(Circular) (Six-base) MAPSORT of: pFN21AE5293.seq Check: 2282 from: 1 to: 5239 pFN21AE5293 5239 bp With 182 enzymes: SgfI * January 30, 2014 17:16 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2424 3049 278 Size: 53 83 186 936 888 625 2468 Fragments arranged by size: 2468 936 888 625 186 83 53 AccI GT'mk_AC Cuts at: 1937 2582 1937 Size: 645 4594 Acc65I G'GTAC_C Cuts at: 2560 2560 Size: 5239 AclI AA'CG_TT Cuts at: 1563 3366 3739 1563 Size: 1803 373 3063 Fragments arranged by size: 3063 1803 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3302 4686 4954 1632 Size: 1670 1384 268 1917 Fragments arranged by size: 1917 1670 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5042 AflIII A'CryG_T Cuts at: 1203 4138 5115 1203 Size: 2935 977 1327 Fragments arranged by size: 2935 1327 977 AgeI A'CCGG_T Cuts at: 4070 4070 Size: 5239 AhdI GACnn_n'nnGTC Cuts at: 3968 3968 Size: 5239 AleI CACnn'nnGTG Cuts at: 1166 2359 1166 Size: 1193 4046 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5239 AlwNI CAG_nnn'CTG Cuts at: 2081 2119 2203 2236 2263 2982 4554 2081 Size: 38 84 33 27 719 1572 2766 Fragments arranged by size: 2766 1572 719 84 38 33 27 ApaI G_GGCC'C Cuts at: 2312 2312 Size: 5239 ApaLI G'TGCA_C Cuts at: 3296 4452 3296 Size: 1156 4083 ApoI r'AATT_y Cuts at: 1493 2548 2774 2926 5035 1493 Size: 1055 226 152 2109 1697 Fragments arranged by size: 2109 1697 1055 226 152 AseI AT'TA_AT Cuts at: 160 1032 3793 160 Size: 872 2761 1606 Fragments arranged by size: 2761 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5239 AvaI C'yCGr_G Cuts at: 1870 1943 2059 2398 2564 1870 Size: 73 116 339 166 4545 Fragments arranged by size: 4545 339 166 116 73 BamHI G'GATC_C Cuts at: 2569 2936 2569 Size: 367 4872 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2560 4015 618 Size: 325 217 627 773 1455 1842 Fragments arranged by size: 1842 1455 773 627 325 217 BanII G_rGCy'C Cuts at: 1391 2312 2558 1391 Size: 921 246 4072 Fragments arranged by size: 4072 921 246 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4268 BbvCI CC'TCA_GC Cuts at: 2204 2204 Size: 5239 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3430 3464 1903 Size: 34 50 34 1409 34 3678 Fragments arranged by size: 3678 1409 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3131 4341 3131 Size: 1210 4029 BclI T'GATC_A Cuts at: 1548 1548 Size: 5239 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3850 136 Size: 107 122 71 631 605 2178 1525 Fragments arranged by size: 2178 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5234 5234 Size: 5239 BlpI GC'TnA_GC Cuts at: 2656 2656 Size: 5239 Bme1580I G_kGCm'C Cuts at: 1790 2312 2398 3300 4456 1790 Size: 522 86 902 1156 2573 Fragments arranged by size: 2573 1156 902 522 86 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5239 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2970 3928 442 Size: 416 53 83 399 24 1553 958 1753 Fragments arranged by size: 1753 1553 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5239 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2236 2236 Size: 5239 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2258 2273 3899 1239 Size: 1019 15 1626 2579 Fragments arranged by size: 2579 1626 1019 15 Bpu10I CC'TnA_GC Cuts at: 2204 2204 Size: 5239 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3364 4229 4527 3364 Size: 865 298 4076 Fragments arranged by size: 4076 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3902 882 Size: 617 2403 2219 Fragments arranged by size: 2403 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2146 3030 493 Size: 711 942 884 2702 Fragments arranged by size: 2702 942 884 711 BsaBI GATnn'nnATC Cuts at: 5233 5233 Size: 5239 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2041 2421 3046 3428 Size: 53 83 186 936 508 380 625 382 Cuts at: 3428 4063 275 Size: 635 1451 Fragments arranged by size: 1451 936 635 625 508 382 380 186 83 53 BsaWI w'CCGG_w Cuts at: 3672 4070 4344 4491 4902 3672 Size: 398 274 147 411 4009 Fragments arranged by size: 4009 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5014 Fragments arranged by size: 5014 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5239 BseYI C'CCAG_C Cuts at: 2112 2170 2276 4442 4938 2112 Size: 58 106 2166 496 2413 Fragments arranged by size: 2413 2166 496 106 58 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2318 1163 Size: 573 582 4084 Fragments arranged by size: 4084 582 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2689 3450 4478 1540 Size: 275 186 688 761 1028 2301 Fragments arranged by size: 2301 1028 761 688 275 186 BsiHKAI G_wGCw'C Cuts at: 3300 3385 4456 3300 Size: 85 1071 4083 Fragments arranged by size: 4083 1071 85 BsmI GAATG_Cn' Cuts at: 2750 2843 2750 Size: 93 5146 BsmBI CGTCTCn'nnnn_ Cuts at: 2428 2428 Size: 5239 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2312 2398 2558 3300 3385 4456 1391 Size: 399 522 86 160 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 522 399 160 86 85 BspEI T'CCGG_A Cuts at: 4902 4902 Size: 5239 BspHI T'CATG_A Cuts at: 3128 4858 3128 Size: 1730 3509 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2594 844 Size: 1750 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2154 3126 1394 Size: 429 331 972 3507 Fragments arranged by size: 3507 972 429 331 BsrDI GCAATG_nn' Cuts at: 65 1248 3734 3908 65 Size: 1183 2486 174 1396 Fragments arranged by size: 2486 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3883 4070 1700 Size: 110 2073 187 2869 Fragments arranged by size: 2869 2073 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5239 BssHII G'CGCG_C Cuts at: 1925 2216 2268 1925 Size: 291 52 4896 Fragments arranged by size: 4896 291 52 BssSI C'ACGA_G Cuts at: 3299 4311 3299 Size: 1012 4227 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5239 BstBI TT'CG_AA Cuts at: 4043 4043 Size: 5239 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5239 BstXI CCAn_nnnn'nTGG Cuts at: 2341 2341 Size: 5239 BstYI r'GATC_y Cuts at: 1972 2569 2936 3321 3338 4790 4876 4888 5234 Size: 597 367 385 17 1452 86 12 346 Cuts at: 5234 1972 Size: 1977 Fragments arranged by size: 1977 1452 597 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2012 2509 513 Size: 552 947 497 3243 Fragments arranged by size: 3243 947 552 497 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2443 487 Size: 683 56 1217 3283 Fragments arranged by size: 3283 1217 683 56 BtsI GCAGTG_nn' Cuts at: 2755 3549 3569 2755 Size: 794 20 4425 Fragments arranged by size: 4425 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5239 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5204 DraI TTT'AAA Cuts at: 2543 2543 Size: 5239 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4246 809 Size: 507 2930 1802 Fragments arranged by size: 2930 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2686 3575 5204 Size: 54 1425 50 275 18 856 889 1629 Cuts at: 5204 8 Size: 43 Fragments arranged by size: 1629 1425 889 856 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2686 1537 Size: 275 874 4090 Fragments arranged by size: 4090 874 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3169 1353 Size: 1816 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3826 4200 4346 387 Size: 1064 138 2237 374 146 1280 Fragments arranged by size: 2237 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2258 2273 3302 3899 4686 4954 1239 Size: 393 626 15 1029 597 787 268 1524 Fragments arranged by size: 1524 1029 787 626 597 393 268 15 EcoNI CCTnn'n_nnAGG Cuts at: 2342 2342 Size: 5239 EcoO109I rG'GnC_Cy Cuts at: 2308 2309 2347 2414 2308 Size: 1 38 67 5133 Fragments arranged by size: 5133 67 38 1 EcoRI G'AATT_C Cuts at: 2548 2926 2548 Size: 378 4861 FspI TGC'GCA Cuts at: 3745 3745 Size: 5239 HaeII r_GCGC'y Cuts at: 1678 2171 4386 1678 Size: 493 2215 2531 Fragments arranged by size: 2531 2215 493 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3880 3912 3954 3986 1373 Size: 32 135 32 2308 32 42 32 2626 Fragments arranged by size: 2626 2308 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2583 2829 669 Size: 988 281 645 246 3079 Fragments arranged by size: 3079 988 645 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5239 HpaI GTT'AAC Cuts at: 2829 2829 Size: 5239 KpnI G_GTAC'C Cuts at: 2564 2564 Size: 5239 MfeI C'AATT_G Cuts at: 2838 2838 Size: 5239 MluI A'CGCG_T Cuts at: 5115 5115 Size: 5239 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4353 4537 1305 Size: 3048 184 2007 Fragments arranged by size: 3048 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3417 Fragments arranged by size: 3417 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2357 2359 3197 3556 3715 518 Size: 648 1191 2 838 359 159 2042 Fragments arranged by size: 2042 1191 838 648 359 159 2 MspA1I CmG'CkG Cuts at: 1606 2511 2655 3332 4480 4725 1606 Size: 905 144 677 1148 245 2120 Fragments arranged by size: 2120 1148 905 677 245 144 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4687 NdeI CA'TA_TG Cuts at: 387 387 Size: 5239 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5239 NotI GC'GGCC_GC Cuts at: 2686 2686 Size: 5239 NspI r_CATG'y Cuts at: 1132 1229 2597 4142 1132 Size: 97 1368 1545 2229 Fragments arranged by size: 2229 1545 1368 97 PasI CC'CwG_GG Cuts at: 1348 2081 2097 1348 Size: 733 16 4490 Fragments arranged by size: 4490 733 16 PciI A'CATG_T Cuts at: 4138 4138 Size: 5239 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5239 PmeI GTTT'AAAC Cuts at: 2543 2543 Size: 5239 PmlI CAC'GTG Cuts at: 2146 2146 Size: 5239 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3308 3340 4821 4853 3308 Size: 32 1481 32 3694 Fragments arranged by size: 3694 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2347 2347 Size: 5239 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5239 PsiI TTA'TAA Cuts at: 2809 2809 Size: 5239 PspOMI G'GGCC_C Cuts at: 2308 2308 Size: 5239 PstI C_TGCA'G Cuts at: 830 2324 2591 830 Size: 1494 267 3478 Fragments arranged by size: 3478 1494 267 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5239 SacII CC_GC'GG Cuts at: 2512 2512 Size: 5239 SalI G'TCGA_C Cuts at: 1936 2581 1936 Size: 645 4594 SbfI CC_TGCA'GG Cuts at: 2591 2591 Size: 5239 ScaI AGT'ACT Cuts at: 3487 5199 3487 Size: 1712 3527 SfcI C'TryA_G Cuts at: 826 1045 2320 2587 3722 4403 4594 826 Size: 219 1275 267 1135 681 191 1471 Fragments arranged by size: 1471 1275 1135 681 267 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5239 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5239 SmaI CCC'GGG Cuts at: 1872 2400 2566 1872 Size: 528 166 4545 Fragments arranged by size: 4545 528 166 SmlI C'TyrA_G Cuts at: 820 1017 1943 3343 4244 4506 820 Size: 197 926 1400 901 262 1553 Fragments arranged by size: 1553 1400 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3030 493 Size: 2537 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5239 SphI G_CATG'C Cuts at: 1132 2597 1132 Size: 1465 3774 SspI AAT'ATT Cuts at: 5 52 3163 5 Size: 47 3111 2081 Fragments arranged by size: 3111 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5239 StyI C'CwwG_G Cuts at: 513 1065 2223 2678 513 Size: 552 1158 455 3074 Fragments arranged by size: 3074 1158 552 455 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2284 2448 3266 3283 3436 3621 4066 Size: 1255 704 164 818 17 153 185 445 Cuts at: 4066 325 Size: 1498 Fragments arranged by size: 1498 1255 818 704 445 185 164 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3485 5197 96 Size: 274 80 33 51 1443 1508 1712 138 Fragments arranged by size: 1712 1508 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5199 5231 5199 Size: 32 5207 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5239 XbaI T'CTAG_A Cuts at: 2575 2575 Size: 5239 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5239 XmaI C'CCGG_G Cuts at: 1870 2398 2564 1870 Size: 528 166 4545 Fragments arranged by size: 4545 528 166 XmnI GAAnn'nnTTC Cuts at: 3368 3368 Size: 5239 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2422 3047 276 Size: 53 83 186 936 888 625 2468 Fragments arranged by size: 2468 936 888 625 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI