(Circular) (Six-base) MAPSORT of: pFN21AE6293.seq Check: 8568 from: 1 to: 5437 pFN21AE6293 5437 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2411 2411 Size: 5437 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3247 278 Size: 53 83 186 936 1711 2468 Fragments arranged by size: 2468 1711 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2780 1937 Size: 843 4594 Acc65I G'GTAC_C Cuts at: 2758 2758 Size: 5437 AclI AA'CG_TT Cuts at: 1563 3564 3937 1563 Size: 2001 373 3063 Fragments arranged by size: 3063 2001 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3500 4884 5152 1632 Size: 1868 1384 268 1917 Fragments arranged by size: 1917 1868 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5240 AflIII A'CryG_T Cuts at: 1203 4336 5313 1203 Size: 3133 977 1327 Fragments arranged by size: 3133 1327 977 AgeI A'CCGG_T Cuts at: 2177 4268 2177 Size: 2091 3346 AhdI GACnn_n'nnGTC Cuts at: 4166 4166 Size: 5437 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5437 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5437 AlwNI CAG_nnn'CTG Cuts at: 3180 4752 3180 Size: 1572 3865 ApaI G_GGCC'C Cuts at: 2717 2717 Size: 5437 ApaLI G'TGCA_C Cuts at: 3494 4650 3494 Size: 1156 4281 ApoI r'AATT_y Cuts at: 1493 2183 2288 2746 2972 3124 5233 1493 Size: 690 105 458 226 152 2109 1697 Fragments arranged by size: 2109 1697 690 458 226 152 105 AseI AT'TA_AT Cuts at: 160 1032 3991 160 Size: 872 2959 1606 Fragments arranged by size: 2959 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5437 AvaI C'yCGr_G Cuts at: 1870 1943 2489 2592 2726 2762 1870 Size: 73 546 103 134 36 4545 Fragments arranged by size: 4545 546 134 103 73 36 BamHI G'GATC_C Cuts at: 2258 2767 3134 2258 Size: 509 367 4561 Fragments arranged by size: 4561 509 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2497 2758 4213 618 Size: 325 217 627 710 261 1455 1842 Fragments arranged by size: 1842 1455 710 627 325 261 217 BanII G_rGCy'C Cuts at: 1391 2488 2717 2756 1391 Size: 1097 229 39 4072 Fragments arranged by size: 4072 1097 229 39 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2199 2540 928 Size: 971 300 341 3825 Fragments arranged by size: 3825 971 341 300 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2347 2381 3628 3662 1903 Size: 34 410 34 1247 34 3678 Fragments arranged by size: 3678 1247 410 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3329 4539 3329 Size: 1210 4227 BclI T'GATC_A Cuts at: 1548 1548 Size: 5437 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4048 136 Size: 107 122 71 631 605 2376 1525 Fragments arranged by size: 2376 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2538 5432 2538 Size: 2894 2543 BlpI GC'TnA_GC Cuts at: 2854 2854 Size: 5437 Bme1580I G_kGCm'C Cuts at: 1790 2502 2717 3498 4654 1790 Size: 712 215 781 1156 2573 Fragments arranged by size: 2573 1156 781 712 215 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5437 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3168 4126 442 Size: 416 53 83 399 24 1751 958 1753 Fragments arranged by size: 1753 1751 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5437 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4097 1239 Size: 2858 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3562 4427 4725 3562 Size: 865 298 4274 Fragments arranged by size: 4274 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2688 4100 882 Size: 617 1189 1412 2219 Fragments arranged by size: 2219 1412 1189 617 BsaAI yAC'GTr Cuts at: 493 1204 3228 493 Size: 711 2024 2702 Fragments arranged by size: 2702 2024 711 BsaBI GATnn'nnATC Cuts at: 5431 5431 Size: 5437 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3244 3626 4261 275 Size: 53 83 186 936 1711 382 635 1451 Fragments arranged by size: 1711 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2177 3870 4268 4542 4689 5100 2177 Size: 1693 398 274 147 411 2514 Fragments arranged by size: 2514 1693 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5212 Fragments arranged by size: 5212 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5437 BseYI C'CCAG_C Cuts at: 2128 2502 4640 5136 2128 Size: 374 2138 496 2429 Fragments arranged by size: 2429 2138 496 374 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2439 1163 Size: 573 703 4161 Fragments arranged by size: 4161 703 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2887 3648 4676 1540 Size: 275 186 886 761 1028 2301 Fragments arranged by size: 2301 1028 886 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2601 3498 3583 4654 2601 Size: 897 85 1071 3384 Fragments arranged by size: 3384 1071 897 85 BsmI GAATG_Cn' Cuts at: 2948 3041 2948 Size: 93 5344 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2488 2502 2601 2717 2756 3498 3583 Size: 399 698 14 99 116 39 742 85 Cuts at: 3583 4654 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 698 399 116 99 85 39 14 BspEI T'CCGG_A Cuts at: 5100 5100 Size: 5437 BspHI T'CATG_A Cuts at: 3326 5056 3326 Size: 1730 3707 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2411 2484 2792 844 Size: 1567 73 308 3489 Fragments arranged by size: 3489 1567 308 73 BsrBI CCG'CTC Cuts at: 1394 1823 3324 1394 Size: 429 1501 3507 Fragments arranged by size: 3507 1501 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3932 4106 65 Size: 1183 2684 174 1396 Fragments arranged by size: 2684 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2177 4081 4268 1700 Size: 110 367 1904 187 2869 Fragments arranged by size: 2869 1904 367 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5437 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5437 BssSI C'ACGA_G Cuts at: 3497 4509 3497 Size: 1012 4425 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5437 BstBI TT'CG_AA Cuts at: 4241 4241 Size: 5437 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5437 BstXI CCAn_nnnn'nTGG Cuts at: 2404 2543 2404 Size: 139 5298 BstYI r'GATC_y Cuts at: 1972 2258 2538 2767 3134 3519 3536 4988 5074 Size: 286 280 229 367 385 17 1452 86 Cuts at: 5074 5086 5432 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 286 280 229 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3946 Fragments arranged by size: 3946 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4698 Fragments arranged by size: 4698 683 56 BtsI GCAGTG_nn' Cuts at: 2132 2953 3747 3767 2132 Size: 821 794 20 3802 Fragments arranged by size: 3802 821 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5437 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2359 2394 2605 2640 529 Size: 35 1795 35 211 35 3326 Fragments arranged by size: 3326 1795 211 35 35 35 DraI TTT'AAA Cuts at: 2741 2741 Size: 5437 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4444 809 Size: 507 3128 1802 Fragments arranged by size: 3128 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2884 3773 5402 Size: 54 1425 50 275 18 1054 889 1629 Cuts at: 5402 8 Size: 43 Fragments arranged by size: 1629 1425 1054 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2884 1537 Size: 275 1072 4090 Fragments arranged by size: 4090 1072 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2513 2691 3367 1353 Size: 1160 178 676 3423 Fragments arranged by size: 3423 1160 676 178 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4024 4398 4544 387 Size: 1064 138 2435 374 146 1280 Fragments arranged by size: 2435 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3500 4097 4884 5152 1239 Size: 393 1868 597 787 268 1524 Fragments arranged by size: 1868 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2304 2713 2304 Size: 409 5028 EcoRI G'AATT_C Cuts at: 2183 2746 3124 2183 Size: 563 378 4496 Fragments arranged by size: 4496 563 378 FspI TGC'GCA Cuts at: 3943 3943 Size: 5437 HaeII r_GCGC'y Cuts at: 1678 4584 1678 Size: 2906 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2238 2270 2530 2562 4078 Size: 32 135 32 666 32 260 32 1516 Cuts at: 4078 4110 4152 4184 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1516 666 260 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2122 2781 3027 669 Size: 988 281 184 659 246 3079 Fragments arranged by size: 3079 988 659 281 246 184 HindIII A'AGCT_T Cuts at: 748 748 Size: 5437 HpaI GTT'AAC Cuts at: 3027 3027 Size: 5437 KpnI G_GTAC'C Cuts at: 2762 2762 Size: 5437 MfeI C'AATT_G Cuts at: 3036 3036 Size: 5437 MluI A'CGCG_T Cuts at: 5313 5313 Size: 5437 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2457 4551 4735 1305 Size: 1152 2094 184 2007 Fragments arranged by size: 2094 2007 1152 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3615 Fragments arranged by size: 3615 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2528 3395 3754 3913 518 Size: 648 1362 867 359 159 2042 Fragments arranged by size: 2042 1362 867 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2853 3530 4678 4923 1606 Size: 1247 677 1148 245 2120 Fragments arranged by size: 2120 1247 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3946 Fragments arranged by size: 3946 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5437 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5437 NotI GC'GGCC_GC Cuts at: 2884 2884 Size: 5437 NspI r_CATG'y Cuts at: 1132 1229 2795 4340 1132 Size: 97 1566 1545 2229 Fragments arranged by size: 2229 1566 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5437 PciI A'CATG_T Cuts at: 4336 4336 Size: 5437 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5437 PmeI GTTT'AAAC Cuts at: 2741 2741 Size: 5437 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3506 3538 5019 5051 3506 Size: 32 1481 32 3892 Fragments arranged by size: 3892 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5437 PsiI TTA'TAA Cuts at: 3007 3007 Size: 5437 PspOMI G'GGCC_C Cuts at: 2713 2713 Size: 5437 PstI C_TGCA'G Cuts at: 830 2677 2789 830 Size: 1847 112 3478 Fragments arranged by size: 3478 1847 112 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5437 SalI G'TCGA_C Cuts at: 1936 2779 1936 Size: 843 4594 SapI GCTCTTCn'nnn_ Cuts at: 2513 2513 Size: 5437 SbfI CC_TGCA'GG Cuts at: 2789 2789 Size: 5437 ScaI AGT'ACT Cuts at: 3685 5397 3685 Size: 1712 3725 SfcI C'TryA_G Cuts at: 826 1045 2673 2785 3920 4601 4792 826 Size: 219 1628 112 1135 681 191 1471 Fragments arranged by size: 1628 1471 1135 681 219 191 112 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5437 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5437 SmaI CCC'GGG Cuts at: 1872 2764 1872 Size: 892 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3541 4442 4704 820 Size: 197 926 1598 901 262 1553 Fragments arranged by size: 1598 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3228 493 Size: 2735 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5437 SphI G_CATG'C Cuts at: 1132 2795 1132 Size: 1663 3774 SspI AAT'ATT Cuts at: 5 52 3361 5 Size: 47 3309 2081 Fragments arranged by size: 3309 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5437 StyI C'CwwG_G Cuts at: 513 1065 2004 2277 2623 2706 2876 513 Size: 552 939 273 346 83 170 3074 Fragments arranged by size: 3074 939 552 346 273 170 83 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3464 3481 3634 3819 4264 325 Size: 1255 1884 17 153 185 445 1498 Fragments arranged by size: 1884 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2448 3683 5395 Size: 274 80 33 51 1443 471 1235 1712 Cuts at: 5395 96 Size: 138 Fragments arranged by size: 1712 1443 1235 471 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5397 5429 5397 Size: 32 5405 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5437 XbaI T'CTAG_A Cuts at: 2773 2773 Size: 5437 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2404 2404 Size: 5437 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5437 XmaI C'CCGG_G Cuts at: 1870 2762 1870 Size: 892 4545 XmnI GAAnn'nnTTC Cuts at: 3566 3566 Size: 5437 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3245 276 Size: 53 83 186 936 1711 2468 Fragments arranged by size: 2468 1711 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI