(Linear) (Six-base) MAPSORT of: KIEE5575.seq Check: 4389 from: 1 to: 1386 KIEE5575.orf With 182 enzymes: SgfI * January 29, 2014 12:06 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 275 1386 Size: 275 1111 Acc65I G'GTAC_C Cuts at: 0 788 1386 Size: 788 598 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 136 1386 Size: 136 1250 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1173 1386 Size: 1173 213 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 682 714 1386 Size: 682 32 672 Fragments arranged by size: 682 672 32 AlwNI CAG_nnn'CTG Cuts at: 0 60 240 687 774 1027 1045 1386 Size: 60 180 447 87 253 18 341 Fragments arranged by size: 447 341 253 180 87 60 18 ApaI G_GGCC'C Cuts at: 0 70 502 1386 Size: 70 432 884 Fragments arranged by size: 884 432 70 ApaLI G'TGCA_C Cuts at: 0 508 1386 Size: 508 878 AseI AT'TA_AT Cuts at: 0 1300 1386 Size: 1300 86 AvaI C'yCGr_G Cuts at: 0 166 751 1386 Size: 166 585 635 Fragments arranged by size: 635 585 166 BanI G'GyrC_C Cuts at: 0 788 926 1180 1386 Size: 788 138 254 206 Fragments arranged by size: 788 254 206 138 BanII G_rGCy'C Cuts at: 0 70 502 761 1386 Size: 70 432 259 625 Fragments arranged by size: 625 432 259 70 BbeI G_GCGC'C Cuts at: 0 930 1386 Size: 930 456 BbsI GAAGACnn'nnnn_ Cuts at: 0 385 1386 Size: 385 1001 Bme1580I G_kGCm'C Cuts at: 0 70 502 512 1386 Size: 70 432 10 874 Fragments arranged by size: 874 432 70 10 BmrI ACTGGGnnnn_n' Cuts at: 0 1366 1386 Size: 1366 20 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 963 1386 Size: 963 423 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 127 374 720 998 1386 Size: 127 247 346 278 388 Fragments arranged by size: 388 346 278 247 127 BsaHI Gr'CG_yC Cuts at: 0 927 1386 Size: 927 459 BsaWI w'CCGG_w Cuts at: 0 272 1386 Size: 272 1114 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 694 724 1045 1075 1386 Size: 694 30 321 30 311 Fragments arranged by size: 694 321 311 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 19 21 339 769 1386 Size: 19 2 318 430 617 Fragments arranged by size: 617 430 318 19 2 BseYI C'CCAG_C Cuts at: 0 640 767 1386 Size: 640 127 619 Fragments arranged by size: 640 619 127 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 441 1386 Size: 441 945 BsiEI CG_ry'CG Cuts at: 0 651 1386 Size: 651 735 BsiHKAI G_wGCw'C Cuts at: 0 176 512 761 1262 1386 Size: 176 336 249 501 124 Fragments arranged by size: 501 336 249 176 124 BsmBI CGTCTCn'nnnn_ Cuts at: 0 315 1386 Size: 315 1071 Bsp1286I G_dGCh'C Cuts at: 0 70 176 502 512 761 1262 1386 Size: 70 106 326 10 249 501 124 Fragments arranged by size: 501 326 249 124 106 70 10 BspHI T'CATG_A Cuts at: 0 1265 1386 Size: 1265 121 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 275 1217 1386 Size: 275 942 169 Fragments arranged by size: 942 275 169 BsrBI CCG'CTC Cuts at: 0 601 1386 Size: 601 785 BsrDI GCAATG_nn' Cuts at: 0 1145 1386 Size: 1145 241 BsrFI r'CCGG_y Cuts at: 0 646 1090 1386 Size: 646 444 296 Fragments arranged by size: 646 444 296 BsrGI T'GTAC_A Cuts at: 0 242 1386 Size: 242 1144 BstAPI GCAn_nnn'nTGC Cuts at: 0 1027 1110 1386 Size: 1027 83 276 Fragments arranged by size: 1027 276 83 BstEII G'GTnAC_C Cuts at: 0 1086 1386 Size: 1086 300 BstXI CCAn_nnnn'nTGG Cuts at: 0 204 444 1386 Size: 204 240 942 Fragments arranged by size: 942 240 204 Bsu36I CC'TnA_GG Cuts at: 0 33 962 1386 Size: 33 929 424 Fragments arranged by size: 929 424 33 BtgI C'CryG_G Cuts at: 0 197 796 1064 1213 1386 Size: 197 599 268 149 173 Fragments arranged by size: 599 268 197 173 149 BtsI GCAGTG_nn' Cuts at: 0 1108 1386 Size: 1108 278 DraIII CAC_nnn'GTG Cuts at: 0 1248 1386 Size: 1248 138 EaeI y'GGCC_r Cuts at: 0 605 648 860 1092 1289 1386 Size: 605 43 212 232 197 97 Fragments arranged by size: 605 232 212 197 97 43 EagI C'GGCC_G Cuts at: 0 648 1386 Size: 648 738 EarI CTCTTCn'nnn_ Cuts at: 0 133 324 487 1386 Size: 133 191 163 899 Fragments arranged by size: 899 191 163 133 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 73 121 1386 Size: 73 48 1265 Fragments arranged by size: 1265 73 48 EcoICRI GAG'CTC Cuts at: 0 759 1386 Size: 759 627 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 127 136 374 720 998 1386 Size: 127 9 238 346 278 388 Fragments arranged by size: 388 346 278 238 127 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 139 1386 Size: 139 1247 EcoO109I rG'GnC_Cy Cuts at: 0 66 67 499 815 1386 Size: 66 1 432 316 571 Fragments arranged by size: 571 432 316 66 1 EcoRV GAT'ATC Cuts at: 0 420 1386 Size: 420 966 FspI TGC'GCA Cuts at: 0 550 1386 Size: 550 836 FspAI rTGC'GCAy Cuts at: 0 550 1386 Size: 550 836 HaeII r_GCGC'y Cuts at: 0 647 721 930 1072 1386 Size: 647 74 209 142 314 Fragments arranged by size: 647 314 209 142 74 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 694 726 905 937 975 1007 1386 Size: 694 32 179 32 38 32 379 Fragments arranged by size: 694 379 179 38 32 32 32 KasI G'GCGC_C Cuts at: 0 926 1386 Size: 926 460 KpnI G_GTAC'C Cuts at: 0 792 1386 Size: 792 594 MscI TGG'CCA Cuts at: 0 607 862 1291 1386 Size: 607 255 429 95 Fragments arranged by size: 607 429 255 95 MslI CAynn'nnrTG Cuts at: 0 874 1300 1386 Size: 874 426 86 Fragments arranged by size: 874 426 86 MspA1I CmG'CkG Cuts at: 0 191 798 1386 Size: 191 607 588 Fragments arranged by size: 607 588 191 NaeI GCC'GGC Cuts at: 0 648 1386 Size: 648 738 NarI GG'CG_CC Cuts at: 0 927 1386 Size: 927 459 NcoI C'CATG_G Cuts at: 0 197 1386 Size: 197 1189 NgoMIV G'CCGG_C Cuts at: 0 646 1386 Size: 646 740 NspI r_CATG'y Cuts at: 0 1108 1386 Size: 1108 278 PfoI T'CCnGG_A Cuts at: 0 283 368 1386 Size: 283 85 1018 Fragments arranged by size: 1018 283 85 PpuMI rG'GwC_Cy Cuts at: 0 815 1386 Size: 815 571 PspOMI G'GGCC_C Cuts at: 0 66 498 1386 Size: 66 432 888 Fragments arranged by size: 888 432 66 PstI C_TGCA'G Cuts at: 0 56 1032 1386 Size: 56 976 354 Fragments arranged by size: 976 354 56 PvuII CAG'CTG Cuts at: 0 191 1386 Size: 191 1195 SacI G_AGCT'C Cuts at: 0 761 1386 Size: 761 625 SacII CC_GC'GG Cuts at: 0 799 1386 Size: 799 587 SanDI GG'GwC_CC Cuts at: 0 815 1386 Size: 815 571 SapI GCTCTTCn'nnn_ Cuts at: 0 133 1386 Size: 133 1253 SfcI C'TryA_G Cuts at: 0 52 1028 1386 Size: 52 976 358 Fragments arranged by size: 976 358 52 SfoI GGC'GCC Cuts at: 0 928 1386 Size: 928 458 SphI G_CATG'C Cuts at: 0 1108 1386 Size: 1108 278 StyI C'CwwG_G Cuts at: 0 197 611 1332 1386 Size: 197 414 721 54 Fragments arranged by size: 721 414 197 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 654 1386 Size: 654 732 TatI w'GTAC_w Cuts at: 0 242 593 1320 1386 Size: 242 351 727 66 Fragments arranged by size: 727 351 242 66 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1045 1077 1386 Size: 1045 32 309 Fragments arranged by size: 1045 309 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1071 1386 Size: 1071 315 XmnI GAAnn'nnTTC Cuts at: 0 127 1386 Size: 127 1259 Enzymes that do cut: AarI Acc65I AcuI AlfI AloI AlwNI ApaI ApaLI AseI AvaI BanI BanII BbeI BbsI Bme1580I BmrI BplI BpmI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstEII BstXI Bsu36I BtgI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FspI FspAI HaeII Hin4I KasI KpnI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PfoI PpuMI PspOMI PstI PvuII SacI SacII SanDI SapI SfcI SfoI SphI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AatII AccI AclI AfeI AflII AflIII AgeI AhdI AleI ApoI AscI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI Bpu10I BpuEI BsaI BsaAI BsaBI BsiWI BsmI BspEI BssHII BssSI BstBI BstYI BstZ17I BtgZI ClaI CspCI DraI DrdI EcoRI FalI FseI HincII HindIII HpaI MfeI MluI MmeI NdeI NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI ZraI