################################## # NO CUT ENZYMES ( 187 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MluI MroI MslI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AlwI 225 GATCC GGATC(4/5) AspHI 36 GTGCTC G[AT]GC[AT]^C BalI 310 TGGCCA TGG^CCA BcnI 145 CCGGG CC^[GC]GG BsiHKAI 36 GTGCTC G[AT]GC[AT]^C BsmFI 147 GGGAC GGGAC(10/14) BspHI 23 TCATGA T^CATGA BsrFI 213 GCCGGT [GA]^CCGG[CT] BstAPI 299 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 191 GGATG GGATG(2/0) BstYI 224 AGATCC [GA]^GATC[CT] Cac8I 308 GCTGGC GC[ACGT]^[ACGT]GC Cfr10I 213 GCCGGT [GA]^CCGG[CT] DdeI 221 CTCAG C^T[ACGT]AG DpnII 225 GATC ^GATC DraII 289 AGGCCCT [GA]G^G[ACGT]CC[CT] DsaI 110 CCATGG C^C[GA][CT]GG EaeI 310 TGGCCA [CT]^GGCC[GA] EcoO109I 289 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoT14I 110 CCATGG C^C[AT][AT]GG FokI 191 GGATG GGATG(9/13) HinfI 149 GACTC G^A[ACGT]TC MaeIII 100 GTCAC ^GT[ACGT]AC MboI 225 GATC ^GATC MboII 57 GAAGA GAAGA(8/7) MflI 224 AGATCC [GA]^GATC[CT] MluNI 310 TGGCCA TGG^CCA MscI 310 TGGCCA TGG^CCA MspA1I 303 CAGCTG C[AC]G^C[GT]G NciI 145 CCGGG CC^[GC]GG NcoI 110 CCATGG C^CATGG NdeII 225 GATC ^GATC PleI 149 GACTC GAGTC(4/5) PstI 294 CTGCAG CTGCA^G PvuII 303 CAGCTG CAG^CTG RcaI 23 TCATGA T^CATGA Sau3AI 225 GATC ^GATC SfcI 294 CTGCAG C^T[GA][CT]AG StyI 110 CCATGG C^C[AT][AT]GG Tsp45I 100 GTCAC ^GT[GC]AC XhoII 224 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### BanI 91 GGTGCC G^G[CT][GA]CC 184 GGTGCC BmyI 36 GTGCTC G[AGT]GC[ACT]^C 92 GTGCCC BsaJI 95 CCCTGG C^C[ACGT][ACGT]GG 110 CCATGG Bsp1286I 36 GTGCTC G[AGT]GC[ACT]^C 92 GTGCCC BstNI 96 CCTGG CC^[AT]GG 188 CCTGG EcoRII 96 CCTGG ^CC[AT]GG 188 CCTGG HapII 145 CCGG C^CGG 214 CCGG HpaII 145 CCGG C^CGG 214 CCGG MseI 209 TTAA T^TAA 266 TTAA MspI 145 CCGG C^CGG 214 CCGG MvaI 96 CCTGG CC^[AT]GG 188 CCTGG Tru9I 209 TTAA T^TAA 266 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AciI AluI AlwNI BbvI BsiYI BslI BssKI Cfr13I Fnu4HI HaeIII ItaI MnlI MwoI NlaIII NlaIV Sau96I ScrFI TseI