(Circular) (Six-base) MAPSORT of: pFN21AE5294.seq Check: 2536 from: 1 to: 5368 pFN21AE5294 5368 bp With 182 enzymes: SgfI * January 30, 2014 17:16 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2406 3178 278 Size: 53 83 186 936 870 772 2468 Fragments arranged by size: 2468 936 870 772 186 83 53 AccI GT'mk_AC Cuts at: 1937 2711 1937 Size: 774 4594 Acc65I G'GTAC_C Cuts at: 2689 2689 Size: 5368 AclI AA'CG_TT Cuts at: 1563 3495 3868 1563 Size: 1932 373 3063 Fragments arranged by size: 3063 1932 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2041 2351 3431 4815 5083 1632 Size: 409 310 1080 1384 268 1917 Fragments arranged by size: 1917 1384 1080 409 310 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5171 AflIII A'CryG_T Cuts at: 1203 4267 5244 1203 Size: 3064 977 1327 Fragments arranged by size: 3064 1327 977 AgeI A'CCGG_T Cuts at: 4199 4199 Size: 5368 AhdI GACnn_n'nnGTC Cuts at: 4097 4097 Size: 5368 AleI CACnn'nnGTG Cuts at: 1166 2378 1166 Size: 1212 4156 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5368 AlwNI CAG_nnn'CTG Cuts at: 2603 3111 4683 2603 Size: 508 1572 3288 Fragments arranged by size: 3288 1572 508 ApaLI G'TGCA_C Cuts at: 3425 4581 3425 Size: 1156 4212 ApoI r'AATT_y Cuts at: 1493 2032 2237 2659 2677 2903 3055 5164 1493 Size: 539 205 422 18 226 152 2109 1697 Fragments arranged by size: 2109 1697 539 422 226 205 152 18 AseI AT'TA_AT Cuts at: 160 1032 3922 160 Size: 872 2890 1606 Fragments arranged by size: 2890 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5368 AvaI C'yCGr_G Cuts at: 1870 1943 2232 2693 1870 Size: 73 289 461 4545 Fragments arranged by size: 4545 461 289 73 BamHI G'GATC_C Cuts at: 2698 3065 2698 Size: 367 5001 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2217 2689 4144 618 Size: 325 217 627 430 472 1455 1842 Fragments arranged by size: 1842 1455 627 472 430 325 217 BanII G_rGCy'C Cuts at: 1391 2301 2687 1391 Size: 910 386 4072 Fragments arranged by size: 4072 910 386 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2451 928 Size: 971 552 3845 Fragments arranged by size: 3845 971 552 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3559 3593 1903 Size: 34 1622 34 3678 Fragments arranged by size: 3678 1622 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3260 4470 3260 Size: 1210 4158 BclI T'GATC_A Cuts at: 1548 1548 Size: 5368 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3979 136 Size: 107 122 71 631 605 2307 1525 Fragments arranged by size: 2307 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5363 5363 Size: 5368 BlpI GC'TnA_GC Cuts at: 2192 2785 2192 Size: 593 4775 Bme1580I G_kGCm'C Cuts at: 1790 3429 4585 1790 Size: 1639 1156 2573 Fragments arranged by size: 2573 1639 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5368 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3099 4057 442 Size: 416 53 83 399 24 1682 958 1753 Fragments arranged by size: 1753 1682 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5368 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4028 1239 Size: 2789 2579 Bpu10I CC'TnA_GC Cuts at: 2295 2295 Size: 5368 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3493 4358 4656 3493 Size: 865 298 4205 Fragments arranged by size: 4205 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4031 882 Size: 617 2532 2219 Fragments arranged by size: 2532 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3159 493 Size: 711 1955 2702 Fragments arranged by size: 2702 1955 711 BsaBI GATnn'nnATC Cuts at: 2343 5362 2343 Size: 3019 2349 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2403 3175 3557 4192 Size: 53 83 186 936 870 772 382 635 Cuts at: 4192 275 Size: 1451 Fragments arranged by size: 1451 936 870 772 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2253 2454 3801 4199 4473 4620 5031 2253 Size: 201 1347 398 274 147 411 2590 Fragments arranged by size: 2590 1347 411 398 274 201 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2417 2447 1178 Size: 30 165 30 1014 30 4099 Fragments arranged by size: 4099 1014 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2438 1186 Size: 1252 4116 BseYI C'CCAG_C Cuts at: 4571 5067 4571 Size: 496 4872 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2540 1163 Size: 573 804 3991 Fragments arranged by size: 3991 804 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2818 3579 4607 1540 Size: 275 186 817 761 1028 2301 Fragments arranged by size: 2301 1028 817 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2198 2301 3429 3514 4585 2198 Size: 103 1128 85 1071 2981 Fragments arranged by size: 2981 1128 1071 103 85 BsmI GAATG_Cn' Cuts at: 2879 2972 2879 Size: 93 5275 BsmBI CGTCTCn'nnnn_ Cuts at: 2394 2394 Size: 5368 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2198 2301 2687 3429 3514 4585 1391 Size: 399 408 103 386 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 408 399 386 103 85 BspEI T'CCGG_A Cuts at: 2253 5031 2253 Size: 2778 2590 BspHI T'CATG_A Cuts at: 2335 3257 4987 2335 Size: 922 1730 2716 Fragments arranged by size: 2716 1730 922 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2615 2723 844 Size: 1771 108 3489 Fragments arranged by size: 3489 1771 108 BsrBI CCG'CTC Cuts at: 1394 1823 3255 1394 Size: 429 1432 3507 Fragments arranged by size: 3507 1432 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2626 3863 4037 65 Size: 1183 1378 1237 174 1396 Fragments arranged by size: 1396 1378 1237 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2261 4012 4199 1700 Size: 110 451 1751 187 2869 Fragments arranged by size: 2869 1751 451 187 110 BsrGI T'GTAC_A Cuts at: 96 2076 96 Size: 1980 3388 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5368 BssSI C'ACGA_G Cuts at: 2050 3428 4440 2050 Size: 1378 1012 2978 Fragments arranged by size: 2978 1378 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5368 BstBI TT'CG_AA Cuts at: 4172 4172 Size: 5368 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5368 BstXI CCAn_nnnn'nTGG Cuts at: 2380 2380 Size: 5368 BstYI r'GATC_y Cuts at: 1972 2698 3065 3450 3467 4919 5005 5017 5363 Size: 726 367 385 17 1452 86 12 346 Cuts at: 5363 1972 Size: 1977 Fragments arranged by size: 1977 1452 726 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4816 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4629 Fragments arranged by size: 4629 683 56 BtsI GCAGTG_nn' Cuts at: 2884 3678 3698 2884 Size: 794 20 4554 Fragments arranged by size: 4554 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5368 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5333 DraI TTT'AAA Cuts at: 2672 2672 Size: 5368 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4375 809 Size: 507 3059 1802 Fragments arranged by size: 3059 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2274 2815 3704 Size: 54 1425 50 275 18 444 541 889 Cuts at: 3704 5333 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 541 444 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2815 1537 Size: 275 1003 4090 Fragments arranged by size: 4090 1003 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3298 1353 Size: 1945 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3955 4329 4475 387 Size: 1064 138 2366 374 146 1280 Fragments arranged by size: 2366 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2299 2299 Size: 5368 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2041 2351 3431 4028 4815 5083 1239 Size: 393 409 310 1080 597 787 268 1524 Fragments arranged by size: 1524 1080 787 597 409 393 310 268 EcoO109I rG'GnC_Cy Cuts at: 2248 2248 Size: 5368 EcoRI G'AATT_C Cuts at: 2237 2677 3055 2237 Size: 440 378 4550 Fragments arranged by size: 4550 440 378 FspI TGC'GCA Cuts at: 3874 3874 Size: 5368 HaeII r_GCGC'y Cuts at: 1678 4515 1678 Size: 2837 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2297 2329 4009 4041 4083 Size: 32 135 32 725 32 1680 32 42 Cuts at: 4083 4115 1373 Size: 32 2626 Fragments arranged by size: 2626 1680 725 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2436 2595 2712 2958 669 Size: 988 281 498 159 117 246 3079 Fragments arranged by size: 3079 988 498 281 246 159 117 HindIII A'AGCT_T Cuts at: 748 2387 748 Size: 1639 3729 HpaI GTT'AAC Cuts at: 2595 2958 2595 Size: 363 5005 KpnI G_GTAC'C Cuts at: 2693 2693 Size: 5368 MfeI C'AATT_G Cuts at: 2439 2967 2439 Size: 528 4840 MluI A'CGCG_T Cuts at: 5244 5244 Size: 5368 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2207 2374 4482 4666 1305 Size: 902 167 2108 184 2007 Fragments arranged by size: 2108 2007 902 184 167 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3546 Fragments arranged by size: 3546 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2084 2378 3326 3685 3844 518 Size: 648 918 294 948 359 159 2042 Fragments arranged by size: 2042 948 918 648 359 294 159 MspA1I CmG'CkG Cuts at: 1606 2784 3461 4609 4854 1606 Size: 1178 677 1148 245 2120 Fragments arranged by size: 2120 1178 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4816 NdeI CA'TA_TG Cuts at: 387 387 Size: 5368 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5368 NotI GC'GGCC_GC Cuts at: 2815 2815 Size: 5368 NspI r_CATG'y Cuts at: 1132 1229 2173 2726 4271 1132 Size: 97 944 553 1545 2229 Fragments arranged by size: 2229 1545 944 553 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5368 PciI A'CATG_T Cuts at: 4267 4267 Size: 5368 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5368 PfoI T'CCnGG_A Cuts at: 2162 2162 Size: 5368 PmeI GTTT'AAAC Cuts at: 2672 2672 Size: 5368 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3437 3469 4950 4982 3437 Size: 32 1481 32 3823 Fragments arranged by size: 3823 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5368 PsiI TTA'TAA Cuts at: 2938 2938 Size: 5368 PstI C_TGCA'G Cuts at: 830 2125 2470 2720 830 Size: 1295 345 250 3478 Fragments arranged by size: 3478 1295 345 250 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5368 SacI G_AGCT'C Cuts at: 2301 2301 Size: 5368 SalI G'TCGA_C Cuts at: 1936 2710 1936 Size: 774 4594 SbfI CC_TGCA'GG Cuts at: 2720 2720 Size: 5368 ScaI AGT'ACT Cuts at: 3616 5328 3616 Size: 1712 3656 SfcI C'TryA_G Cuts at: 826 1045 2121 2466 2716 3851 4532 4723 826 Size: 219 1076 345 250 1135 681 191 1471 Fragments arranged by size: 1471 1135 1076 681 345 250 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5368 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5368 SmaI CCC'GGG Cuts at: 1872 2695 1872 Size: 823 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3472 4373 4635 820 Size: 197 926 1529 901 262 1553 Fragments arranged by size: 1553 1529 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3159 493 Size: 2666 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5368 SphI G_CATG'C Cuts at: 1132 2726 1132 Size: 1594 3774 SspI AAT'ATT Cuts at: 5 52 3292 5 Size: 47 3240 2081 Fragments arranged by size: 3240 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5368 StyI C'CwwG_G Cuts at: 513 1065 2199 2221 2807 513 Size: 552 1134 22 586 3074 Fragments arranged by size: 3074 1134 586 552 22 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3395 3412 3565 3750 4195 325 Size: 1255 1815 17 153 185 445 1498 Fragments arranged by size: 1815 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2076 3614 5326 Size: 274 80 33 51 1443 99 1538 1712 Cuts at: 5326 96 Size: 138 Fragments arranged by size: 1712 1538 1443 274 138 99 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5328 5360 5328 Size: 32 5336 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5368 XbaI T'CTAG_A Cuts at: 2704 2704 Size: 5368 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5368 XmaI C'CCGG_G Cuts at: 1870 2693 1870 Size: 823 4545 XmnI GAAnn'nnTTC Cuts at: 3497 3497 Size: 5368 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2404 3176 276 Size: 53 83 186 936 870 772 2468 Fragments arranged by size: 2468 936 870 772 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI