(Circular) (Six-base) MAPSORT of: pFN21AE5575.seq Check: 7895 from: 1 to: 6091 pFN21AE5575 6091 bp With 182 enzymes: SgfI * January 30, 2014 17:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2280 2280 Size: 6091 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3901 278 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3434 1937 Size: 1497 4594 Acc65I G'GTAC_C Cuts at: 2793 3412 2793 Size: 619 5472 AclI AA'CG_TT Cuts at: 1563 4218 4591 1563 Size: 2655 373 3063 Fragments arranged by size: 3063 2655 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2141 4154 5538 5806 1632 Size: 509 2013 1384 268 1917 Fragments arranged by size: 2013 1917 1384 509 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5894 AflIII A'CryG_T Cuts at: 1203 4990 5967 1203 Size: 3787 977 1327 Fragments arranged by size: 3787 1327 977 AgeI A'CCGG_T Cuts at: 4922 4922 Size: 6091 AhdI GACnn_n'nnGTC Cuts at: 4820 4820 Size: 6091 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6091 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3178 1184 Size: 1994 4097 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2687 2719 2687 Size: 32 6059 AlwNI CAG_nnn'CTG Cuts at: 2065 2245 2692 2779 3032 3050 3834 5406 2065 Size: 180 447 87 253 18 784 1572 2750 Fragments arranged by size: 2750 1572 784 447 253 180 87 18 ApaI G_GGCC'C Cuts at: 2075 2507 2075 Size: 432 5659 ApaLI G'TGCA_C Cuts at: 2513 4148 5304 2513 Size: 1635 1156 3300 Fragments arranged by size: 3300 1635 1156 ApoI r'AATT_y Cuts at: 1493 3400 3626 3778 5887 1493 Size: 1907 226 152 2109 1697 Fragments arranged by size: 2109 1907 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 3305 4645 160 Size: 872 2273 1340 1606 Fragments arranged by size: 2273 1606 1340 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 AvaI C'yCGr_G Cuts at: 1870 1943 2171 2756 3416 1870 Size: 73 228 585 660 4545 Fragments arranged by size: 4545 660 585 228 73 BamHI G'GATC_C Cuts at: 3421 3788 3421 Size: 367 5724 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2793 2931 3185 3412 4867 Size: 325 217 627 1006 138 254 227 1455 Cuts at: 4867 618 Size: 1842 Fragments arranged by size: 1842 1455 1006 627 325 254 227 217 138 BanII G_rGCy'C Cuts at: 1391 2075 2507 2766 3410 1391 Size: 684 432 259 644 4072 Fragments arranged by size: 4072 684 644 432 259 BbeI G_GCGC'C Cuts at: 2935 2935 Size: 6091 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2390 928 Size: 971 491 4629 Fragments arranged by size: 4629 971 491 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4282 4316 1903 Size: 34 2345 34 3678 Fragments arranged by size: 3678 2345 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3983 5193 3983 Size: 1210 4881 BclI T'GATC_A Cuts at: 1548 1548 Size: 6091 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4702 136 Size: 107 122 71 631 605 3030 1525 Fragments arranged by size: 3030 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6086 6086 Size: 6091 BlpI GC'TnA_GC Cuts at: 3508 3508 Size: 6091 Bme1580I G_kGCm'C Cuts at: 1790 2075 2507 2517 4152 5308 1790 Size: 285 432 10 1635 1156 2573 Fragments arranged by size: 2573 1635 1156 432 285 10 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6091 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3371 3822 4780 Size: 416 53 83 399 24 1954 451 958 Cuts at: 4780 442 Size: 1753 Fragments arranged by size: 1954 1753 958 451 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6091 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2968 2968 Size: 6091 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2132 2379 2725 3003 4751 1239 Size: 893 247 346 278 1748 2579 Fragments arranged by size: 2579 1748 893 346 278 247 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4216 5081 5379 4216 Size: 865 298 4928 Fragments arranged by size: 4928 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4754 882 Size: 617 3255 2219 Fragments arranged by size: 3255 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3882 493 Size: 711 2678 2702 Fragments arranged by size: 2702 2678 711 BsaBI GATnn'nnATC Cuts at: 6085 6085 Size: 6091 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2932 3898 4280 4915 Size: 53 83 186 936 1399 966 382 635 Cuts at: 4915 275 Size: 1451 Fragments arranged by size: 1451 1399 966 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2277 4524 4922 5196 5343 5754 2277 Size: 2247 398 274 147 411 2614 Fragments arranged by size: 2614 2247 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2699 2729 3050 3080 1178 Size: 30 165 30 1296 30 321 30 4189 Fragments arranged by size: 4189 1296 321 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2024 2026 2344 2774 1186 Size: 838 2 318 430 4503 Fragments arranged by size: 4503 838 430 318 2 BseYI C'CCAG_C Cuts at: 2645 2772 5294 5790 2645 Size: 127 2522 496 2946 Fragments arranged by size: 2946 2522 496 127 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2446 1163 Size: 573 710 4808 Fragments arranged by size: 4808 710 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2656 3541 4302 5330 1540 Size: 275 186 655 885 761 1028 2301 Fragments arranged by size: 2301 1028 885 761 655 275 186 BsiHKAI G_wGCw'C Cuts at: 2181 2517 2766 3267 4152 4237 5308 2181 Size: 336 249 501 885 85 1071 2964 Fragments arranged by size: 2964 1071 885 501 336 249 85 BsmI GAATG_Cn' Cuts at: 3602 3695 3602 Size: 93 5998 BsmBI CGTCTCn'nnnn_ Cuts at: 2320 2320 Size: 6091 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2075 2181 2507 2517 2766 3267 3410 Size: 399 285 106 326 10 249 501 143 Cuts at: 3410 4152 4237 5308 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 501 399 326 285 249 143 106 85 10 BspEI T'CCGG_A Cuts at: 5754 5754 Size: 6091 BspHI T'CATG_A Cuts at: 3270 3980 5710 3270 Size: 710 1730 3651 Fragments arranged by size: 3651 1730 710 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2280 3222 3446 844 Size: 1436 942 224 3489 Fragments arranged by size: 3489 1436 942 224 BsrBI CCG'CTC Cuts at: 1394 1823 2606 3978 1394 Size: 429 783 1372 3507 Fragments arranged by size: 3507 1372 783 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3150 4586 4760 65 Size: 1183 1902 1436 174 1396 Fragments arranged by size: 1902 1436 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2651 3095 4735 4922 1700 Size: 110 841 444 1640 187 2869 Fragments arranged by size: 2869 1640 841 444 187 110 BsrGI T'GTAC_A Cuts at: 96 2247 96 Size: 2151 3940 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6091 BssSI C'ACGA_G Cuts at: 4151 5163 4151 Size: 1012 5079 BstAPI GCAn_nnn'nTGC Cuts at: 794 3032 3115 794 Size: 2238 83 3770 Fragments arranged by size: 3770 2238 83 BstBI TT'CG_AA Cuts at: 4895 4895 Size: 6091 BstEII G'GTnAC_C Cuts at: 1184 3091 1184 Size: 1907 4184 BstXI CCAn_nnnn'nTGG Cuts at: 2209 2449 2209 Size: 240 5851 BstYI r'GATC_y Cuts at: 1972 3421 3788 4173 4190 5642 5728 5740 6086 Size: 1449 367 385 17 1452 86 12 346 Cuts at: 6086 1972 Size: 1977 Fragments arranged by size: 1977 1452 1449 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2038 2967 2038 Size: 929 5162 BtgI C'CryG_G Cuts at: 513 1065 2004 2202 2801 3069 3218 513 Size: 552 939 198 599 268 149 3386 Fragments arranged by size: 3386 939 599 552 268 198 149 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5352 Fragments arranged by size: 5352 683 56 BtsI GCAGTG_nn' Cuts at: 3113 3607 4401 4421 3113 Size: 494 794 20 4783 Fragments arranged by size: 4783 794 494 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6091 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6056 DraI TTT'AAA Cuts at: 3395 3395 Size: 6091 DraIII CAC_nnn'GTG Cuts at: 3253 3253 Size: 6091 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5098 809 Size: 507 3782 1802 Fragments arranged by size: 3782 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2610 2653 2865 Size: 54 1425 50 275 18 780 43 212 Cuts at: 2865 3097 3294 3538 4427 6056 8 Size: 232 197 244 889 1629 43 Fragments arranged by size: 1629 1425 889 780 275 244 232 212 197 54 50 43 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2653 3538 1537 Size: 275 841 885 4090 Fragments arranged by size: 4090 885 841 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2138 2329 2492 4021 1353 Size: 785 191 163 1529 3423 Fragments arranged by size: 3423 1529 785 191 163 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2078 2126 4678 5052 5198 387 Size: 1064 138 489 48 2552 374 146 1280 Fragments arranged by size: 2552 1280 1064 489 374 146 138 48 EcoICRI GAG'CTC Cuts at: 2764 2764 Size: 6091 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2132 2141 2379 2725 3003 4154 4751 Size: 393 500 9 238 346 278 1151 597 Cuts at: 4751 5538 5806 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1151 787 597 500 393 346 278 268 238 9 EcoNI CCTnn'n_nnAGG Cuts at: 2144 2144 Size: 6091 EcoO109I rG'GnC_Cy Cuts at: 2071 2072 2504 2820 2071 Size: 1 432 316 5342 Fragments arranged by size: 5342 432 316 1 EcoRI G'AATT_C Cuts at: 3400 3778 3400 Size: 378 5713 EcoRV GAT'ATC Cuts at: 2425 2425 Size: 6091 FspI TGC'GCA Cuts at: 2555 4597 2555 Size: 2042 4049 FspAI rTGC'GCAy Cuts at: 2555 2555 Size: 6091 HaeII r_GCGC'y Cuts at: 1678 2652 2726 2935 3077 5238 1678 Size: 974 74 209 142 2161 2531 Fragments arranged by size: 2531 2161 974 209 142 74 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2699 2731 2910 2942 2980 Size: 32 135 32 1127 32 179 32 38 Cuts at: 2980 3012 4732 4764 4806 4838 1373 Size: 32 1720 32 42 32 2626 Fragments arranged by size: 2626 1720 1127 179 135 42 38 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3435 3681 669 Size: 988 281 1497 246 3079 Fragments arranged by size: 3079 1497 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6091 HpaI GTT'AAC Cuts at: 3681 3681 Size: 6091 KasI G'GCGC_C Cuts at: 2931 2931 Size: 6091 KpnI G_GTAC'C Cuts at: 2797 3416 2797 Size: 619 5472 MfeI C'AATT_G Cuts at: 3690 3690 Size: 6091 MluI A'CGCG_T Cuts at: 5967 5967 Size: 6091 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5205 5389 1305 Size: 3900 184 2007 Fragments arranged by size: 3900 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2612 2867 3296 10 Size: 54 1425 343 780 255 429 2805 Fragments arranged by size: 2805 1425 780 429 343 255 54 MslI CAynn'nnrTG Cuts at: 518 1166 2879 3305 4049 4408 4567 518 Size: 648 1713 426 744 359 159 2042 Fragments arranged by size: 2042 1713 744 648 426 359 159 MspA1I CmG'CkG Cuts at: 1606 2196 2803 3507 4184 5332 5577 1606 Size: 590 607 704 677 1148 245 2120 Fragments arranged by size: 2120 1148 704 677 607 590 245 NaeI GCC'GGC Cuts at: 2653 2653 Size: 6091 NarI GG'CG_CC Cuts at: 2932 2932 Size: 6091 NcoI C'CATG_G Cuts at: 513 1065 2004 2202 513 Size: 552 939 198 4402 Fragments arranged by size: 4402 939 552 198 NdeI CA'TA_TG Cuts at: 387 387 Size: 6091 NgoMIV G'CCGG_C Cuts at: 2651 2651 Size: 6091 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6091 NotI GC'GGCC_GC Cuts at: 3538 3538 Size: 6091 NspI r_CATG'y Cuts at: 1132 1229 3113 3449 4994 1132 Size: 97 1884 336 1545 2229 Fragments arranged by size: 2229 1884 1545 336 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6091 PciI A'CATG_T Cuts at: 4990 4990 Size: 6091 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6091 PfoI T'CCnGG_A Cuts at: 2288 2373 2288 Size: 85 6006 PmeI GTTT'AAAC Cuts at: 3395 3395 Size: 6091 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4160 4192 5673 5705 4160 Size: 32 1481 32 4546 Fragments arranged by size: 4546 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2820 2820 Size: 6091 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6091 PsiI TTA'TAA Cuts at: 3661 3661 Size: 6091 PspOMI G'GGCC_C Cuts at: 2071 2503 2071 Size: 432 5659 PstI C_TGCA'G Cuts at: 830 2061 3037 3443 830 Size: 1231 976 406 3478 Fragments arranged by size: 3478 1231 976 406 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6091 PvuII CAG'CTG Cuts at: 2196 2196 Size: 6091 SacI G_AGCT'C Cuts at: 2766 2766 Size: 6091 SacII CC_GC'GG Cuts at: 2804 2804 Size: 6091 SalI G'TCGA_C Cuts at: 1936 3433 1936 Size: 1497 4594 SanDI GG'GwC_CC Cuts at: 2820 2820 Size: 6091 SapI GCTCTTCn'nnn_ Cuts at: 2138 2138 Size: 6091 SbfI CC_TGCA'GG Cuts at: 3443 3443 Size: 6091 ScaI AGT'ACT Cuts at: 4339 6051 4339 Size: 1712 4379 SfcI C'TryA_G Cuts at: 826 1045 2057 3033 3439 4574 5255 5446 826 Size: 219 1012 976 406 1135 681 191 1471 Fragments arranged by size: 1471 1135 1012 976 681 406 219 191 SfoI GGC'GCC Cuts at: 2933 2933 Size: 6091 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6091 SmaI CCC'GGG Cuts at: 1872 3418 1872 Size: 1546 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4195 5096 5358 820 Size: 197 926 2252 901 262 1553 Fragments arranged by size: 2252 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3882 493 Size: 3389 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6091 SphI G_CATG'C Cuts at: 1132 3113 3449 1132 Size: 1981 336 3774 Fragments arranged by size: 3774 1981 336 SspI AAT'ATT Cuts at: 5 52 4015 5 Size: 47 3963 2081 Fragments arranged by size: 3963 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6091 StyI C'CwwG_G Cuts at: 513 1065 2004 2202 2616 3337 3530 513 Size: 552 939 198 414 721 193 3074 Fragments arranged by size: 3074 939 721 552 414 198 193 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2659 4118 4135 4288 4473 4918 325 Size: 1255 1079 1459 17 153 185 445 1498 Fragments arranged by size: 1498 1459 1255 1079 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2247 2598 3325 Size: 274 80 33 51 1443 270 351 727 Cuts at: 3325 4337 6049 96 Size: 1012 1712 138 Fragments arranged by size: 1712 1443 1012 727 351 274 270 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3050 3082 6051 6083 3050 Size: 32 2969 32 3058 Fragments arranged by size: 3058 2969 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6091 XbaI T'CTAG_A Cuts at: 3427 3427 Size: 6091 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3076 3076 Size: 6091 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6091 XmaI C'CCGG_G Cuts at: 1870 3416 1870 Size: 1546 4545 XmnI GAAnn'nnTTC Cuts at: 2132 4220 2132 Size: 2088 4003 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3899 276 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I FalI FseI NruI NsiI PacI PmlI PspXI PsrI RsrII SexAI SfiI SrfI SwaI