################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## Acc65I AccI AccII AclI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BpmI BsaAI BsaI BsiEI BsiWI BsmFI BsmI BspDI BspLU11I BsrBI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CfoI ClaI CpoI DpnII DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI HinP1I KasI KpnI Ksp632I KspI MaeIII MboI MflI MluI MluNI MscI MspA1I MvnI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacII SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tsp45I TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AatII 397 GACGTC GACGT^C AccIII 248 TCCGGA T^CCGGA AcyI 397 GACGTC G[GA]^CG[CT]C AfaI 72 GTAC GT^AC AlwNI 593 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG AvaI 227 CTCGGG C^[CT]CG[GA]G BanI 212 GGCACC G^G[CT][GA]CC BanII 292 GAGCTC G[GA]GC[CT]^C BbsI 439 GAAGAC GAAGAC(2/6) BcnI 158 CCGGG CC^[GC]GG BfaI 388 CTAG C^TAG BlpI 186 GCTGAGC GC^T[ACGT]AGC Bpu1102I 186 GCTGAGC GC^T[ACGT]AGC BpuAI 439 GAAGAC GAAGAC(2/6) BsaBI 334 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 397 GACGTC G[GA]^CG[CT]C BseAI 248 TCCGGA T^CCGGA BseRI 418 GAGGAG GAGGAG(10/8) BsgI 514 GTGCAG GTGCAG(16/14) BsiYI 152 CCACCTCCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 152 CCACCTCCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 395 GAGAC GTCTC(1/5) BsmBI 395 GAGACG CGTCTC(1/5) BsoBI 227 CTCGGG C^[CT]CG[GA]G Bsp1407I 71 TGTACA T^GTACA BspEI 248 TCCGGA T^CCGGA BspHI 330 TCATGA T^CATGA BspMI 619 GCAGGT ACCTGC(4/8) BsrDI 614 GCAATG GCAATG(2/0) BsrFI 256 ACCGGC [GA]^CCGG[CT] BsrGI 71 TGTACA T^GTACA BsrI 527 ACTGG ACTGG(1/-1) BssSI 45 CTCGTG CACGAG(-5/-1) BstF5I 75 CATCC GGATG(2/0) BstXI 368 CCACTCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 186 GCTGAGC GC^T[ACGT]AGC Cfr10I 256 ACCGGC [GA]^CCGG[CT] DraII 242 AGGCCCT [GA]G^G[ACGT]CC[CT] EaeI 269 TGGCCG [CT]^GGCC[GA] EcoO109I 242 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoRI 232 GAATTC G^AATTC FauI 275 CCCGC CCCGC(4/6) FokI 75 CATCC GGATG(9/13) Hin1I 397 GACGTC G[GA]^CG[CT]C HindIII 382 AAGCTT A^AGCTT HpaI 588 GTTAAC GTT^AAC MaeI 388 CTAG C^TAG MaeII 398 ACGT A^CGT MamI 334 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 434 CAATTG C^AATTG MroI 248 TCCGGA T^CCGGA MseI 589 TTAA T^TAA MunI 434 CAATTG C^AATTG NciI 158 CCGGG CC^[GC]GG NspI 164 ACATGC [GA]CATG^[CT] RcaI 330 TCATGA T^CATGA RsaI 72 GTAC GT^AC SacI 292 GAGCTC GAGCT^C SspBI 71 TGTACA T^GTACA TaqI 4 TCGA T^CGA Tru9I 589 TTAA T^TAA TthHB8I 4 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AspHI 189 GAGCAC G[AT]GC[AT]^C 292 GAGCTC BmyI 189 GAGCAC G[AGT]GC[ACT]^C 292 GAGCTC BsaWI 248 TCCGGA [AT]^CCGG[AT] 449 ACCGGA BsiHKAI 189 GAGCAC G[AT]GC[AT]^C 292 GAGCTC Bsp1286I 189 GAGCAC G[AGT]GC[ACT]^C 292 GAGCTC BstNI 59 CCAGG CC^[AT]GG 78 CCAGG Cfr13I 56 GGGCC G^G[ACGT]CC 243 GGCCC EcoRII 59 CCAGG ^CC[AT]GG 78 CCAGG EcoT14I 194 CCTTGG C^C[AT][AT]GG 216 CCTTGG HincII 429 GTTGAC GT[CT]^[GA]AC 588 GTTAAC HindII 429 GTTGAC GT[CT]^[GA]AC 588 GTTAAC HphI 63 GGTGA GGTGA(8/7) 123 GGTGA MslI 75 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 369 CACTCTGGTG MvaI 59 CCAGG CC^[AT]GG 78 CCAGG MwoI 596 GCTCTGATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 605 GCTCAGAAAGC NlaIV 84 GGAGCC GG[ACGT]^[ACGT]CC 212 GGCACC PleI 225 GACTC GAGTC(4/5) 206 GAGTC PstI 116 CTGCAG CTGCA^G 461 CTGCAG Sau96I 56 GGGCC G^G[ACGT]CC 243 GGCCC SfcI 116 CTGCAG C^T[GA][CT]AG 461 CTGCAG StyI 194 CCTTGG C^C[AT][AT]GG 216 CCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AciI AcsI AluI ApoI BbvI BsaJI BssKI Cac8I DdeI Fnu4HI HaeIII HapII HinfI HpaII ItaI MboII MnlI MspI NlaIII ScrFI TfiI TseI Tsp509I