(Circular) (Six-base) MAPSORT of: pFN21AE6381.seq Check: 9292 from: 1 to: 5662 pFN21AE6381 5662 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3472 278 Size: 53 83 186 936 1936 2468 Fragments arranged by size: 2468 1936 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3005 1937 Size: 1068 4594 Acc65I G'GTAC_C Cuts at: 2983 2983 Size: 5662 AclI AA'CG_TT Cuts at: 1563 3789 4162 1563 Size: 2226 373 3063 Fragments arranged by size: 3063 2226 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2030 2892 3725 5109 5377 1632 Size: 398 862 833 1384 268 1917 Fragments arranged by size: 1917 1384 862 833 398 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5465 AflIII A'CryG_T Cuts at: 1203 4561 5538 1203 Size: 3358 977 1327 Fragments arranged by size: 3358 1327 977 AgeI A'CCGG_T Cuts at: 4493 4493 Size: 5662 AhdI GACnn_n'nnGTC Cuts at: 2547 4391 2547 Size: 1844 3818 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5662 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5662 AlwNI CAG_nnn'CTG Cuts at: 2319 3405 4977 2319 Size: 1086 1572 3004 Fragments arranged by size: 3004 1572 1086 ApaLI G'TGCA_C Cuts at: 3719 4875 3719 Size: 1156 4506 ApoI r'AATT_y Cuts at: 1493 2501 2758 2971 3197 3349 5458 1493 Size: 1008 257 213 226 152 2109 1697 Fragments arranged by size: 2109 1697 1008 257 226 213 152 AseI AT'TA_AT Cuts at: 160 1032 4216 160 Size: 872 3184 1606 Fragments arranged by size: 3184 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5662 AvaI C'yCGr_G Cuts at: 1870 1943 2987 1870 Size: 73 1044 4545 Fragments arranged by size: 4545 1044 73 BamHI G'GATC_C Cuts at: 2192 2992 3359 2192 Size: 800 367 4495 Fragments arranged by size: 4495 800 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2165 2488 2983 4438 618 Size: 325 217 627 378 323 495 1455 1842 Fragments arranged by size: 1842 1455 627 495 378 325 323 217 BanII G_rGCy'C Cuts at: 1391 2981 1391 Size: 1590 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4691 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3853 3887 1903 Size: 34 50 34 1832 34 3678 Fragments arranged by size: 3678 1832 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2543 3554 4764 2543 Size: 1011 1210 3441 Fragments arranged by size: 3441 1210 1011 BclI T'GATC_A Cuts at: 1548 2310 2575 1548 Size: 762 265 4635 Fragments arranged by size: 4635 762 265 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4273 136 Size: 107 122 71 631 605 2601 1525 Fragments arranged by size: 2601 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2512 5657 2512 Size: 3145 2517 BlpI GC'TnA_GC Cuts at: 3079 3079 Size: 5662 Bme1580I G_kGCm'C Cuts at: 1790 3723 4879 1790 Size: 1933 1156 2573 Fragments arranged by size: 2573 1933 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5662 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3393 4351 442 Size: 416 53 83 399 24 1976 958 1753 Fragments arranged by size: 1976 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5662 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4322 1239 Size: 3083 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2558 3787 4652 4950 2558 Size: 1229 865 298 3270 Fragments arranged by size: 3270 1229 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4325 882 Size: 617 2826 2219 Fragments arranged by size: 2826 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3453 493 Size: 711 2249 2702 Fragments arranged by size: 2702 2249 711 BsaBI GATnn'nnATC Cuts at: 5656 5656 Size: 5662 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3469 3851 4486 275 Size: 53 83 186 936 1936 382 635 1451 Fragments arranged by size: 1936 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4095 4493 4767 4914 5325 4095 Size: 398 274 147 411 4432 Fragments arranged by size: 4432 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5437 Fragments arranged by size: 5437 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2891 1186 Size: 1705 3957 BseYI C'CCAG_C Cuts at: 2319 2801 4865 5361 2319 Size: 482 2064 496 2620 Fragments arranged by size: 2620 2064 496 482 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2241 1163 Size: 573 505 4584 Fragments arranged by size: 4584 573 505 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3112 3873 4901 1540 Size: 275 186 1111 761 1028 2301 Fragments arranged by size: 2301 1111 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3723 3808 4879 3723 Size: 85 1071 4506 Fragments arranged by size: 4506 1071 85 BsmI GAATG_Cn' Cuts at: 3173 3266 3173 Size: 93 5569 BsmBI CGTCTCn'nnnn_ Cuts at: 2534 2534 Size: 5662 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2981 3723 3808 4879 1391 Size: 399 1191 742 85 1071 2174 Fragments arranged by size: 2174 1191 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5325 5325 Size: 5662 BspHI T'CATG_A Cuts at: 3551 5281 3551 Size: 1730 3932 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2213 2649 3017 844 Size: 1369 436 368 3489 Fragments arranged by size: 3489 1369 436 368 BsrBI CCG'CTC Cuts at: 1394 1823 3549 1394 Size: 429 1726 3507 Fragments arranged by size: 3507 1726 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4157 4331 65 Size: 1183 2909 174 1396 Fragments arranged by size: 2909 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4306 4493 1700 Size: 110 2496 187 2869 Fragments arranged by size: 2869 2496 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5662 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5662 BssSI C'ACGA_G Cuts at: 3722 4734 3722 Size: 1012 4650 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5662 BstBI TT'CG_AA Cuts at: 4466 4466 Size: 5662 BstEII G'GTnAC_C Cuts at: 1184 2714 1184 Size: 1530 4132 BstYI r'GATC_y Cuts at: 1972 2192 2512 2992 3359 3744 3761 5213 5299 Size: 220 320 480 367 385 17 1452 86 Cuts at: 5299 5311 5657 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 480 385 367 346 320 220 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5110 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4923 Fragments arranged by size: 4923 683 56 BtsI GCAGTG_nn' Cuts at: 3178 3972 3992 3178 Size: 794 20 4848 Fragments arranged by size: 4848 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5662 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5627 DraI TTT'AAA Cuts at: 2966 2966 Size: 5662 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4669 809 Size: 507 3353 1802 Fragments arranged by size: 3353 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2934 3109 3998 Size: 54 1425 50 275 18 1104 175 889 Cuts at: 3998 5627 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1104 889 275 175 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3109 1537 Size: 275 1297 4090 Fragments arranged by size: 4090 1297 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2006 2148 2214 2911 3592 1353 Size: 653 142 66 697 681 3423 Fragments arranged by size: 3423 697 681 653 142 66 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4249 4623 4769 387 Size: 1064 138 2660 374 146 1280 Fragments arranged by size: 2660 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2030 2892 3725 4322 5109 5377 1239 Size: 393 398 862 833 597 787 268 1524 Fragments arranged by size: 1524 862 833 787 597 398 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2832 2832 Size: 5662 EcoO109I rG'GnC_Cy Cuts at: 2225 2225 Size: 5662 EcoRI G'AATT_C Cuts at: 2758 2971 3349 2758 Size: 213 378 5071 Fragments arranged by size: 5071 378 213 EcoRV GAT'ATC Cuts at: 2306 2306 Size: 5662 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2352 2352 Size: 5662 FspI TGC'GCA Cuts at: 4168 4168 Size: 5662 HaeII r_GCGC'y Cuts at: 1678 4809 1678 Size: 3131 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2295 2327 2533 2565 4303 Size: 32 135 32 723 32 206 32 1738 Cuts at: 4303 4335 4377 4409 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1738 723 206 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3006 3252 669 Size: 988 281 1068 246 3079 Fragments arranged by size: 3079 1068 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5662 HpaI GTT'AAC Cuts at: 3252 3252 Size: 5662 KpnI G_GTAC'C Cuts at: 2987 2987 Size: 5662 MfeI C'AATT_G Cuts at: 3261 3261 Size: 5662 MluI A'CGCG_T Cuts at: 5538 5538 Size: 5662 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2053 2165 4776 4960 1305 Size: 748 112 2611 184 2007 Fragments arranged by size: 2611 2007 748 184 112 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3840 Fragments arranged by size: 3840 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3620 3979 4138 518 Size: 648 2454 359 159 2042 Fragments arranged by size: 2454 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2319 3078 3755 4903 5148 1606 Size: 713 759 677 1148 245 2120 Fragments arranged by size: 2120 1148 759 713 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5110 NdeI CA'TA_TG Cuts at: 387 2891 387 Size: 2504 3158 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5662 NotI GC'GGCC_GC Cuts at: 3109 3109 Size: 5662 NspI r_CATG'y Cuts at: 1132 1229 3020 4565 1132 Size: 97 1791 1545 2229 Fragments arranged by size: 2229 1791 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5662 PciI A'CATG_T Cuts at: 4561 4561 Size: 5662 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5662 PmeI GTTT'AAAC Cuts at: 2966 2966 Size: 5662 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3731 3763 5244 5276 3731 Size: 32 1481 32 4117 Fragments arranged by size: 4117 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2225 2225 Size: 5662 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5662 PsiI TTA'TAA Cuts at: 3232 3232 Size: 5662 PstI C_TGCA'G Cuts at: 830 3014 830 Size: 2184 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5662 PvuII CAG'CTG Cuts at: 2319 2319 Size: 5662 SalI G'TCGA_C Cuts at: 1936 3004 1936 Size: 1068 4594 SbfI CC_TGCA'GG Cuts at: 3014 3014 Size: 5662 ScaI AGT'ACT Cuts at: 3910 5622 3910 Size: 1712 3950 SfcI C'TryA_G Cuts at: 826 1045 2445 2763 2829 3010 4145 4826 5017 Size: 219 1400 318 66 181 1135 681 191 Cuts at: 5017 826 Size: 1471 Fragments arranged by size: 1471 1400 1135 681 318 219 191 181 66 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5662 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5662 SmaI CCC'GGG Cuts at: 1872 2989 1872 Size: 1117 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2537 3766 4667 4929 820 Size: 197 926 594 1229 901 262 1553 Fragments arranged by size: 1553 1229 926 901 594 262 197 SnaBI TAC'GTA Cuts at: 493 3453 493 Size: 2960 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5662 SphI G_CATG'C Cuts at: 1132 3020 1132 Size: 1888 3774 SspI AAT'ATT Cuts at: 5 52 3586 5 Size: 47 3534 2081 Fragments arranged by size: 3534 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5662 StyI C'CwwG_G Cuts at: 513 1065 3101 513 Size: 552 2036 3074 Fragments arranged by size: 3074 2036 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3689 3706 3859 4044 4489 325 Size: 1255 2109 17 153 185 445 1498 Fragments arranged by size: 2109 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2737 3908 5620 Size: 274 80 33 51 1443 760 1171 1712 Cuts at: 5620 96 Size: 138 Fragments arranged by size: 1712 1443 1171 760 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2317 2349 2650 2682 5622 5654 2317 Size: 32 301 32 2940 32 2325 Fragments arranged by size: 2940 2325 301 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5662 XbaI T'CTAG_A Cuts at: 2998 2998 Size: 5662 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2864 2864 Size: 5662 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5662 XmaI C'CCGG_G Cuts at: 1870 2987 1870 Size: 1117 4545 XmnI GAAnn'nnTTC Cuts at: 3791 3791 Size: 5662 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3470 276 Size: 53 83 186 936 1936 2468 Fragments arranged by size: 2468 1936 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI