(Linear) (Six-base) MAPSORT of: KIEE2475.seq Check: 9033 from: 1 to: 1092 KIEE2475.orf With 182 enzymes: SgfI * May 14, 2013 10:46 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 149 884 931 1006 1025 1092 Size: 149 735 47 75 19 67 Fragments arranged by size: 735 149 75 67 47 19 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1057 1089 1092 Size: 1057 32 3 Fragments arranged by size: 1057 32 3 ApaLI G'TGCA_C Cuts at: 0 431 1092 Size: 431 661 ApoI r'AATT_y Cuts at: 0 42 586 947 1092 Size: 42 544 361 145 Fragments arranged by size: 544 361 145 42 AvrII C'CTAG_G Cuts at: 0 732 1092 Size: 732 360 BamHI G'GATC_C Cuts at: 0 75 1092 Size: 75 1017 BanII G_rGCy'C Cuts at: 0 787 1092 Size: 787 305 BclI T'GATC_A Cuts at: 0 170 1092 Size: 170 922 BfrBI ATG'CAT Cuts at: 0 694 1092 Size: 694 398 Bme1580I G_kGCm'C Cuts at: 0 435 1092 Size: 435 657 BmrI ACTGGGnnnn_n' Cuts at: 0 329 1092 Size: 329 763 Bpu10I CC'TnA_GC Cuts at: 0 614 1092 Size: 614 478 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 833 963 1092 Size: 833 130 129 Fragments arranged by size: 833 130 129 BsaBI GATnn'nnATC Cuts at: 0 1035 1092 Size: 1035 57 BsaWI w'CCGG_w Cuts at: 0 744 1092 Size: 744 348 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 565 747 1092 Size: 565 182 345 Fragments arranged by size: 565 345 182 BseYI C'CCAG_C Cuts at: 0 786 1092 Size: 786 306 BsiHKAI G_wGCw'C Cuts at: 0 435 849 1092 Size: 435 414 243 Fragments arranged by size: 435 414 243 BsmI GAATG_Cn' Cuts at: 0 204 1015 1051 1092 Size: 204 811 36 41 Fragments arranged by size: 811 204 41 36 Bsp1286I G_dGCh'C Cuts at: 0 435 787 849 1092 Size: 435 352 62 243 Fragments arranged by size: 435 352 243 62 BspEI T'CCGG_A Cuts at: 0 744 1092 Size: 744 348 BspHI T'CATG_A Cuts at: 0 417 1092 Size: 417 675 BsrDI GCAATG_nn' Cuts at: 0 20 1020 1092 Size: 20 1000 72 Fragments arranged by size: 1000 72 20 BsrFI r'CCGG_y Cuts at: 0 377 1092 Size: 377 715 BstAPI GCAn_nnn'nTGC Cuts at: 0 199 1092 Size: 199 893 BstEII G'GTnAC_C Cuts at: 0 339 1092 Size: 339 753 BstYI r'GATC_y Cuts at: 0 75 1092 Size: 75 1017 BtsI GCAGTG_nn' Cuts at: 0 635 1092 Size: 635 457 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1040 1075 1092 Size: 1040 35 17 Fragments arranged by size: 1040 35 17 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 149 884 931 1006 1025 1092 Size: 149 735 47 75 19 67 Fragments arranged by size: 735 149 75 67 47 19 EcoNI CCTnn'n_nnAGG Cuts at: 0 217 1092 Size: 217 875 EcoO109I rG'GnC_Cy Cuts at: 0 133 1092 Size: 133 959 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 9 41 238 270 298 330 1092 Size: 9 32 197 32 28 32 762 Fragments arranged by size: 762 197 32 32 32 28 9 HincII GTy'rAC Cuts at: 0 827 1092 Size: 827 265 HindIII A'AGCT_T Cuts at: 0 925 1092 Size: 925 167 HpaI GTT'AAC Cuts at: 0 827 1092 Size: 827 265 MslI CAynn'nnrTG Cuts at: 0 409 1092 Size: 409 683 MspA1I CmG'CkG Cuts at: 0 646 832 1092 Size: 646 186 260 Fragments arranged by size: 646 260 186 NsiI A_TGCA'T Cuts at: 0 696 1092 Size: 696 396 NspI r_CATG'y Cuts at: 0 147 845 1092 Size: 147 698 247 Fragments arranged by size: 698 247 147 PfoI T'CCnGG_A Cuts at: 0 78 1092 Size: 78 1014 PpuMI rG'GwC_Cy Cuts at: 0 133 1092 Size: 133 959 PvuII CAG'CTG Cuts at: 0 646 1092 Size: 646 446 SexAI A'CCwGG_T Cuts at: 0 153 1092 Size: 153 939 SmlI C'TyrA_G Cuts at: 0 848 942 1092 Size: 848 94 150 Fragments arranged by size: 848 150 94 StyI C'CwwG_G Cuts at: 0 204 218 669 732 791 1092 Size: 204 14 451 63 59 301 Fragments arranged by size: 451 301 204 63 59 14 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 74 106 516 548 1092 Size: 74 32 410 32 544 Fragments arranged by size: 544 410 74 32 32 Enzymes that do cut: AcuI AloI ApaLI ApoI AvrII BamHI BanII BclI BfrBI Bme1580I BmrI Bpu10I BpuEI BsaBI BsaWI BseRI BseYI BsiHKAI BsmI Bsp1286I BspEI BspHI BsrDI BsrFI BstAPI BstEII BstYI BtsI CspCI Eco57MI EcoNI EcoO109I Hin4I HincII HindIII HpaI MslI MspA1I NsiI NspI PfoI PpuMI PvuII SexAI SmlI StyI TstI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI AscI AseI AsiSI AvaI BaeI BanI BbeI BbsI BbvCI BcgI BciVI BglI BglII BlpI BmgBI BmtI BplI BpmI BsaI BsaAI BsaHI BsaXI BsgI BsiEI BsiWI BsmBI BspMI BsrBI BsrGI BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtgI BtgZI ClaI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII KasI KpnI MfeI MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI