################################## # NO CUT ENZYMES ( 205 enzymes) # ################################## AatII Acc65I AccI AccIII AciI AclI AcsI AfaI AflII AflIII AgeI AhdI AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BpmI BpuAI BsaAI BsaBI BsaI BsaJI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I Cac8I Cfr10I Cfr13I ClaI CpoI DpnII DraII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FokI FseI FspI HaeIII HapII HincII HindII HindIII HinfI HpaI HpaII ItaI KpnI KspI MaeI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MspA1I MspI MunI NaeI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI SexAI SfaNI SfcI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI TseI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccII 154 CGCG CG^CG AcyI 116 GGCGCC G[GA]^CG[CT]C BanI 116 GGCGCC G^G[CT][GA]CC BanII 204 GGGCTC G[GA]GC[CT]^C BbeI 116 GGCGCC GGCGC^C BlpI 174 GCTGAGC GC^T[ACGT]AGC BmyI 204 GGGCTC G[AGT]GC[ACT]^C Bpu1102I 174 GCTGAGC GC^T[ACGT]AGC BsaHI 116 GGCGCC G[GA]^CG[CT]C BseRI 7 GAGGAG GAGGAG(10/8) BsgI 124 CTGCAC GTGCAG(16/14) BsiYI 96 CCATGAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 96 CCATGAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 40 GTCTC GTCTC(1/5) Bsp1286I 204 GGGCTC G[AGT]GC[ACT]^C BsrDI 143 CATTGC GCAATG(2/0) BssKI 31 CCTGG ^CC[ACGT]GG BstNI 31 CCTGG CC^[AT]GG BstUI 154 CGCG CG^CG CelII 174 GCTGAGC GC^T[ACGT]AGC DraI 49 TTTAAA TTT^AAA DraIII 127 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 4 GAAGAG CTCTTC(1/4) EcoRII 31 CCTGG ^CC[AT]GG HaeII 116 GGCGCC [GA]GCGC^[CT] HgaI 155 GCGTC GACGC(5/10) Hin1I 116 GGCGCC G[GA]^CG[CT]C KasI 116 GGCGCC G^GCGCC Ksp632I 4 GAAGAG CTCTTC(1/4) MaeIII 106 GTGAC ^GT[ACGT]AC MboII 4 GAAGA GAAGA(8/7) MseI 50 TTAA T^TAA MslI 129 CACTGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvaI 31 CCTGG CC^[AT]GG MvnI 154 CGCG CG^CG NarI 116 GGCGCC GG^CGCC NlaIII 97 CATG CATG^ ScrFI 31 CCTGG CC^[ACGT]GG SfoI 116 GGCGCC GGC^GCC TaqI 158 TCGA T^CGA Tru9I 50 TTAA T^TAA Tsp45I 106 GTGAC ^GT[GC]AC Tsp509I 36 AATT ^AATT TspRI 129 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 158 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 6 enzymes) # ################################### CfoI 117 GCGC GCG^C 153 GCGC DdeI 149 CTGAG C^T[ACGT]AG 175 CTGAG HhaI 117 GCGC GCG^C 153 GCGC HinP1I 117 GCGC G^CGC 153 GCGC HphI 70 GGTGA GGTGA(8/7) 105 GGTGA NlaIV 116 GGCGCC GG[ACGT]^[ACGT]CC 205 GGCTCC ######################################### # 3 OR MORE CUT ENZYMES ( 2 enzymes) # ######################################### MnlI MwoI