(Linear) (Six-base) MAPSORT of: KIEE5686.seq Check: 9245 from: 1 to: 1689 KIEE5686.orf With 182 enzymes: SgfI * December 5, 2014 13:06 .. Acc65I G'GTAC_C Cuts at: 0 506 1689 Size: 506 1183 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1162 1327 1689 Size: 1162 165 362 Fragments arranged by size: 1162 362 165 AflIII A'CryG_T Cuts at: 0 302 1689 Size: 302 1387 AleI CACnn'nnGTG Cuts at: 0 941 1689 Size: 941 748 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1588 1689 Size: 1588 101 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1477 1509 1689 Size: 1477 32 180 Fragments arranged by size: 1477 180 32 AlwNI CAG_nnn'CTG Cuts at: 0 177 1689 Size: 177 1512 AvaI C'yCGr_G Cuts at: 0 4 323 1689 Size: 4 319 1366 Fragments arranged by size: 1366 319 4 BanI G'GyrC_C Cuts at: 0 148 506 575 1689 Size: 148 358 69 1114 Fragments arranged by size: 1114 358 148 69 BanII G_rGCy'C Cuts at: 0 901 1103 1381 1460 1689 Size: 901 202 278 79 229 Fragments arranged by size: 901 278 229 202 79 BbvCI CC'TCA_GC Cuts at: 0 976 998 1689 Size: 976 22 691 Fragments arranged by size: 976 691 22 BciVI GTATCCnnnnn_n' Cuts at: 0 559 1689 Size: 559 1130 BclI T'GATC_A Cuts at: 0 360 1256 1526 1689 Size: 360 896 270 163 Fragments arranged by size: 896 360 270 163 BfrBI ATG'CAT Cuts at: 0 1230 1689 Size: 1230 459 BglI GCCn_nnn'nGGC Cuts at: 0 193 710 1385 1411 1689 Size: 193 517 675 26 278 Fragments arranged by size: 675 517 278 193 26 BmgBI CAC'GTC Cuts at: 0 305 366 1223 1689 Size: 305 61 857 466 Fragments arranged by size: 857 466 305 61 BmrI ACTGGGnnnn_n' Cuts at: 0 671 1415 1689 Size: 671 744 274 Fragments arranged by size: 744 671 274 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 915 954 1689 Size: 915 39 735 Fragments arranged by size: 915 735 39 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 924 1600 1689 Size: 924 676 89 Fragments arranged by size: 924 676 89 Bpu10I CC'TnA_GC Cuts at: 0 197 914 976 998 1689 Size: 197 717 62 22 691 Fragments arranged by size: 717 691 197 62 22 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 148 727 1689 Size: 148 579 962 Fragments arranged by size: 962 579 148 BsaI GGTCTCn'nnnn_ Cuts at: 0 635 758 1123 1497 1689 Size: 635 123 365 374 192 Fragments arranged by size: 635 374 365 192 123 BsaBI GATnn'nnATC Cuts at: 0 1669 1689 Size: 1669 20 BsaHI Gr'CG_yC Cuts at: 0 590 613 1689 Size: 590 23 1076 Fragments arranged by size: 1076 590 23 BsaWI w'CCGG_w Cuts at: 0 644 1082 1689 Size: 644 438 607 Fragments arranged by size: 644 607 438 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 490 520 1477 1507 1689 Size: 490 30 957 30 182 Fragments arranged by size: 957 490 182 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 124 511 1258 1689 Size: 124 387 747 431 Fragments arranged by size: 747 431 387 124 BseYI C'CCAG_C Cuts at: 0 512 1176 1301 1316 1604 1689 Size: 512 664 125 15 288 85 Fragments arranged by size: 664 512 288 125 85 15 BsiEI CG_ry'CG Cuts at: 0 906 1689 Size: 906 783 BsmI GAATG_Cn' Cuts at: 0 347 1689 Size: 347 1342 BsmBI CGTCTCn'nnnn_ Cuts at: 0 280 1553 1689 Size: 280 1273 136 Fragments arranged by size: 1273 280 136 Bsp1286I G_dGCh'C Cuts at: 0 901 1103 1381 1460 1689 Size: 901 202 278 79 229 Fragments arranged by size: 901 278 229 202 79 BsrBI CCG'CTC Cuts at: 0 894 1689 Size: 894 795 BssSI C'ACGA_G Cuts at: 0 1027 1126 1689 Size: 1027 99 563 Fragments arranged by size: 1027 563 99 BstEII G'GTnAC_C Cuts at: 0 43 1689 Size: 43 1646 BstXI CCAn_nnnn'nTGG Cuts at: 0 1110 1285 1689 Size: 1110 175 404 Fragments arranged by size: 1110 404 175 BtgI C'CryG_G Cuts at: 0 135 768 825 1047 1689 Size: 135 633 57 222 642 Fragments arranged by size: 642 633 222 135 57 BtsI GCAGTG_nn' Cuts at: 0 882 1682 1689 Size: 882 800 7 Fragments arranged by size: 882 800 7 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 858 893 1689 Size: 858 35 796 Fragments arranged by size: 858 796 35 DraIII CAC_nnn'GTG Cuts at: 0 1662 1689 Size: 1662 27 DrdI GACnn_nn'nnGTC Cuts at: 0 957 1689 Size: 957 732 EaeI y'GGCC_r Cuts at: 0 138 203 1689 Size: 138 65 1486 Fragments arranged by size: 1486 138 65 EarI CTCTTCn'nnn_ Cuts at: 0 40 785 1047 1109 1689 Size: 40 745 262 62 580 Fragments arranged by size: 745 580 262 62 40 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 924 1162 1327 1600 1689 Size: 924 238 165 273 89 Fragments arranged by size: 924 273 238 165 89 EcoO109I rG'GnC_Cy Cuts at: 0 83 295 1689 Size: 83 212 1394 Fragments arranged by size: 1394 212 83 FspI TGC'GCA Cuts at: 0 730 1689 Size: 730 959 HaeII r_GCGC'y Cuts at: 0 1323 1689 Size: 1323 366 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 345 377 1248 1280 1689 Size: 345 32 871 32 409 Fragments arranged by size: 871 409 345 32 32 HindIII A'AGCT_T Cuts at: 0 1342 1689 Size: 1342 347 KpnI G_GTAC'C Cuts at: 0 510 1689 Size: 510 1179 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 926 1162 1689 Size: 926 236 527 Fragments arranged by size: 926 527 236 MscI TGG'CCA Cuts at: 0 140 205 1689 Size: 140 65 1484 Fragments arranged by size: 1484 140 65 MslI CAynn'nnrTG Cuts at: 0 941 1225 1570 1689 Size: 941 284 345 119 Fragments arranged by size: 941 345 284 119 MspA1I CmG'CkG Cuts at: 0 18 1176 1211 1305 1689 Size: 18 1158 35 94 384 Fragments arranged by size: 1158 384 94 35 18 NdeI CA'TA_TG Cuts at: 0 849 1689 Size: 849 840 NsiI A_TGCA'T Cuts at: 0 1232 1689 Size: 1232 457 NspI r_CATG'y Cuts at: 0 1575 1689 Size: 1575 114 PasI CC'CwG_GG Cuts at: 0 785 1689 Size: 785 904 PflMI CCAn_nnn'nTGG Cuts at: 0 177 785 1689 Size: 177 608 904 Fragments arranged by size: 904 608 177 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1477 1509 1689 Size: 1477 32 180 Fragments arranged by size: 1477 180 32 PpuMI rG'GwC_Cy Cuts at: 0 295 1689 Size: 295 1394 PstI C_TGCA'G Cuts at: 0 1038 1469 1689 Size: 1038 431 220 Fragments arranged by size: 1038 431 220 PvuII CAG'CTG Cuts at: 0 1176 1211 1305 1689 Size: 1176 35 94 384 Fragments arranged by size: 1176 384 94 35 SapI GCTCTTCn'nnn_ Cuts at: 0 1109 1689 Size: 1109 580 SexAI A'CCwGG_T Cuts at: 0 596 1487 1689 Size: 596 891 202 Fragments arranged by size: 891 596 202 SfcI C'TryA_G Cuts at: 0 714 1034 1171 1465 1689 Size: 714 320 137 294 224 Fragments arranged by size: 714 320 294 224 137 SmlI C'TyrA_G Cuts at: 0 163 706 1689 Size: 163 543 983 Fragments arranged by size: 983 543 163 SphI G_CATG'C Cuts at: 0 1575 1689 Size: 1575 114 StuI AGG'CCT Cuts at: 0 129 1689 Size: 129 1560 StyI C'CwwG_G Cuts at: 0 449 869 953 1550 1689 Size: 449 420 84 597 139 Fragments arranged by size: 597 449 420 139 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 270 274 442 653 1423 1689 Size: 270 4 168 211 770 266 Fragments arranged by size: 770 270 266 211 168 4 TatI w'GTAC_w Cuts at: 0 629 1689 Size: 629 1060 Tth111I GACn'n_nGTC Cuts at: 0 1351 1689 Size: 1351 338 XmnI GAAnn'nnTTC Cuts at: 0 813 1689 Size: 813 876 Enzymes that do cut: Acc65I AcuI AflIII AleI AlfI AloI AlwNI AvaI BanI BanII BbvCI BciVI BclI BfrBI BglI BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsmI BsmBI Bsp1286I BsrBI BssSI BstEII BstXI BtgI BtsI CspCI DraIII DrdI EaeI EarI Eco57MI EcoO109I FspI HaeII Hin4I HindIII KpnI MmeI MscI MslI MspA1I NdeI NsiI NspI PasI PflMI PpiI PpuMI PstI PvuII SapI SexAI SfcI SmlI SphI StuI StyI TaqII TatI Tth111I XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AgeI AhdI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BcgI BglII BlpI Bme1580I BmtI BsaAI BsgI BsiHKAI BsiWI BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstYI BstZ17I Bsu36I BtgZI ClaI DraI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI HincII HpaI KasI MfeI MluI NaeI NarI NcoI NgoMIV NheI NotI NruI PacI PciI PfoI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TstI XbaI XcmI XhoI XmaI ZraI