################################## # NO CUT ENZYMES ( 169 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BbeI BbsI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DpnII DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I MaeI MamI MboI MboII MfeI MflI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmeI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SapI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AatII 415 GACGTC GACGT^C AfaI 135 GTAC GT^AC ApaI 303 GGGCCC GGGCC^C BanII 303 GGGCCC G[GA]GC[CT]^C BbrPI 139 CACGTG CAC^GTG BbvCI 198 GCTGAGG CCTCAGC(-5/-2) BsaAI 139 CACGTG [CT]AC^GT[GA] BsgI 292 GTGCAG GTGCAG(16/14) BsmAI 418 GTCTC GTCTC(1/5) BsmBI 417 CGTCTC CGTCTC(1/5) BsmFI 95 GGGAC GGGAC(10/14) BsrBI 147 GAGCGG CCGCTC(-3/-3) BstXI 329 CCAGCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DdeI 199 CTGAG C^T[ACGT]AG EcoNI 333 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 218 CCAAGG C^C[AT][AT]GG HaeII 162 GGCGCT [GA]GCGC^[CT] HphI 370 GGTGA GGTGA(8/7) KspI 504 CCGCGG CCGC^GG MaeIII 297 GTCAC ^GT[ACGT]AC MseI 518 TTAA T^TAA MspA1I 504 CCGCGG C[AC]G^C[GT]G PmaCI 139 CACGTG CAC^GTG PmlI 139 CACGTG CAC^GTG PpuMI 341 AGGACCT [GA]G^G[AT]CC[CT] PstI 315 CTGCAG CTGCA^G RsaI 135 GTAC GT^AC SacII 504 CCGCGG CCGC^GG SfcI 315 CTGCAG C^T[GA][CT]AG SmaI 393 CCCGGG CCC^GGG StyI 218 CCAAGG C^C[AT][AT]GG Tru9I 518 TTAA T^TAA Tsp45I 297 GTCAC ^GT[GC]AC Tsp509I 520 AATT ^AATT TspRI 193 CAGTG CA[GC]TG[ACGT][ACGT]^ XmaCI 393 CCCGGG C^CCGGG XmaI 393 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AcyI 35 GACGCC G[GA]^CG[CT]C 415 GACGTC AvaI 54 CCCGAG C^[CT]CG[GA]G 393 CCCGGG BmyI 303 GGGCCC G[AGT]GC[ACT]^C 389 GTGCCC BpmI 232 CTGGAG CTGGAG(16/14) 247 CTGGAG BsaHI 35 GACGCC G[GA]^CG[CT]C 415 GACGTC BsoBI 54 CCCGAG C^[CT]CG[GA]G 393 CCCGGG Bsp1286I 303 GGGCCC G[AGT]GC[ACT]^C 389 GTGCCC BsrI 132 CCAGT ACTGG(1/-1) 192 CCAGT BssHII 211 GCGCGC G^CGCGC 263 GCGCGC DsaI 7 CCACGG C^C[GA][CT]GG 504 CCGCGG Hin1I 35 GACGCC G[GA]^CG[CT]C 415 GACGTC MaeII 140 ACGT A^CGT 416 ACGT MslI 348 CACACAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 350 CACAGATGTG SfaNI 228 GATGC GCATC(5/9) 279 GATGC ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AccII AciI AluI AlwNI AvaII BbvI BcnI BsaJI BsiYI BslI BssKI BstNI BstUI Cac8I CfoI Cfr13I DraII EcoO109I EcoRII FauI Fnu4HI HaeIII HapII HgaI HhaI HinP1I HpaII ItaI MnlI MspI MvaI MvnI MwoI NciI NlaIV Sau96I ScrFI TseI