(Linear) (Six-base) MAPSORT of: KIEE5294.seq Check: 230 from: 1 to: 663 KIEE5294.orf With 182 enzymes: SgfI * January 29, 2014 11:54 .. AatII G_ACGT'C Cuts at: 0 401 663 Size: 401 262 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 36 346 663 Size: 36 310 317 Fragments arranged by size: 317 310 36 AleI CACnn'nnGTG Cuts at: 0 373 663 Size: 373 290 AlwNI CAG_nnn'CTG Cuts at: 0 598 663 Size: 598 65 ApoI r'AATT_y Cuts at: 0 27 232 654 663 Size: 27 205 422 9 Fragments arranged by size: 422 205 27 9 AvaI C'yCGr_G Cuts at: 0 227 663 Size: 227 436 BanI G'GyrC_C Cuts at: 0 212 663 Size: 212 451 BanII G_rGCy'C Cuts at: 0 296 663 Size: 296 367 BbsI GAAGACnn'nnnn_ Cuts at: 0 446 663 Size: 446 217 BlpI GC'TnA_GC Cuts at: 0 187 663 Size: 187 476 Bpu10I CC'TnA_GC Cuts at: 0 290 663 Size: 290 373 BsaBI GATnn'nnATC Cuts at: 0 338 663 Size: 338 325 BsaHI Gr'CG_yC Cuts at: 0 398 663 Size: 398 265 BsaWI w'CCGG_w Cuts at: 0 248 449 663 Size: 248 201 214 Fragments arranged by size: 248 214 201 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 412 442 663 Size: 412 30 221 Fragments arranged by size: 412 221 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 433 663 Size: 433 230 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 535 663 Size: 535 128 BsiHKAI G_wGCw'C Cuts at: 0 193 296 663 Size: 193 103 367 Fragments arranged by size: 367 193 103 BsmBI CGTCTCn'nnnn_ Cuts at: 0 389 663 Size: 389 274 Bsp1286I G_dGCh'C Cuts at: 0 193 296 663 Size: 193 103 367 Fragments arranged by size: 367 193 103 BspEI T'CCGG_A Cuts at: 0 248 663 Size: 248 415 BspHI T'CATG_A Cuts at: 0 330 663 Size: 330 333 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 610 663 Size: 610 53 BsrDI GCAATG_nn' Cuts at: 0 621 663 Size: 621 42 BsrFI r'CCGG_y Cuts at: 0 256 663 Size: 256 407 BsrGI T'GTAC_A Cuts at: 0 71 663 Size: 71 592 BssSI C'ACGA_G Cuts at: 0 45 663 Size: 45 618 BstXI CCAn_nnnn'nTGG Cuts at: 0 375 663 Size: 375 288 EaeI y'GGCC_r Cuts at: 0 269 663 Size: 269 394 EcoICRI GAG'CTC Cuts at: 0 294 663 Size: 294 369 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 36 346 663 Size: 36 310 317 Fragments arranged by size: 317 310 36 EcoO109I rG'GnC_Cy Cuts at: 0 243 663 Size: 243 420 EcoRI G'AATT_C Cuts at: 0 232 663 Size: 232 431 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 292 324 663 Size: 292 32 339 Fragments arranged by size: 339 292 32 HincII GTy'rAC Cuts at: 0 431 590 663 Size: 431 159 73 Fragments arranged by size: 431 159 73 HindIII A'AGCT_T Cuts at: 0 382 663 Size: 382 281 HpaI GTT'AAC Cuts at: 0 590 663 Size: 590 73 MfeI C'AATT_G Cuts at: 0 434 663 Size: 434 229 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 202 369 663 Size: 202 167 294 Fragments arranged by size: 294 202 167 MslI CAynn'nnrTG Cuts at: 0 79 373 663 Size: 79 294 290 Fragments arranged by size: 294 290 79 NspI r_CATG'y Cuts at: 0 168 663 Size: 168 495 PfoI T'CCnGG_A Cuts at: 0 157 663 Size: 157 506 PstI C_TGCA'G Cuts at: 0 120 465 663 Size: 120 345 198 Fragments arranged by size: 345 198 120 SacI G_AGCT'C Cuts at: 0 296 663 Size: 296 367 SfcI C'TryA_G Cuts at: 0 116 461 663 Size: 116 345 202 Fragments arranged by size: 345 202 116 StyI C'CwwG_G Cuts at: 0 194 216 663 Size: 194 22 447 Fragments arranged by size: 447 194 22 TatI w'GTAC_w Cuts at: 0 71 663 Size: 71 592 ZraI GAC'GTC Cuts at: 0 399 663 Size: 399 264 Enzymes that do cut: AatII AcuI AleI AlwNI ApoI AvaI BanI BanII BbsI BlpI Bpu10I BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI BssSI BstXI EaeI EcoICRI Eco57MI EcoO109I EcoRI Hin4I HincII HindIII HpaI MfeI MmeI MslI NspI PfoI PstI SacI SfcI StyI TatI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BseYI BsiEI BsiWI BsmI BsrBI BssHII BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EarI EciI EcoNI EcoRV FalI FseI FspI FspAI HaeII KasI KpnI MluI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI XmnI