################################## # NO CUT ENZYMES ( 106 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Aor51HI ApoI AscI AspEI AspI AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BseAI BsiWI BsmI BspDI BspEI BspLU11I BssHII BssSI Bst1107I BstBI BstYI BstZ17I CelII ClaI CpoI DraI DrdI Eam1105I Eco47III EcoRI EcoT22I FbaI FseI HgaI HincII HindII HindIII HpaI MaeII MamI MfeI MflI MluI MroI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspI StuI SwaI Tsp509I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### Acc65I 788 GGTACC G^GTACC AcyI 926 GGCGCC G[GA]^CG[CT]C Alw44I 508 GTGCAC G^TGCAC AlwI 983 GGATC GGATC(4/5) ApaLI 508 GTGCAC G^TGCAC AseI 1299 ATTAAT AT^TAAT AsnI 1299 ATTAAT AT^TAAT Asp700I 123 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 788 GGTACC G^GTACC AviII 548 TGCGCA TGC^GCA BbeI 926 GGCGCC GGCGC^C BbsI 378 GAAGAC GAAGAC(2/6) BfaI 658 CTAG C^TAG BpuAI 378 GAAGAC GAAGAC(2/6) BsaHI 926 GGCGCC G[GA]^CG[CT]C BsaWI 272 TCCGGT [AT]^CCGG[AT] BsgI 456 CTGCAC GTGCAG(16/14) BsiEI 648 CGGCCG CG[GA][CT]^CG BsmBI 309 CGTCTC CGTCTC(1/5) Bsp1407I 242 TGTACA T^GTACA BspHI 1265 TCATGA T^CATGA BsrBI 599 CCGCTC CCGCTC(-3/-3) BsrDI 1146 CATTGC GCAATG(2/0) BsrGI 242 TGTACA T^GTACA BstEII 1086 GGTGACC G^GT[ACGT]ACC BstPI 1086 GGTGACC G^GT[ACGT]ACC DpnII 984 GATC ^GATC DraIII 1243 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 648 CGGCCG C^GGCCG EclXI 648 CGGCCG C^GGCCG Eco52I 648 CGGCCG C^GGCCG EcoNI 135 CCTGGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1086 GGTGACC G^GT[ACGT]ACC EcoRV 418 GATATC GAT^ATC FspI 548 TGCGCA TGC^GCA Hin1I 926 GGCGCC G[GA]^CG[CT]C KasI 926 GGCGCC G^GCGCC KpnI 788 GGTACC GGTAC^C KspI 796 CCGCGG CCGC^GG MaeI 658 CTAG C^TAG MboI 984 GATC ^GATC MseI 1300 TTAA T^TAA NaeI 646 GCCGGC GCC^GGC NarI 926 GGCGCC GG^CGCC NcoI 197 CCATGG C^CATGG NdeII 984 GATC ^GATC NgoMIV 646 GCCGGC G^CCGGC NspI 1104 GCATGC [GA]CATG^[CT] PpuMI 814 GGGACCC [GA]G^G[AT]CC[CT] PshBI 1299 ATTAAT AT^TAAT PvuII 189 CAGCTG CAG^CTG RcaI 1265 TCATGA T^CATGA SacI 757 GAGCTC GAGCT^C SacII 796 CCGCGG CCGC^GG SapI 126 GCTCTTC GCTCTTC(1/4) Sau3AI 984 GATC ^GATC SfoI 926 GGCGCC GGC^GCC SphI 1104 GCATGC GCATG^C SspBI 242 TGTACA T^GTACA Tru9I 1300 TTAA T^TAA XcmI 1064 CCACGGCGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 123 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccII 797 CGCG CG^CG 1246 CGCG AocI 32 CCTCAGG CC^T[ACGT]AGG 961 CCTGAGG ApaI 66 GGGCCC GGGCC^C 498 GGGCCC AvaI 166 CTCGGG C^[CT]CG[GA]G 751 CCCGAG AvaII 222 GGTCC G^G[AT]CC 815 GGACC BcnI 369 CCCGG CC^[GC]GG 884 CCGGG BsmAI 940 GAGAC GTCTC(1/5) 310 GTCTC BsoBI 166 CTCGGG C^[CT]CG[GA]G 751 CCCGAG BspMI 266 ACCTGC ACCTGC(4/8) 1208 ACCTGC BsrFI 646 GCCGGC [GA]^CCGG[CT] 1090 ACCGGC BstAPI 1021 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1104 GCATGCACTGC BstUI 797 CGCG CG^CG 1246 CGCG BstXI 197 CCATGGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 437 CCAAAGTGGTGG Bsu36I 32 CCTCAGG CC^T[ACGT]AGG 961 CCTGAGG Cfr10I 646 GCCGGC [GA]^CCGG[CT] 1090 ACCGGC Eco81I 32 CCTCAGG CC^T[ACGT]AGG 961 CCTGAGG MslI 870 CATCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1296 CACATTAATG MspA1I 189 CAGCTG C[AC]G^C[GT]G 796 CCGCGG MvnI 797 CGCG CG^CG 1246 CGCG NciI 369 CCCGG CC^[GC]GG 884 CCGGG PstI 52 CTGCAG CTGCA^G 1028 CTGCAG SfcI 52 CTGCAG C^T[GA][CT]AG 1028 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AciI AfaI AluI AlwNI AspHI BalI BanI BanII BbvI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboII MluNI MnlI MscI MspI MvaI MwoI NlaIII NlaIV PleI RsaI Sau96I ScrFI SfaNI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I