################################## # NO CUT ENZYMES ( 195 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaII AviII AvrII BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaI HphI KasI KpnI KspI MaeI MaeII MaeIII MamI MboI MfeI MflI MluI MroI MslI MspA1I MunI MvnI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AciI 25 CCGC CCGC(-3/-1) AflII 97 CTTAAG C^TTAAG AlwNI 56 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG AvaI 184 CCCGGG C^[CT]CG[GA]G BalI 138 TGGCCA TGG^CCA BanI 92 GGCACC G^G[CT][GA]CC BbvI 76 GCTGC GCAGC(8/12) BfrI 97 CTTAAG C^TTAAG BmyI 45 GGGCAC G[AGT]GC[ACT]^C BsiYI 43 CCGGGCACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 43 CCGGGCACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsoBI 184 CCCGGG C^[CT]CG[GA]G Bsp1286I 45 GGGCAC G[AGT]GC[ACT]^C BsrI 49 ACTGG ACTGG(1/-1) BstNI 224 CCTGG CC^[AT]GG Cac8I 27 GCCTGC GC[ACGT]^[ACGT]GC Cfr13I 182 GGCCC G^G[ACGT]CC DdeI 62 CTGAG C^T[ACGT]AG EaeI 138 TGGCCA [CT]^GGCC[GA] EarI 286 GAAGAG CTCTTC(1/4) EcoRII 224 CCTGG ^CC[AT]GG EcoT14I 248 CCAAGG C^C[AT][AT]GG FauI 24 CCCGC CCCGC(4/6) Fnu4HI 76 GCTGC GC^[ACGT]GC HindIII 266 AAGCTT A^AGCTT ItaI 76 GCTGC GC^[ACGT]GC Ksp632I 286 GAAGAG CTCTTC(1/4) MluNI 138 TGGCCA TGG^CCA MscI 138 TGGCCA TGG^CCA MseI 98 TTAA T^TAA MvaI 224 CCTGG CC^[AT]GG NlaIII 261 CATG CATG^ PstI 77 CTGCAG CTGCA^G SapI 286 GAAGAGC GCTCTTC(1/4) Sau96I 182 GGCCC G^G[ACGT]CC SfcI 77 CTGCAG C^T[GA][CT]AG SmaI 184 CCCGGG CCC^GGG SmlI 97 CTTAAG C^T[CT][GA]AG StyI 248 CCAAGG C^C[AT][AT]GG Tru9I 98 TTAA T^TAA TseI 76 GCTGC G^C[AT]GC TspRI 48 CACTG CA[GC]TG[ACGT][ACGT]^ XmaCI 184 CCCGGG C^CCGGG XmaI 184 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 8 enzymes) # ################################### BsaJI 184 CCCGGG C^C[ACGT][ACGT]GG 248 CCAAGG BsmFI 52 GGGAC GGGAC(10/14) 241 GGGAC HapII 43 CCGG C^CGG 185 CCGG HpaII 43 CCGG C^CGG 185 CCGG MboII 253 GAAGA GAAGA(8/7) 286 GAAGA MspI 43 CCGG C^CGG 185 CCGG MwoI 18 GCTCCTCCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 259 GCCATGAAAGC NlaIV 17 GGCTCC GG[ACGT]^[ACGT]CC 92 GGCACC ######################################### # 3 OR MORE CUT ENZYMES ( 11 enzymes) # ######################################### AluI BcnI BseRI BssKI BstF5I FokI HaeIII MnlI NciI ScrFI SfaNI