(Circular) (Six-base) MAPSORT of: pFN21AE6578.seq Check: 6176 from: 1 to: 5017 pFN21AE6578 5017 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2827 278 Size: 53 83 186 936 1291 2468 Fragments arranged by size: 2468 1291 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2360 1937 Size: 423 4594 Acc65I G'GTAC_C Cuts at: 2338 2338 Size: 5017 AclI AA'CG_TT Cuts at: 1563 3144 3517 1563 Size: 1581 373 3063 Fragments arranged by size: 3063 1581 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3080 4464 4732 1632 Size: 1448 1384 268 1917 Fragments arranged by size: 1917 1448 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2102 820 Size: 197 1085 3735 Fragments arranged by size: 3735 1085 197 AflIII A'CryG_T Cuts at: 1203 3916 4893 1203 Size: 2713 977 1327 Fragments arranged by size: 2713 1327 977 AgeI A'CCGG_T Cuts at: 3848 3848 Size: 5017 AhdI GACnn_n'nnGTC Cuts at: 3746 3746 Size: 5017 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5017 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5017 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2117 2149 2117 Size: 32 4985 AlwNI CAG_nnn'CTG Cuts at: 2066 2760 4332 2066 Size: 694 1572 2751 Fragments arranged by size: 2751 1572 694 ApaLI G'TGCA_C Cuts at: 3074 4230 3074 Size: 1156 3861 ApoI r'AATT_y Cuts at: 1493 2326 2552 2704 4813 1493 Size: 833 226 152 2109 1697 Fragments arranged by size: 2109 1697 833 226 152 AseI AT'TA_AT Cuts at: 160 1032 3571 160 Size: 872 2539 1606 Fragments arranged by size: 2539 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5017 AvaI C'yCGr_G Cuts at: 1870 1943 2189 2342 1870 Size: 73 246 153 4545 Fragments arranged by size: 4545 246 153 73 BamHI G'GATC_C Cuts at: 2347 2714 2347 Size: 367 4650 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2097 2338 3793 618 Size: 325 217 627 310 241 1455 1842 Fragments arranged by size: 1842 1455 627 325 310 241 217 BanII G_rGCy'C Cuts at: 1391 2336 1391 Size: 945 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4046 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3208 3242 1903 Size: 34 1271 34 3678 Fragments arranged by size: 3678 1271 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2909 4119 2909 Size: 1210 3807 BclI T'GATC_A Cuts at: 1548 1548 Size: 5017 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3628 136 Size: 107 122 71 631 605 1956 1525 Fragments arranged by size: 1956 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5012 5012 Size: 5017 BlpI GC'TnA_GC Cuts at: 2434 2434 Size: 5017 Bme1580I G_kGCm'C Cuts at: 1790 2054 3078 4234 1790 Size: 264 1024 1156 2573 Fragments arranged by size: 2573 1156 1024 264 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5017 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2064 2748 3706 Size: 416 53 83 399 24 647 684 958 Cuts at: 3706 442 Size: 1753 Fragments arranged by size: 1753 958 684 647 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5017 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3677 1239 Size: 2438 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3142 4007 4305 3142 Size: 865 298 3854 Fragments arranged by size: 3854 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3680 882 Size: 617 2181 2219 Fragments arranged by size: 2219 2181 617 BsaAI yAC'GTr Cuts at: 493 1204 2808 493 Size: 711 1604 2702 Fragments arranged by size: 2702 1604 711 BsaBI GATnn'nnATC Cuts at: 5011 5011 Size: 5017 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2824 3206 3841 275 Size: 53 83 186 936 1291 382 635 1451 Fragments arranged by size: 1451 1291 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3450 3848 4122 4269 4680 3450 Size: 398 274 147 411 3787 Fragments arranged by size: 3787 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2117 2147 1178 Size: 30 165 30 714 30 4048 Fragments arranged by size: 4048 714 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2015 2102 2138 1186 Size: 829 87 36 4065 Fragments arranged by size: 4065 829 87 36 BseYI C'CCAG_C Cuts at: 4220 4716 4220 Size: 496 4521 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4444 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2467 3228 4256 1540 Size: 275 186 466 761 1028 2301 Fragments arranged by size: 2301 1028 761 466 275 186 BsiHKAI G_wGCw'C Cuts at: 3078 3163 4234 3078 Size: 85 1071 3861 Fragments arranged by size: 3861 1071 85 BsmI GAATG_Cn' Cuts at: 2528 2621 2528 Size: 93 4924 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2336 3078 3163 4234 1391 Size: 399 264 282 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 282 264 85 BspEI T'CCGG_A Cuts at: 4680 4680 Size: 5017 BspHI T'CATG_A Cuts at: 2906 4636 2906 Size: 1730 3287 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2372 844 Size: 1528 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2904 1394 Size: 429 1081 3507 Fragments arranged by size: 3507 1081 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3512 3686 65 Size: 1183 2264 174 1396 Fragments arranged by size: 2264 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3661 3848 1700 Size: 110 1851 187 2869 Fragments arranged by size: 2869 1851 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5017 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5017 BssSI C'ACGA_G Cuts at: 3077 4089 3077 Size: 1012 4005 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5017 BstBI TT'CG_AA Cuts at: 3821 3821 Size: 5017 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5017 BstYI r'GATC_y Cuts at: 1972 2347 2714 3099 3116 4568 4654 4666 5012 Size: 375 367 385 17 1452 86 12 346 Cuts at: 5012 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 375 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4465 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4278 Fragments arranged by size: 4278 683 56 BtsI GCAGTG_nn' Cuts at: 2533 3327 3347 2533 Size: 794 20 4203 Fragments arranged by size: 4203 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5017 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4982 DraI TTT'AAA Cuts at: 2321 2321 Size: 5017 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4024 809 Size: 507 2708 1802 Fragments arranged by size: 2708 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2143 2464 3353 Size: 54 1425 50 275 18 313 321 889 Cuts at: 3353 4982 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 321 313 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2464 1537 Size: 275 652 4090 Fragments arranged by size: 4090 652 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2286 2947 1353 Size: 933 661 3423 Fragments arranged by size: 3423 933 661 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3604 3978 4124 387 Size: 1064 138 2015 374 146 1280 Fragments arranged by size: 2015 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3080 3677 4464 4732 1239 Size: 393 1448 597 787 268 1524 Fragments arranged by size: 1524 1448 787 597 393 268 EcoRI G'AATT_C Cuts at: 2326 2704 2326 Size: 378 4639 FspI TGC'GCA Cuts at: 3523 3523 Size: 5017 HaeII r_GCGC'y Cuts at: 1678 4164 1678 Size: 2486 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3658 3690 3732 3764 1373 Size: 32 135 32 2086 32 42 32 2626 Fragments arranged by size: 2626 2086 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2361 2607 669 Size: 988 281 423 246 3079 Fragments arranged by size: 3079 988 423 281 246 HindIII A'AGCT_T Cuts at: 748 2271 748 Size: 1523 3494 HpaI GTT'AAC Cuts at: 2607 2607 Size: 5017 KpnI G_GTAC'C Cuts at: 2342 2342 Size: 5017 MfeI C'AATT_G Cuts at: 2616 2616 Size: 5017 MluI A'CGCG_T Cuts at: 4893 4893 Size: 5017 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2178 4131 4315 1305 Size: 873 1953 184 2007 Fragments arranged by size: 2007 1953 873 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2145 10 Size: 54 1425 343 313 2882 Fragments arranged by size: 2882 1425 343 313 54 MslI CAynn'nnrTG Cuts at: 518 1166 2975 3334 3493 518 Size: 648 1809 359 159 2042 Fragments arranged by size: 2042 1809 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2433 3110 4258 4503 1606 Size: 827 677 1148 245 2120 Fragments arranged by size: 2120 1148 827 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4465 NdeI CA'TA_TG Cuts at: 387 387 Size: 5017 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5017 NotI GC'GGCC_GC Cuts at: 2464 2464 Size: 5017 NspI r_CATG'y Cuts at: 1132 1229 2375 3920 1132 Size: 97 1146 1545 2229 Fragments arranged by size: 2229 1545 1146 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5017 PciI A'CATG_T Cuts at: 3916 3916 Size: 5017 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5017 PmeI GTTT'AAAC Cuts at: 2321 2321 Size: 5017 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2117 2149 3086 3118 4599 4631 2117 Size: 32 937 32 1481 32 2503 Fragments arranged by size: 2503 1481 937 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5017 PsiI TTA'TAA Cuts at: 2587 2587 Size: 5017 PstI C_TGCA'G Cuts at: 830 2086 2369 830 Size: 1256 283 3478 Fragments arranged by size: 3478 1256 283 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5017 SalI G'TCGA_C Cuts at: 1936 2359 1936 Size: 423 4594 SapI GCTCTTCn'nnn_ Cuts at: 2286 2286 Size: 5017 SbfI CC_TGCA'GG Cuts at: 2369 2369 Size: 5017 ScaI AGT'ACT Cuts at: 3265 4977 3265 Size: 1712 3305 SfcI C'TryA_G Cuts at: 826 1045 2082 2365 3500 4181 4372 826 Size: 219 1037 283 1135 681 191 1471 Fragments arranged by size: 1471 1135 1037 681 283 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5017 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5017 SmaI CCC'GGG Cuts at: 1872 2191 2344 1872 Size: 319 153 4545 Fragments arranged by size: 4545 319 153 SmlI C'TyrA_G Cuts at: 820 1017 1943 2102 3121 4022 4284 820 Size: 197 926 159 1019 901 262 1553 Fragments arranged by size: 1553 1019 926 901 262 197 159 SnaBI TAC'GTA Cuts at: 493 2808 493 Size: 2315 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5017 SphI G_CATG'C Cuts at: 1132 2375 1132 Size: 1243 3774 SspI AAT'ATT Cuts at: 5 52 2941 5 Size: 47 2889 2081 Fragments arranged by size: 2889 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5017 StyI C'CwwG_G Cuts at: 513 1065 2253 2456 513 Size: 552 1188 203 3074 Fragments arranged by size: 3074 1188 552 203 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3044 3061 3214 3399 3844 325 Size: 1255 1464 17 153 185 445 1498 Fragments arranged by size: 1498 1464 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3263 4975 96 Size: 274 80 33 51 1443 1286 1712 138 Fragments arranged by size: 1712 1443 1286 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4977 5009 4977 Size: 32 4985 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5017 XbaI T'CTAG_A Cuts at: 2353 2353 Size: 5017 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5017 XmaI C'CCGG_G Cuts at: 1870 2189 2342 1870 Size: 319 153 4545 Fragments arranged by size: 4545 319 153 XmnI GAAnn'nnTTC Cuts at: 3146 3146 Size: 5017 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2825 276 Size: 53 83 186 936 1291 2468 Fragments arranged by size: 2468 1291 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI