(Circular) (Six-base) MAPSORT of: pFN21AE5560.seq Check: 3689 from: 1 to: 5839 pFN21AE5560 5839 bp With 182 enzymes: SgfI * January 30, 2014 17:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3649 278 Size: 53 83 186 936 2113 2468 Fragments arranged by size: 2468 2113 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2161 3182 1937 Size: 224 1021 4594 Fragments arranged by size: 4594 1021 224 Acc65I G'GTAC_C Cuts at: 3160 3160 Size: 5839 AclI AA'CG_TT Cuts at: 1563 3966 4339 1563 Size: 2403 373 3063 Fragments arranged by size: 3063 2403 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2535 2970 3902 5286 5554 1632 Size: 903 435 932 1384 268 1917 Fragments arranged by size: 1917 1384 932 903 435 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5642 AflIII A'CryG_T Cuts at: 1203 4738 5715 1203 Size: 3535 977 1327 Fragments arranged by size: 3535 1327 977 AgeI A'CCGG_T Cuts at: 4670 4670 Size: 5839 AhdI GACnn_n'nnGTC Cuts at: 2434 4568 2434 Size: 2134 3705 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5839 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5839 AlwNI CAG_nnn'CTG Cuts at: 2570 2627 2854 2875 2948 2992 3582 5154 2570 Size: 57 227 21 73 44 590 1572 3255 Fragments arranged by size: 3255 1572 590 227 73 57 44 21 ApaLI G'TGCA_C Cuts at: 3896 5052 3896 Size: 1156 4683 ApoI r'AATT_y Cuts at: 1493 2573 3014 3148 3374 3526 5635 1493 Size: 1080 441 134 226 152 2109 1697 Fragments arranged by size: 2109 1697 1080 441 226 152 134 AseI AT'TA_AT Cuts at: 160 1032 4393 160 Size: 872 3361 1606 Fragments arranged by size: 3361 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5839 AvaI C'yCGr_G Cuts at: 1870 1943 3164 1870 Size: 73 1221 4545 Fragments arranged by size: 4545 1221 73 BamHI G'GATC_C Cuts at: 3169 3536 3169 Size: 367 5472 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3160 4615 618 Size: 325 217 627 1373 1455 1842 Fragments arranged by size: 1842 1455 1373 627 325 217 BanII G_rGCy'C Cuts at: 1391 2084 2257 3158 1391 Size: 693 173 901 4072 Fragments arranged by size: 4072 901 693 173 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2424 928 Size: 971 525 4343 Fragments arranged by size: 4343 971 525 BbvCI CC'TCA_GC Cuts at: 2015 2653 2015 Size: 638 5201 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4030 4064 1903 Size: 34 50 34 2009 34 3678 Fragments arranged by size: 3678 2009 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3731 4941 3731 Size: 1210 4629 BclI T'GATC_A Cuts at: 1548 1548 Size: 5839 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4450 136 Size: 107 122 71 631 605 2778 1525 Fragments arranged by size: 2778 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5834 5834 Size: 5839 BlpI GC'TnA_GC Cuts at: 3256 3256 Size: 5839 Bme1580I G_kGCm'C Cuts at: 1790 3900 5056 1790 Size: 2110 1156 2573 Fragments arranged by size: 2573 2110 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5839 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3570 4528 442 Size: 416 53 83 399 24 2153 958 1753 Fragments arranged by size: 2153 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5839 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1994 2326 2520 2825 4499 1239 Size: 755 332 194 305 1674 2579 Fragments arranged by size: 2579 1674 755 332 305 194 Bpu10I CC'TnA_GC Cuts at: 2015 2653 2015 Size: 638 5201 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3964 4829 5127 3964 Size: 865 298 4676 Fragments arranged by size: 4676 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4502 882 Size: 617 3003 2219 Fragments arranged by size: 3003 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3630 493 Size: 711 2426 2702 Fragments arranged by size: 2702 2426 711 BsaBI GATnn'nnATC Cuts at: 5833 5833 Size: 5839 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3646 4028 4663 275 Size: 53 83 186 936 2113 382 635 1451 Fragments arranged by size: 2113 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2440 4272 4670 4944 5091 5502 2440 Size: 1832 398 274 147 411 2777 Fragments arranged by size: 2777 1832 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2518 2548 1178 Size: 30 165 30 1115 30 4469 Fragments arranged by size: 4469 1115 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2577 1186 Size: 1391 4448 BseYI C'CCAG_C Cuts at: 2083 2668 5042 5538 2083 Size: 585 2374 496 2384 Fragments arranged by size: 2384 2374 585 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2904 1163 Size: 573 1168 4098 Fragments arranged by size: 4098 1168 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3289 4050 5078 1540 Size: 275 186 1288 761 1028 2301 Fragments arranged by size: 2301 1288 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2257 3900 3985 5056 2257 Size: 1643 85 1071 3040 Fragments arranged by size: 3040 1643 1071 85 BsmI GAATG_Cn' Cuts at: 2090 2976 3350 3443 2090 Size: 886 374 93 4486 Fragments arranged by size: 4486 886 374 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2084 2257 3158 3900 3985 5056 1391 Size: 399 294 173 901 742 85 1071 2174 Fragments arranged by size: 2174 1071 901 742 399 294 173 85 BspEI T'CCGG_A Cuts at: 2440 5502 2440 Size: 3062 2777 BspHI T'CATG_A Cuts at: 3728 5458 3728 Size: 1730 4109 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3194 844 Size: 2350 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3726 1394 Size: 429 1903 3507 Fragments arranged by size: 3507 1903 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4334 4508 65 Size: 1183 3086 174 1396 Fragments arranged by size: 3086 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4483 4670 1700 Size: 110 2673 187 2869 Fragments arranged by size: 2869 2673 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5839 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5839 BssSI C'ACGA_G Cuts at: 3899 4911 3899 Size: 1012 4827 BstAPI GCAn_nnn'nTGC Cuts at: 794 2727 794 Size: 1933 3906 BstBI TT'CG_AA Cuts at: 4643 4643 Size: 5839 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5839 BstYI r'GATC_y Cuts at: 1972 2734 2871 3169 3536 3921 3938 5390 5476 Size: 762 137 298 367 385 17 1452 86 Cuts at: 5476 5488 5834 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 762 385 367 346 298 137 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2612 2744 513 Size: 552 1547 132 3608 Fragments arranged by size: 3608 1547 552 132 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5100 Fragments arranged by size: 5100 683 56 BtsI GCAGTG_nn' Cuts at: 2431 3355 4149 4169 2431 Size: 924 794 20 4101 Fragments arranged by size: 4101 924 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5839 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5804 DraI TTT'AAA Cuts at: 3143 3143 Size: 5839 DraIII CAC_nnn'GTG Cuts at: 2944 2962 2944 Size: 18 5821 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4846 809 Size: 507 3530 1802 Fragments arranged by size: 3530 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3286 4175 5804 Size: 54 1425 50 275 18 1456 889 1629 Cuts at: 5804 8 Size: 43 Fragments arranged by size: 1629 1456 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3286 1537 Size: 275 1474 4090 Fragments arranged by size: 4090 1474 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2072 3769 1353 Size: 719 1697 3423 Fragments arranged by size: 3423 1697 719 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4426 4800 4946 387 Size: 1064 138 2837 374 146 1280 Fragments arranged by size: 2837 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2255 2255 Size: 5839 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1994 2326 2520 2535 2825 2970 3902 Size: 393 362 332 194 15 290 145 932 Cuts at: 3902 4499 5286 5554 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 932 787 597 393 362 332 290 268 194 145 15 EcoRI G'AATT_C Cuts at: 2573 3148 3526 2573 Size: 575 378 4886 Fragments arranged by size: 4886 575 378 FspI TGC'GCA Cuts at: 4345 4345 Size: 5839 HaeII r_GCGC'y Cuts at: 1678 4986 1678 Size: 3308 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2143 2175 2420 2452 2863 Size: 32 135 32 571 32 245 32 411 Cuts at: 2863 2895 4480 4512 4554 4586 1373 Size: 32 1585 32 42 32 2626 Fragments arranged by size: 2626 1585 571 411 245 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2162 3183 3429 669 Size: 988 281 224 1021 246 3079 Fragments arranged by size: 3079 1021 988 281 246 224 HindIII A'AGCT_T Cuts at: 748 2110 2931 748 Size: 1362 821 3656 Fragments arranged by size: 3656 1362 821 HpaI GTT'AAC Cuts at: 3429 3429 Size: 5839 KpnI G_GTAC'C Cuts at: 3164 3164 Size: 5839 MfeI C'AATT_G Cuts at: 3438 3438 Size: 5839 MluI A'CGCG_T Cuts at: 5715 5715 Size: 5839 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2615 4953 5137 1305 Size: 1310 2338 184 2007 Fragments arranged by size: 2338 2007 1310 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4017 Fragments arranged by size: 4017 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3797 4156 4315 518 Size: 648 2631 359 159 2042 Fragments arranged by size: 2631 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2014 2087 2854 3255 3932 5080 5325 1606 Size: 408 73 767 401 677 1148 245 2120 Fragments arranged by size: 2120 1148 767 677 408 401 245 73 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5287 NdeI CA'TA_TG Cuts at: 387 387 Size: 5839 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5839 NotI GC'GGCC_GC Cuts at: 3286 3286 Size: 5839 NspI r_CATG'y Cuts at: 1132 1229 3197 4742 1132 Size: 97 1968 1545 2229 Fragments arranged by size: 2229 1968 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5839 PciI A'CATG_T Cuts at: 4738 4738 Size: 5839 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5839 PmeI GTTT'AAAC Cuts at: 3143 3143 Size: 5839 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3908 3940 5421 5453 3908 Size: 32 1481 32 4294 Fragments arranged by size: 4294 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5839 PsiI TTA'TAA Cuts at: 3409 3409 Size: 5839 PstI C_TGCA'G Cuts at: 830 2229 2599 2623 2871 3191 830 Size: 1399 370 24 248 320 3478 Fragments arranged by size: 3478 1399 370 320 248 24 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5839 PvuII CAG'CTG Cuts at: 2014 2854 2014 Size: 840 4999 SacI G_AGCT'C Cuts at: 2257 2257 Size: 5839 SalI G'TCGA_C Cuts at: 1936 2160 3181 1936 Size: 224 1021 4594 Fragments arranged by size: 4594 1021 224 SapI GCTCTTCn'nnn_ Cuts at: 2072 2072 Size: 5839 SbfI CC_TGCA'GG Cuts at: 2229 3191 2229 Size: 962 4877 ScaI AGT'ACT Cuts at: 3088 4087 5799 3088 Size: 999 1712 3128 Fragments arranged by size: 3128 1712 999 SexAI A'CCwGG_T Cuts at: 2296 2296 Size: 5839 SfcI C'TryA_G Cuts at: 826 1045 2225 2595 2619 2867 3187 4322 5003 Size: 219 1180 370 24 248 320 1135 681 Cuts at: 5003 5194 826 Size: 191 1471 Fragments arranged by size: 1471 1180 1135 681 370 320 248 219 191 24 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5839 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5839 SmaI CCC'GGG Cuts at: 1872 3166 1872 Size: 1294 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3943 4844 5106 820 Size: 197 926 2000 901 262 1553 Fragments arranged by size: 2000 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3630 493 Size: 3137 2702 SpeI A'CTAG_T Cuts at: 152 2047 152 Size: 1895 3944 SphI G_CATG'C Cuts at: 1132 3197 1132 Size: 2065 3774 SspI AAT'ATT Cuts at: 5 52 3763 5 Size: 47 3711 2081 Fragments arranged by size: 3711 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5839 StyI C'CwwG_G Cuts at: 513 1065 3278 513 Size: 552 2213 3074 Fragments arranged by size: 3074 2213 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3866 3883 4036 4221 4666 325 Size: 1255 2286 17 153 185 445 1498 Fragments arranged by size: 2286 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3086 4085 5797 Size: 274 80 33 51 1443 1109 999 1712 Cuts at: 5797 96 Size: 138 Fragments arranged by size: 1712 1443 1109 999 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5799 5831 5799 Size: 32 5807 Tth111I GACn'n_nGTC Cuts at: 1316 2298 1316 Size: 982 4857 XbaI T'CTAG_A Cuts at: 3175 3175 Size: 5839 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5839 XmaI C'CCGG_G Cuts at: 1870 3164 1870 Size: 1294 4545 XmnI GAAnn'nnTTC Cuts at: 3968 3968 Size: 5839 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3647 276 Size: 53 83 186 936 2113 2468 Fragments arranged by size: 2468 2113 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI