Restriction map for
pFN21AE5687
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# NO CUT ENZYMES ( 125 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI AspEI AviII AvrII
BamHI BbeI BbrPI BbvCI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BspHI BspLU11I BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KspI MaeI
MaeII MfeI MflI MluI MroI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI XbaI XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 1553 GGTACC G^GTACC
AccII 1131 CGCG CG^CG
AcyI 1054 GACGCC G[GA]^CG[CT]C
AocI 1159 CCTGAGG CC^T[ACGT]AGG
Asp718I 1553 GGTACC G^GTACC
AspI 1257 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1590 TGGCCA TGG^CCA
BanII 429 GAGCCC G[GA]GC[CT]^C
BbsI 765 GAAGAC GAAGAC(2/6)
BcgI 603 GCACGCTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 765 GAAGAC GAAGAC(2/6)
BsaBI 1182 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1054 GACGCC G[GA]^CG[CT]C
BsaI 1321 GAGACC GGTCTC(1/5)
BsaWI 800 ACCGGA [AT]^CCGG[AT]
Bsp1407I 1613 TGTACA T^GTACA
BsrGI 1613 TGTACA T^GTACA
BstAPI 1149 GCACCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1131 CGCG CG^CG
BstXI 1029 CCACATCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1159 CCTGAGG CC^T[ACGT]AGG
CfoI 973 GCGC GCG^C
DrdI 1429 GACTACTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 15 CCACGG C^C[GA][CT]GG
EaeI 1590 TGGCCA [CT]^GGCC[GA]
Eco81I 1159 CCTGAGG CC^T[ACGT]AGG
EcoNI 1383 CCTCCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 972 GGCGCT [GA]GCGC^[CT]
HgaI 1054 GACGC GACGC(5/10)
HhaI 973 GCGC GCG^C
Hin1I 1054 GACGCC G[GA]^CG[CT]C
HinP1I 973 GCGC G^CGC
KpnI 1553 GGTACC GGTAC^C
MamI 1182 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1590 TGGCCA TGG^CCA
MscI 1590 TGGCCA TGG^CCA
MseI 692 TTAA T^TAA
MvnI 1131 CGCG CG^CG
PflFI 1257 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 774 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SexAI 1605 ACCTGGT A^CC[AT]GGT
SfcI 732 CTGTAG C^T[GA][CT]AG
SmlI 849 CTTGAG C^T[CT][GA]AG
SspBI 1613 TGTACA T^GTACA
TfiI 1673 GATTC G^A[AT]TC
Tru9I 692 TTAA T^TAA
Tsp509I 1198 AATT ^AATT
Tth111I 1257 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 774 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AlwNI 738 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
775 CAGCAGCTG
Asp700I 38 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1630 GAAGTTCTTC
AvaI 245 CCCGGG C^[CT]CG[GA]G
1097 CCCGGG
BsmI 21 GAATGC GAATGC(1/-1)
892 GAATGC
BsoBI 245 CCCGGG C^[CT]CG[GA]G
1097 CCCGGG
BsrBI 484 CCGCTC CCGCTC(-3/-3)
1265 CCGCTC
EarI 538 CTCTTC CTCTTC(1/4)
642 GAAGAG
EcoT14I 997 CCTTGG C^C[AT][AT]GG
1459 CCAAGG
EcoT22I 23 ATGCAT ATGCA^T
1294 ATGCAT
Ksp632I 538 CTCTTC CTCTTC(1/4)
642 GAAGAG
NsiI 23 ATGCAT ATGCA^T
1294 ATGCAT
SmaI 245 CCCGGG CCC^GGG
1097 CCCGGG
StyI 997 CCTTGG C^C[AT][AT]GG
1459 CCAAGG
TaqI 788 TCGA T^CGA
1409 TCGA
TthHB8I 788 TCGA T^CGA
1409 TCGA
XmaCI 245 CCCGGG C^CCGGG
1097 CCCGGG
XmaI 245 CCCGGG C^CCGGG
1097 CCCGGG
XmnI 38 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1630 GAAGTTCTTC
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# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BanI BbvI BcnI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI TseI Tsp45I
TspRI