Restriction map for
pFN21AE5560
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AflIII AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DrdI EaeI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII Hin1I
HpaI KasI KpnI KspI MaeII
MamI MfeI MluI MluNI MscI
MslI MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SphI SspBI SspI
StuI StyI SwaI Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 155 GTCGAC GT^[AC][GT]AC
AccII 474 CGCG CG^CG
AccIII 435 TCCGGA T^CCGGA
AhdI 424 GACGCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 424 GACGCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 248 GAGCTC G[AT]GC[AT]^C
AspI 290 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 412 GAAGAC GAAGAC(2/6)
BpuAI 412 GAAGAC GAAGAC(2/6)
BsaWI 435 TCCGGA [AT]^CCGG[AT]
BseAI 435 TCCGGA T^CCGGA
BseRI 581 CTCCTC GAGGAG(10/8)
BsgI 914 CTGCAC GTGCAG(16/14)
BsiHKAI 248 GAGCTC G[AT]GC[AT]^C
BsmAI 432 GTCTC GTCTC(1/5)
BspEI 435 TCCGGA T^CCGGA
BstAPI 716 GCAAAAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 474 CGCG CG^CG
CfoI 535 GCGC GCG^C
Eam1105I 424 GACGCTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 72 GAAGAG CTCTTC(1/4)
EcoRI 568 GAATTC G^AATTC
FauI 827 CCCGC CCCGC(4/6)
HapII 436 CCGG C^CGG
HhaI 535 GCGC GCG^C
HinP1I 535 GCGC G^CGC
HincII 155 GTCGAC GT[CT]^[GA]AC
HindII 155 GTCGAC GT[CT]^[GA]AC
HpaII 436 CCGG C^CGG
Ksp632I 72 GAAGAG CTCTTC(1/4)
MroI 435 TCCGGA T^CCGGA
MspI 436 CCGG C^CGG
MvnI 474 CGCG CG^CG
PflFI 290 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 248 GAGCTC GAGCT^C
SalI 155 GTCGAC G^TCGAC
SapI 72 GAAGAGC GCTCTTC(1/4)
SbfI 219 CCTGCAGG CCTGCA^GG
ScaI 1081 AGTACT AGT^ACT
SexAI 291 ACCTGGT A^CC[AT]GGT
SpeI 42 ACTAGT A^CTAGT
Sse8387I 219 CCTGCAGG CCTGCA^GG
Tth111I 290 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 568 GAATTC [GA]^AATT[CT]
1009 GAATTT
AfaI 759 GTAC GT^AC
1082 GTAC
AlwI 730 GATCC GGATC(4/5)
867 GATCC
ApoI 568 GAATTC [GA]^AATT[CT]
1009 GAATTT
BanII 75 GAGCCC G[GA]GC[CT]^C
248 GAGCTC
BbvCI 647 CCTCAGC CCTCAGC(-5/-2)
9 GCTGAGG
BmyI 75 GAGCCC G[AGT]GC[ACT]^C
248 GAGCTC
BsmFI 477 GTCCC GGGAC(10/14)
552 GTCCC
BsmI 85 GCATTC GAATGC(1/-1)
971 GCATTC
Bsp1286I 75 GAGCCC G[AGT]GC[ACT]^C
248 GAGCTC
BstYI 729 AGATCC [GA]^GATC[CT]
866 AGATCC
DraIII 934 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
952 CACATGGTG
DsaI 607 CCACGG C^C[GA][CT]GG
739 CCACGG
HgaI 424 GACGC GACGC(5/10)
475 GCGTC
HindIII 105 AAGCTT A^AGCTT
926 AAGCTT
MflI 729 AGATCC [GA]^GATC[CT]
866 AGATCC
MseI 344 TTAA T^TAA
754 TTAA
PvuII 7 CAGCTG CAG^CTG
847 CAGCTG
RsaI 759 GTAC GT^AC
1082 GTAC
TaqI 156 TCGA T^CGA
252 TCGA
Tru9I 344 TTAA T^TAA
754 TTAA
TthHB8I 156 TCGA T^CGA
252 TCGA
XhoII 729 AGATCC [GA]^GATC[CT]
866 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AlwNI AvaII BbvI
BfaI BpmI BsaJI BsiYI BslI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MaeIII MboI MboII MnlI
MspA1I MvaI MwoI NdeII NlaIII
PleI PstI Sau3AI Sau96I ScrFI
SfaNI SfcI TfiI TseI Tsp45I
Tsp509I TspRI