5673 Size: 5797 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4911 5095 1305 Size: 3606 184 2007 Fragments arranged by size: 3606 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3975 Fragments arranged by size: 3975 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2414 3755 4114 4273 518 Size: 648 1248 1341 359 159 2042 Fragments arranged by size: 2042 1341 1248 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2651 2837 3213 3890 5038 5283 1606 Size: 1045 186 376 677 1148 245 2120 Fragments arranged by size: 2120 1148 1045 677 376 245 186 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5245 NdeI CA'TA_TG Cuts at: 387 387 Size: 5797 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5797 NotI GC'GGCC_GC Cuts at: 3244 3244 Size: 5797 NsiI A_TGCA'T Cuts at: 2701 2701 Size: 5797 NspI r_CATG'y Cuts at: 1132 1229 2152 2850 3155 4700 1132 Size: 97 923 698 305 1545 2229 Fragments arranged by size: 2229 1545 923 698 305 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5797 PciI A'CATG_T Cuts at: 4696 4696 Size: 5797 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5797 PfoI T'CCnGG_A Cuts at: 2083 2083 Size: 5797 PmeI GTTT'AAAC Cuts at: 3101 3101 Size: 5797 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3866 3898 5379 5411 3866 Size: 32 1481 32 4252 Fragments arranged by size: 4252 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2138 2138 Size: 5797 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5797 PsiI TTA'TAA Cuts at: 3367 3367 Size: 5797 PstI C_TGCA'G Cuts at: 830 3149 830 Size: 2319 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5797 PvuII CAG'CTG Cuts at: 2651 2651 Size: 5797 SalI G'TCGA_C Cuts at: 1936 3139 1936 Size: 1203 4594 SbfI CC_TGCA'GG Cuts at: 3149 3149 Size: 5797 ScaI AGT'ACT Cuts at: 4045 5757 4045 Size: 1712 4085 SexAI A'CCwGG_T Cuts at: 2158 2158 Size: 5797 SfcI C'TryA_G Cuts at: 826 1045 3145 4280 4961 5152 826 Size: 219 2100 1135 681 191 1471 Fragments arranged by size: 2100 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5797 SmaI CCC'GGG Cuts at: 1872 3124 1872 Size: 1252 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2853 2947 3901 4802 5064 820 Size: 197 926 910 94 954 901 262 1553 Fragments arranged by size: 1553 954 926 910 901 262 197 94 SnaBI TAC'GTA Cuts at: 493 3588 493 Size: 3095 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5797 SphI G_CATG'C Cuts at: 1132 3155 1132 Size: 2023 3774 SspI AAT'ATT Cuts at: 5 52 3721 5 Size: 47 3669 2081 Fragments arranged by size: 3669 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5797 StyI C'CwwG_G Cuts at: 513 1065 2209 2223 2674 2737 2796 3236 513 Size: 552 1144 14 451 63 59 440 3074 Fragments arranged by size: 3074 1144 552 451 440 63 59 14 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3824 3841 3994 4179 4624 325 Size: 1255 2244 17 153 185 445 1498 Fragments arranged by size: 2244 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4043 5755 96 Size: 274 80 33 51 1443 2066 1712 138 Fragments arranged by size: 2066 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2079 2111 2521 2553 5757 5789 2079 Size: 32 410 32 3204 32 2087 Fragments arranged by size: 3204 2087 410 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5797 XbaI T'CTAG_A Cuts at: 3133 3133 Size: 5797 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5797 XmaI C'CCGG_G Cuts at: 1870 3122 1870 Size: 1252 4545 XmnI GAAnn'nnTTC Cuts at: 3926 3926 Size: 5797 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3605 276 Size: 53 83 186 936 2071 2468 Fragments arranged by size: 2468 2071 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI