################################## # NO CUT ENZYMES ( 170 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspHI AspI AvaI AviII AvrII BalI BanII BbeI BbrPI BbsI BbvCI BcgI BfaI BfrI BglI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI EclXI Eco47III Eco52I Eco81I EcoT14I EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MslI MunI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TspRI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AciI 623 GCGG CCGC(-3/-1) AhdI 537 GACGGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 309 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG AspEI 537 GACGGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 187 GGATCC G^GATCC BciVI 527 GTATCC GTATCC(6/5) BcnI 361 CCCGG CC^[GC]GG BglII 507 AGATCT A^GATCT BseRI 895 CTCCTC GAGGAG(10/8) BsgI 215 GTGCAG GTGCAG(16/14) BsmAI 535 GAGAC GTCTC(1/5) BsmBI 535 GAGACG CGTCTC(1/5) BstEII 709 GGTTACC G^GT[ACGT]ACC BstPI 709 GGTTACC G^GT[ACGT]ACC DdeI 165 CTCAG C^T[ACGT]AG DraII 219 AGGTCCC [GA]G^G[ACGT]CC[CT] EaeI 929 TGGCCG [CT]^GGCC[GA] Eam1105I 537 GACGGTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 823 CCTATAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 219 AGGTCCC [GA]G^G[ACGT]CC[CT] EcoO65I 709 GGTTACC G^GT[ACGT]ACC EcoRI 753 GAATTC G^AATTC EcoRV 299 GATATC GAT^ATC HapII 362 CCGG C^CGG HinfI 72 GATTC G^A[ACGT]TC HpaII 362 CCGG C^CGG HphI 87 GGTGA GGTGA(8/7) MseI 584 TTAA T^TAA MspA1I 312 CAGCTG C[AC]G^C[GT]G MspI 362 CCGG C^CGG NciI 361 CCCGG CC^[GC]GG NdeI 885 CATATG CA^TATG NlaIII 195 CATG CATG^ PpuMI 219 AGGTCCC [GA]G^G[AT]CC[CT] PvuII 312 CAGCTG CAG^CTG SfaNI 626 GCATC GCATC(5/9) SmlI 532 CTTGAG C^T[CT][GA]AG TfiI 72 GATTC G^A[AT]TC Tru9I 584 TTAA T^TAA XcmI 852 CCAGACATACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcsI 496 AAATTC [GA]^AATT[CT] 753 GAATTC AfaI 733 GTAC GT^AC 922 GTAC AluI 313 AGCT AG^CT 444 AGCT AlwI 188 GATCC GGATC(4/5) 187 GGATC ApoI 496 AAATTC [GA]^AATT[CT] 753 GAATTC BanI 160 GGCACC G^G[CT][GA]CC 483 GGCACC BbvI 311 GCAGC GCAGC(8/12) 394 GCTGC BclI 305 TGATCA T^GATCA 570 TGATCA BsaJI 41 CCCTGG C^C[ACGT][ACGT]GG 224 CCCTGG BspMI 635 ACCTGC ACCTGC(4/8) 217 GCAGGT BstYI 187 GGATCC [GA]^GATC[CT] 507 AGATCT Cac8I 639 GCTTGC GC[ACGT]^[ACGT]GC 829 GCAGGC FbaI 305 TGATCA T^GATCA 570 TGATCA HaeIII 318 GGCC GG^CC 930 GGCC MflI 187 GGATCC [GA]^GATC[CT] 507 AGATCT RsaI 733 GTAC GT^AC 922 GTAC TseI 311 GCAGC G^C[AT]GC 394 GCTGC Tsp45I 242 GTGAC ^GT[GC]AC 343 GTGAC XhoII 187 GGATCC [GA]^GATC[CT] 507 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AvaII BsiYI BslI BsmFI BsrI BssKI BstF5I BstNI Cfr13I DpnII EarI EcoRII Fnu4HI FokI ItaI Ksp632I MaeIII MboI MboII MnlI MvaI MwoI NdeII NlaIV Sau3AI Sau96I ScrFI SfcI Tsp509I