################################## # NO CUT ENZYMES ( 196 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AfaI AflII AflIII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeIII HapII HgaI Hin1I HincII HindII HindIII HinfI HpaI HpaII KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MluNI MroI MscI MseI MspI MunI MwoI NaeI NarI NciI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccII 8 CGCG CG^CG AciI 15 GCGG CCGC(-3/-1) AcsI 286 GAATTC [GA]^AATT[CT] AhdI 254 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 294 GATCC GGATC(4/5) ApoI 286 GAATTC [GA]^AATT[CT] AspEI 254 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbvCI 74 CCTCAGC CCTCAGC(-5/-2) BpmI 318 CTCCAG CTGGAG(16/14) BsiYI 291 CCAGATCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 291 CCAGATCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmI 325 GCATTC GAATGC(1/-1) BsrI 320 CCAGT ACTGG(1/-1) BstUI 8 CGCG CG^CG BstXI 38 CCATGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 293 AGATCC [GA]^GATC[CT] DdeI 75 CTCAG C^T[ACGT]AG DpnII 294 GATC ^GATC Eam1105I 254 GACAGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 286 GAATTC G^AATTC HaeII 48 GGCGCT [GA]GCGC^[CT] HphI 329 TCACC GGTGA(8/7) MaeII 68 ACGT A^CGT MaeIII 34 GTGAC ^GT[ACGT]AC MboI 294 GATC ^GATC MboII 283 GAAGA GAAGA(8/7) MflI 293 AGATCC [GA]^GATC[CT] MslI 39 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 127 CAGCTG C[AC]G^C[GT]G MvnI 8 CGCG CG^CG NdeII 294 GATC ^GATC PflMI 291 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 127 CAGCTG CAG^CTG Sau3AI 294 GATC ^GATC Tsp45I 34 GTGAC ^GT[GC]AC Van91I 291 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 293 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AluI 128 AGCT AG^CT 276 AGCT AlwNI 77 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG 292 CAGATCCTG BbvI 4 GCAGC GCAGC(8/12) 51 GCTGC BsaJI 38 CCATGG C^C[ACGT][ACGT]GG 350 CCATGG BsmAI 173 GAGAC GTCTC(1/5) 28 GTCTC BssKI 297 CCTGG ^CC[ACGT]GG 308 CCTGG BstNI 297 CCTGG CC^[AT]GG 308 CCTGG Cac8I 269 GCAAGC GC[ACGT]^[ACGT]GC 273 GCAAGC CfoI 7 GCGC GCG^C 49 GCGC DsaI 38 CCATGG C^C[GA][CT]GG 350 CCATGG EcoRII 297 CCTGG ^CC[AT]GG 308 CCTGG EcoT14I 38 CCATGG C^C[AT][AT]GG 350 CCATGG Fnu4HI 4 GCAGC GC^[ACGT]GC 51 GCTGC HhaI 7 GCGC GCG^C 49 GCGC HinP1I 7 GCGC G^CGC 49 GCGC ItaI 4 GCAGC GC^[ACGT]GC 51 GCTGC MnlI 74 CCTC CCTC(7/6) 80 CCTC MvaI 297 CCTGG CC^[AT]GG 308 CCTGG NcoI 38 CCATGG C^CATGG 350 CCATGG ScrFI 297 CCTGG CC^[ACGT]GG 308 CCTGG StyI 38 CCATGG C^C[AT][AT]GG 350 CCATGG TseI 4 GCAGC G^C[AT]GC 51 GCTGC Tsp509I 211 AATT ^AATT 287 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 2 enzymes) # ######################################### NlaIII TspRI