################################## # NO CUT ENZYMES ( 204 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AfaI AflII AgeI AhdI AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII Bst1107I BstAPI BstBI BstEII BstNI BstPI BstXI BstYI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI Eam1105I Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HinfI HpaI HphI KasI KpnI KspI MaeIII MamI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI MvaI MwoI NaeI NarI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TaqI TfiI Tsp45I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### AccII 12 CGCG CG^CG AcsI 205 AAATTT [GA]^AATT[CT] AflIII 151 ACACGT A^C[GA][CT]GT ApoI 205 AAATTT [GA]^AATT[CT] BbvI 71 GCAGC GCAGC(8/12) BfaI 52 CTAG C^TAG BsaJI 56 CCCCGG C^C[ACGT][ACGT]GG BsiEI 8 CGGCCG CG[GA][CT]^CG BsiYI 51 CCTAGCCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 51 CCTAGCCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BssSI 91 CACGAG CACGAG(-5/-1) BstUI 12 CGCG CG^CG DpnII 160 GATC ^GATC EaeI 8 CGGCCG [CT]^GGCC[GA] EagI 8 CGGCCG C^GGCCG EarI 176 GAAGAG CTCTTC(1/4) EclXI 8 CGGCCG C^GGCCG Eco52I 8 CGGCCG C^GGCCG Ksp632I 176 GAAGAG CTCTTC(1/4) MaeI 52 CTAG C^TAG MboI 160 GATC ^GATC MseI 31 TTAA T^TAA MvnI 12 CGCG CG^CG NdeII 160 GATC ^GATC Sau3AI 160 GATC ^GATC SspI 107 AATATT AAT^ATT Tru9I 31 TTAA T^TAA TseI 71 GCAGC G^C[AT]GC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AvaII 42 GGACC G^G[AT]CC 87 GGACC BcnI 39 CCGGG CC^[GC]GG 57 CCCGG BseRI 179 GAGGAG GAGGAG(10/8) 232 GAGGAG BsmFI 41 GGGAC GGGAC(10/14) 86 GGGAC BssKI 39 CCGGG ^CC[ACGT]GG 57 CCCGG BstF5I 24 GGATG GGATG(2/0) 227 GGATG CfoI 13 GCGC GCG^C 69 GCGC Fnu4HI 10 GCCGC GC^[ACGT]GC 71 GCAGC FokI 24 GGATG GGATG(9/13) 227 GGATG HaeIII 9 GGCC GG^CC 37 GGCC HapII 39 CCGG C^CGG 58 CCGG HhaI 13 GCGC GCG^C 69 GCGC HinP1I 13 GCGC G^CGC 69 GCGC HpaII 39 CCGG C^CGG 58 CCGG ItaI 10 GCCGC GC^[ACGT]GC 71 GCAGC MaeII 77 ACGT A^CGT 153 ACGT MboII 176 GAAGA GAAGA(8/7) 214 GAAGA MnlI 179 GAGG CCTC(7/6) 232 GAGG MspI 39 CCGG C^CGG 58 CCGG NciI 39 CCGGG CC^[GC]GG 57 CCCGG NlaIV 41 GGGACC GG[ACGT]^[ACGT]CC 86 GGGACC ScrFI 39 CCGGG CC^[ACGT]GG 57 CCCGG Tsp509I 137 AATT ^AATT 206 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 3 enzymes) # ######################################### AciI Cfr13I Sau96I