(Circular) (Six-base) MAPSORT of: pFN21AE5686.seq Check: 2020 from: 1 to: 6394 pFN21AE5686 6394 bp With 182 enzymes: SgfI * December 8, 2014 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4204 278 Size: 53 83 186 936 2668 2468 Fragments arranged by size: 2668 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3737 1937 Size: 1800 4594 Acc65I G'GTAC_C Cuts at: 2511 3715 2511 Size: 1204 5190 AclI AA'CG_TT Cuts at: 1563 4521 4894 1563 Size: 2958 373 3063 Fragments arranged by size: 3063 2958 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3167 3332 4457 5841 6109 1632 Size: 1535 165 1125 1384 268 1917 Fragments arranged by size: 1917 1535 1384 1125 268 165 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6197 AflIII A'CryG_T Cuts at: 1203 2307 5293 6270 1203 Size: 1104 2986 977 1327 Fragments arranged by size: 2986 1327 1104 977 AgeI A'CCGG_T Cuts at: 5225 5225 Size: 6394 AhdI GACnn_n'nnGTC Cuts at: 5123 5123 Size: 6394 AleI CACnn'nnGTG Cuts at: 1166 2946 1166 Size: 1780 4614 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3593 1184 Size: 2409 3985 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3482 3514 3482 Size: 32 6362 AlwNI CAG_nnn'CTG Cuts at: 2182 4137 5709 2182 Size: 1955 1572 2867 Fragments arranged by size: 2867 1955 1572 ApaLI G'TGCA_C Cuts at: 4451 5607 4451 Size: 1156 5238 ApoI r'AATT_y Cuts at: 1493 3703 3929 4081 6190 1493 Size: 2210 226 152 2109 1697 Fragments arranged by size: 2210 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4948 160 Size: 872 3916 1606 Fragments arranged by size: 3916 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6394 AvaI C'yCGr_G Cuts at: 1870 1943 2009 2328 3719 1870 Size: 73 66 319 1391 4545 Fragments arranged by size: 4545 1391 319 73 66 BamHI G'GATC_C Cuts at: 3724 4091 3724 Size: 367 6027 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2153 2511 2580 3715 5170 Size: 325 217 627 366 358 69 1135 1455 Cuts at: 5170 618 Size: 1842 Fragments arranged by size: 1842 1455 1135 627 366 358 325 217 69 BanII G_rGCy'C Cuts at: 1391 2906 3108 3386 3465 3713 1391 Size: 1515 202 278 79 248 4072 Fragments arranged by size: 4072 1515 278 248 202 79 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5423 BbvCI CC'TCA_GC Cuts at: 2981 3003 2981 Size: 22 6372 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4585 4619 1903 Size: 34 50 34 2564 34 3678 Fragments arranged by size: 3678 2564 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2564 4286 5496 2564 Size: 1722 1210 3462 Fragments arranged by size: 3462 1722 1210 BclI T'GATC_A Cuts at: 1548 2365 3261 3531 1548 Size: 817 896 270 4411 Fragments arranged by size: 4411 896 817 270 BfrBI ATG'CAT Cuts at: 3235 3235 Size: 6394 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2198 2715 3390 Size: 107 122 71 631 605 526 517 675 Cuts at: 3390 3416 5005 136 Size: 26 1589 1525 Fragments arranged by size: 1589 1525 675 631 605 526 517 122 107 71 26 BglII A'GATC_T Cuts at: 6389 6389 Size: 6394 BlpI GC'TnA_GC Cuts at: 3811 3811 Size: 6394 Bme1580I G_kGCm'C Cuts at: 1790 4455 5611 1790 Size: 2665 1156 2573 Fragments arranged by size: 2665 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2310 2371 3228 1318 Size: 992 61 857 4484 Fragments arranged by size: 4484 992 857 61 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2676 3420 4125 Size: 416 53 83 399 24 1259 744 705 Cuts at: 4125 5083 442 Size: 958 1753 Fragments arranged by size: 1753 1259 958 744 705 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6394 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2920 2959 2920 Size: 39 6355 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2929 3605 5054 1239 Size: 1690 676 1449 2579 Fragments arranged by size: 2579 1690 1449 676 Bpu10I CC'TnA_GC Cuts at: 2202 2919 2981 3003 2202 Size: 717 62 22 5593 Fragments arranged by size: 5593 717 62 22 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2153 2732 4519 5384 5682 2153 Size: 579 1787 865 298 2865 Fragments arranged by size: 2865 1787 865 579 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2640 2763 3128 3502 5057 882 Size: 617 1141 123 365 374 1555 2219 Fragments arranged by size: 2219 1555 1141 617 374 365 123 BsaAI yAC'GTr Cuts at: 493 1204 4185 493 Size: 711 2981 2702 Fragments arranged by size: 2981 2702 711 BsaBI GATnn'nnATC Cuts at: 3674 6388 3674 Size: 2714 3680 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2595 2618 4201 4583 Size: 53 83 186 936 1062 23 1583 382 Cuts at: 4583 5218 275 Size: 635 1451 Fragments arranged by size: 1583 1451 1062 936 635 382 186 83 53 23 BsaWI w'CCGG_w Cuts at: 2649 3087 4827 5225 5499 5646 6057 2649 Size: 438 1740 398 274 147 411 2986 Fragments arranged by size: 2986 1740 438 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2495 2525 3482 3512 1178 Size: 30 165 30 1092 30 957 30 4060 Fragments arranged by size: 4060 1092 957 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2129 2516 3263 1186 Size: 943 387 747 4317 Fragments arranged by size: 4317 943 747 387 BseYI C'CCAG_C Cuts at: 2517 3181 3306 3321 3609 5597 6093 2517 Size: 664 125 15 288 1988 496 2818 Fragments arranged by size: 2818 1988 664 496 288 125 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5821 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2911 3844 4605 5633 1540 Size: 275 186 910 933 761 1028 2301 Fragments arranged by size: 2301 1028 933 910 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4455 4540 5611 4455 Size: 85 1071 5238 Fragments arranged by size: 5238 1071 85 BsmI GAATG_Cn' Cuts at: 2352 3905 3998 2352 Size: 1553 93 4748 Fragments arranged by size: 4748 1553 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2285 3558 2285 Size: 1273 5121 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2906 3108 3386 3465 3713 4455 4540 Size: 399 1116 202 278 79 248 742 85 Cuts at: 4540 5611 1391 Size: 1071 2174 Fragments arranged by size: 2174 1116 1071 742 399 278 248 202 85 79 BspEI T'CCGG_A Cuts at: 6057 6057 Size: 6394 BspHI T'CATG_A Cuts at: 4283 6013 4283 Size: 1730 4664 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3749 844 Size: 2905 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2899 4281 1394 Size: 429 1076 1382 3507 Fragments arranged by size: 3507 1382 1076 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4889 5063 65 Size: 1183 3641 174 1396 Fragments arranged by size: 3641 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5038 5225 1700 Size: 110 3228 187 2869 Fragments arranged by size: 3228 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6394 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6394 BssSI C'ACGA_G Cuts at: 3032 3131 4454 5466 3032 Size: 99 1323 1012 3960 Fragments arranged by size: 3960 1323 1012 99 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6394 BstBI TT'CG_AA Cuts at: 5198 5198 Size: 6394 BstEII G'GTnAC_C Cuts at: 1184 2048 1184 Size: 864 5530 BstXI CCAn_nnnn'nTGG Cuts at: 3115 3290 3115 Size: 175 6219 BstYI r'GATC_y Cuts at: 1972 3724 4091 4476 4493 5945 6031 6043 6389 Size: 1752 367 385 17 1452 86 12 346 Cuts at: 6389 1972 Size: 1977 Fragments arranged by size: 1977 1752 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2140 2773 2830 3052 513 Size: 552 1075 633 57 222 3855 Fragments arranged by size: 3855 1075 633 552 222 57 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5655 Fragments arranged by size: 5655 683 56 BtsI GCAGTG_nn' Cuts at: 2887 3687 3910 4704 4724 2887 Size: 800 223 794 20 4557 Fragments arranged by size: 4557 800 794 223 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6394 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2863 2898 529 Size: 35 2299 35 4025 Fragments arranged by size: 4025 2299 35 35 DraI TTT'AAA Cuts at: 3698 3698 Size: 6394 DraIII CAC_nnn'GTG Cuts at: 3667 3667 Size: 6394 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2962 5401 809 Size: 507 1646 2439 1802 Fragments arranged by size: 2439 1802 1646 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2143 2208 3841 Size: 54 1425 50 275 18 313 65 1633 Cuts at: 3841 4730 6359 8 Size: 889 1629 43 Fragments arranged by size: 1633 1629 1425 889 313 275 65 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3841 1537 Size: 275 2029 4090 Fragments arranged by size: 4090 2029 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2045 2790 3052 3114 4324 1353 Size: 692 745 262 62 1210 3423 Fragments arranged by size: 3423 1210 745 692 262 62 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4981 5355 5501 387 Size: 1064 138 3392 374 146 1280 Fragments arranged by size: 3392 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2929 3167 3332 3605 4457 5054 5841 Size: 393 1297 238 165 273 852 597 787 Cuts at: 5841 6109 1239 Size: 268 1524 Fragments arranged by size: 1524 1297 852 787 597 393 273 268 238 165 EcoO109I rG'GnC_Cy Cuts at: 2088 2300 2088 Size: 212 6182 EcoRI G'AATT_C Cuts at: 3703 4081 3703 Size: 378 6016 FspI TGC'GCA Cuts at: 2735 4900 2735 Size: 2165 4229 HaeII r_GCGC'y Cuts at: 1678 3328 5541 1678 Size: 1650 2213 2531 Fragments arranged by size: 2531 2213 1650 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2350 2382 3253 3285 5035 Size: 32 135 32 778 32 871 32 1750 Cuts at: 5035 5067 5109 5141 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1750 871 778 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3738 3984 669 Size: 988 281 1800 246 3079 Fragments arranged by size: 3079 1800 988 281 246 HindIII A'AGCT_T Cuts at: 748 3347 748 Size: 2599 3795 HpaI GTT'AAC Cuts at: 3984 3984 Size: 6394 KpnI G_GTAC'C Cuts at: 2515 3719 2515 Size: 1204 5190 MfeI C'AATT_G Cuts at: 3993 3993 Size: 6394 MluI A'CGCG_T Cuts at: 6270 6270 Size: 6394 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2931 3167 5508 5692 1305 Size: 1626 236 2341 184 2007 Fragments arranged by size: 2341 2007 1626 236 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2145 2210 10 Size: 54 1425 343 313 65 4194 Fragments arranged by size: 4194 1425 343 313 65 54 MslI CAynn'nnrTG Cuts at: 518 1166 2946 3230 3575 4352 4711 4870 518 Size: 648 1780 284 345 777 359 159 2042 Fragments arranged by size: 2042 1780 777 648 359 345 284 159 MspA1I CmG'CkG Cuts at: 1606 2023 3181 3216 3310 3810 4487 5635 5880 Size: 417 1158 35 94 500 677 1148 245 Cuts at: 5880 1606 Size: 2120 Fragments arranged by size: 2120 1158 1148 677 500 417 245 94 35 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5842 NdeI CA'TA_TG Cuts at: 387 2854 387 Size: 2467 3927 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6394 NotI GC'GGCC_GC Cuts at: 3841 3841 Size: 6394 NsiI A_TGCA'T Cuts at: 3237 3237 Size: 6394 NspI r_CATG'y Cuts at: 1132 1229 3580 3752 5297 1132 Size: 97 2351 172 1545 2229 Fragments arranged by size: 2351 2229 1545 172 97 PasI CC'CwG_GG Cuts at: 1348 2790 1348 Size: 1442 4952 PciI A'CATG_T Cuts at: 5293 5293 Size: 6394 PflMI CCAn_nnn'nTGG Cuts at: 1108 2182 2790 1108 Size: 1074 608 4712 Fragments arranged by size: 4712 1074 608 PmeI GTTT'AAAC Cuts at: 3698 3698 Size: 6394 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3482 3514 4463 4495 5976 6008 3482 Size: 32 949 32 1481 32 3868 Fragments arranged by size: 3868 1481 949 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2300 2300 Size: 6394 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6394 PsiI TTA'TAA Cuts at: 3964 3964 Size: 6394 PstI C_TGCA'G Cuts at: 830 3043 3474 3746 830 Size: 2213 431 272 3478 Fragments arranged by size: 3478 2213 431 272 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6394 PvuII CAG'CTG Cuts at: 3181 3216 3310 3181 Size: 35 94 6265 Fragments arranged by size: 6265 94 35 SalI G'TCGA_C Cuts at: 1936 3736 1936 Size: 1800 4594 SapI GCTCTTCn'nnn_ Cuts at: 3114 3114 Size: 6394 SbfI CC_TGCA'GG Cuts at: 3746 3746 Size: 6394 ScaI AGT'ACT Cuts at: 4642 6354 4642 Size: 1712 4682 SexAI A'CCwGG_T Cuts at: 2601 3492 2601 Size: 891 5503 SfcI C'TryA_G Cuts at: 826 1045 2719 3039 3176 3470 3742 4877 5558 Size: 219 1674 320 137 294 272 1135 681 Cuts at: 5558 5749 826 Size: 191 1471 Fragments arranged by size: 1674 1471 1135 681 320 294 272 219 191 137 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6394 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6394 SmaI CCC'GGG Cuts at: 1872 3721 1872 Size: 1849 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2168 2711 4498 5399 5661 820 Size: 197 926 225 543 1787 901 262 1553 Fragments arranged by size: 1787 1553 926 901 543 262 225 197 SnaBI TAC'GTA Cuts at: 493 4185 493 Size: 3692 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6394 SphI G_CATG'C Cuts at: 1132 3580 3752 1132 Size: 2448 172 3774 Fragments arranged by size: 3774 2448 172 SspI AAT'ATT Cuts at: 5 52 4318 5 Size: 47 4266 2081 Fragments arranged by size: 4266 2081 47 StuI AGG'CCT Cuts at: 1517 2134 1517 Size: 617 5777 StyI C'CwwG_G Cuts at: 513 1065 2454 2874 2958 3555 3833 513 Size: 552 1389 420 84 597 278 3074 Fragments arranged by size: 3074 1389 597 552 420 278 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2275 2279 2447 2658 3428 4421 4438 Size: 1255 695 4 168 211 770 993 17 Cuts at: 4438 4591 4776 5221 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 993 770 695 445 211 185 168 153 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2634 4640 6352 Size: 274 80 33 51 1443 657 2006 1712 Cuts at: 6352 96 Size: 138 Fragments arranged by size: 2006 1712 1443 657 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6354 6386 6354 Size: 32 6362 Tth111I GACn'n_nGTC Cuts at: 1316 3356 1316 Size: 2040 4354 XbaI T'CTAG_A Cuts at: 3730 3730 Size: 6394 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6394 XmaI C'CCGG_G Cuts at: 1870 3719 1870 Size: 1849 4545 XmnI GAAnn'nnTTC Cuts at: 2818 4523 2818 Size: 1705 4689 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4202 276 Size: 53 83 186 936 2668 2468 Fragments arranged by size: 2668 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI