Restriction map for
pFN21AE5686
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI AspEI AspHI
AvrII BamHI BbeI BbrPI BbsI
BcgI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BseAI BsgI
BsiHKAI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRV FseI HincII HindII
HpaI KasI KspI MfeI MflI
MluI MroI MunI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tsp509I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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Acc65I 506 GGTACC G^GTACC
AflIII 302 ACACGT A^C[GA][CT]GT
AlwNI 172 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 809 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 506 GGTACC G^GTACC
AspI 1348 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 728 TGCGCA TGC^GCA
BciVI 565 GGATAC GTATCC(6/5)
BsaBI 1665 GATAGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 903 CGACCG CG[GA][CT]^CG
BsmI 347 GCATTC GAATGC(1/-1)
BsrBI 892 GAGCGG CCGCTC(-3/-3)
BstEII 43 GGTAACC G^GT[ACGT]ACC
BstPI 43 GGTAACC G^GT[ACGT]ACC
DraIII 1657 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 951 GACCAAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 43 GGTAACC G^GT[ACGT]ACC
EcoT22I 1228 ATGCAT ATGCA^T
FspI 728 TGCGCA TGC^GCA
HaeII 1319 AGCGCC [GA]GCGC^[CT]
HindIII 1342 AAGCTT A^AGCTT
KpnI 506 GGTACC GGTAC^C
MamI 1665 GATAGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 239 TTAA T^TAA
NdeI 848 CATATG CA^TATG
NsiI 1228 ATGCAT ATGCA^T
NspI 1571 GCATGC [GA]CATG^[CT]
PflFI 1348 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 294 GGGACCT [GA]G^G[AT]CC[CT]
SapI 1114 GAAGAGC GCTCTTC(1/4)
SphI 1571 GCATGC GCATG^C
StuI 127 AGGCCT AGG^CCT
TfiI 1146 GAATC G^A[AT]TC
Tru9I 239 TTAA T^TAA
Tth111I 1348 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 809 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 26 CGCG CG^CG
475 CGCG
AcyI 589 GACGCC G[GA]^CG[CT]C
612 GACGCC
AvaI 4 CCCGAG C^[CT]CG[GA]G
323 CCCGAG
BalI 138 TGGCCA TGG^CCA
203 TGGCCA
BbvCI 975 CCTCAGC CCTCAGC(-5/-2)
997 GCTGAGG
BcnI 572 CCCGG CC^[GC]GG
1459 CCCGG
BfaI 1237 CTAG C^TAG
1645 CTAG
BpmI 939 CTCCAG CTGGAG(16/14)
1615 CTCCAG
BsaHI 589 GACGCC G[GA]^CG[CT]C
612 GACGCC
BsaWI 644 ACCGGA [AT]^CCGG[AT]
1082 ACCGGA
BsmBI 274 CGTCTC CGTCTC(1/5)
1559 GAGACG
BsoBI 4 CCCGAG C^[CT]CG[GA]G
323 CCCGAG
BssSI 1027 CACGAG CACGAG(-5/-1)
1126 CACGAG
BstF5I 9 GGATG GGATG(2/0)
1442 GGATG
BstUI 26 CGCG CG^CG
475 CGCG
BstXI 1103 CCAAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1278 CCACACTTCTGG
DraII 82 AGGGCCT [GA]G^G[ACGT]CC[CT]
294 GGGACCT
EaeI 138 TGGCCA [CT]^GGCC[GA]
203 TGGCCA
EcoO109I 82 AGGGCCT [GA]G^G[ACGT]CC[CT]
294 GGGACCT
FokI 9 GGATG GGATG(9/13)
1442 GGATG
Hin1I 589 GACGCC G[GA]^CG[CT]C
612 GACGCC
MaeI 1237 CTAG C^TAG
1645 CTAG
MluNI 138 TGGCCA TGG^CCA
203 TGGCCA
MscI 138 TGGCCA TGG^CCA
203 TGGCCA
MvnI 26 CGCG CG^CG
475 CGCG
NciI 572 CCCGG CC^[GC]GG
1459 CCCGG
PflMI 171 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
779 CCAAGCCCTGG
PstI 1034 CTGCAG CTGCA^G
1465 CTGCAG
SexAI 596 ACCTGGT A^CC[AT]GGT
1487 ACCTGGT
SmlI 163 CTCAAG C^T[CT][GA]AG
706 CTTGAG
Van91I 171 CCAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
779 CCAAGCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AfaI AluI AvaII BanI
BanII BbvI BclI BglI BmyI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EarI EcoRII EcoT14I
FauI FbaI Fnu4HI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TseI Tsp45I TspRI TthHB8I