Restriction map for
pFN21AE5687
(Circular) (Six-base) MAPSORT of: pFN21AE5687.seq Check: 6234 from: 1 to: 6391
pFN21AE5687 6391 bp
With 182 enzymes: SgfI *
December 8, 2014 13:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3557 3557
Size: 6391
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4201 278
Size: 53 83 186 936 2665 2468
Fragments arranged by size:
2665 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3734 1937
Size: 1797 4594
Acc65I G'GTAC_C
Cuts at: 3558 3712 3558
Size: 154 6237
AclI AA'CG_TT
Cuts at: 1563 4518 4891 1563
Size: 2955 373 3063
Fragments arranged by size:
3063 2955 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2034 2666 4454 5838 6106 1632
Size: 402 632 1788 1384 268 1917
Fragments arranged by size:
1917 1788 1384 632 402 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6194
AflIII A'CryG_T
Cuts at: 1203 5290 6267 1203
Size: 4087 977 1327
Fragments arranged by size:
4087 1327 977
AgeI A'CCGG_T
Cuts at: 5222 5222
Size: 6391
AhdI GACnn_n'nnGTC
Cuts at: 5120 5120
Size: 6391
AleI CACnn'nnGTG
Cuts at: 1166 3039 3626 1166
Size: 1873 587 3931
Fragments arranged by size:
3931 1873 587
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6391
AlwNI CAG_nnn'CTG
Cuts at: 2748 2785 4134 5706 2748
Size: 37 1349 1572 3433
Fragments arranged by size:
3433 1572 1349 37
ApaLI G'TGCA_C
Cuts at: 4448 5604 4448
Size: 1156 5235
ApoI r'AATT_y
Cuts at: 1493 3700 3926 4078 6187 1493
Size: 2207 226 152 2109 1697
Fragments arranged by size:
2207 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4945 160
Size: 872 3913 1606
Fragments arranged by size:
3913 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6391
AvaI C'yCGr_G
Cuts at: 1870 1943 2250 3102 3716 1870
Size: 73 307 852 614 4545
Fragments arranged by size:
4545 852 614 307 73
BamHI G'GATC_C
Cuts at: 3721 4088 3721
Size: 367 6024
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3026 3140 3558 3712 5167
Size: 325 217 627 1239 114 418 154 1455
Cuts at: 5167 618
Size: 1842
Fragments arranged by size:
1842 1455 1239 627 418 325 217 154
114
BanII G_rGCy'C
Cuts at: 1391 2438 3710 1391
Size: 1047 1272 4072
Fragments arranged by size:
4072 1272 1047
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2777 928
Size: 971 878 4542
Fragments arranged by size:
4542 971 878
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2597 2631 4582 4616 1903
Size: 34 660 34 1951 34 3678
Fragments arranged by size:
3678 1951 660 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4283 5493 4283
Size: 1210 5181
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6391
BfrBI ATG'CAT
Cuts at: 2030 3301 2030
Size: 1271 5120
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5002 136
Size: 107 122 71 631 605 3330 1525
Fragments arranged by size:
3330 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6386 6386
Size: 6391
BlpI GC'TnA_GC
Cuts at: 3808 3808
Size: 6391
Bme1580I G_kGCm'C
Cuts at: 1790 3143 3587 4452 5608 1790
Size: 1353 444 865 1156 2573
Fragments arranged by size:
2573 1353 1156 865 444
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6391
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2689 3058 4122
Size: 416 53 83 399 24 1272 369 1064
Cuts at: 4122 5080 442
Size: 958 1753
Fragments arranged by size:
1753 1272 1064 958 416 399 369 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6391
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3068 3190 3068
Size: 122 6269
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2019 2049 2064 2076 2079 2094 2109 2124
Size: 780 30 15 12 3 15 15 15
Cuts at: 2124 2154 2169 2184 2199 2214 2229 2909 3137
Size: 30 15 15 15 15 15 680 228
Cuts at: 3137 5051 1239
Size: 1914 2579
Fragments arranged by size:
2579 1914 780 680 228 30 30 15
15 15 15 15 15 15 15 15
12 3
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2875 4516 5381 5679 2875
Size: 1641 865 298 3587
Fragments arranged by size:
3587 1641 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3320 5054 882
Size: 617 1821 1734 2219
Fragments arranged by size:
2219 1821 1734 617
BsaAI yAC'GTr
Cuts at: 493 1204 4182 493
Size: 711 2978 2702
Fragments arranged by size:
2978 2702 711
BsaBI GATnn'nnATC
Cuts at: 3191 6385 3191
Size: 3194 3197
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3060 4198 4580 5215
Size: 53 83 186 936 1527 1138 382 635
Cuts at: 5215 275
Size: 1451
Fragments arranged by size:
1527 1451 1138 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2805 4824 5222 5496 5643 6054 2805
Size: 2019 398 274 147 411 3142
Fragments arranged by size:
3142 2019 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6166
Fragments arranged by size:
6166 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2498 2501 3230 1186
Size: 1312 3 729 4347
Fragments arranged by size:
4347 1312 729 3
BseYI C'CCAG_C
Cuts at: 2084 2144 2659 2785 2907 3479 3579 5594 6090
Size: 60 515 126 122 572 100 2015 496
Cuts at: 6090 2084
Size: 2385
Fragments arranged by size:
2385 2015 572 515 496 126 122 100
60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3002 3137 3419 1163
Size: 573 1266 135 282 4135
Fragments arranged by size:
4135 1266 573 282 135
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3841 4602 5630 1540
Size: 275 186 1840 761 1028 2301
Fragments arranged by size:
2301 1840 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2508 2803 3075 4452 4537 5608 2508
Size: 295 272 1377 85 1071 3291
Fragments arranged by size:
3291 1377 1071 295 272 85
BsmI GAATG_Cn'
Cuts at: 2032 2903 3902 3995 2032
Size: 871 999 93 4428
Fragments arranged by size:
4428 999 871 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2438 2508 2803 3075 3143 3587 3710
Size: 399 648 70 295 272 68 444 123
Cuts at: 3710 4452 4537 5608 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 648 444 399 295 272
123 85 70 68
BspEI T'CCGG_A
Cuts at: 6054 6054
Size: 6391
BspHI T'CATG_A
Cuts at: 4280 6010 4280
Size: 1730 4661
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2286 2783 3159 3338 3391 3557 3746 844
Size: 1442 497 376 179 53 166 189 3489
Fragments arranged by size:
3489 1442 497 376 189 179 166 53
BsrBI CCG'CTC
Cuts at: 1394 1823 2491 3272 4278 1394
Size: 429 668 781 1006 3507
Fragments arranged by size:
3507 1006 781 668 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2514 3177 3436 4886 5060 65
Size: 1183 1266 663 259 1450 174 1396
Fragments arranged by size:
1450 1396 1266 1183 663 259 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5035 5222 1700
Size: 110 3225 187 2869
Fragments arranged by size:
3225 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3618 96
Size: 3522 2869
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6391
BssSI C'ACGA_G
Cuts at: 4451 5463 4451
Size: 1012 5379
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3160 794
Size: 2366 4025
BstBI TT'CG_AA
Cuts at: 5195 5195
Size: 6391
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6391
BstXI CCAn_nnnn'nTGG
Cuts at: 3041 3041
Size: 6391
BstYI r'GATC_y
Cuts at: 1972 3721 4088 4473 4490 5942 6028 6040 6386
Size: 1749 367 385 17 1452 86 12 346
Cuts at: 6386 1972
Size: 1977
Fragments arranged by size:
1977 1749 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3165 3165
Size: 6391
BtgI C'CryG_G
Cuts at: 513 1065 2004 2020 513
Size: 552 939 16 4884
Fragments arranged by size:
4884 939 552 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3217 487
Size: 683 56 1991 3661
Fragments arranged by size:
3661 1991 683 56
BtsI GCAGTG_nn'
Cuts at: 2935 3075 3085 3907 4701 4721 2935
Size: 140 10 822 794 20 4605
Fragments arranged by size:
4605 822 794 140 20 10
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6391
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2349 2384 3269 3304 529
Size: 35 1785 35 885 35 3616
Fragments arranged by size:
3616 1785 885 35 35 35
DraI TTT'AAA
Cuts at: 3695 3695
Size: 6391
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3440 5398 809
Size: 507 2124 1958 1802
Fragments arranged by size:
2124 1958 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3595 3838 4727
Size: 54 1425 50 275 18 1765 243 889
Cuts at: 4727 6356 8
Size: 1629 43
Fragments arranged by size:
1765 1629 1425 889 275 243 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3838 1537
Size: 275 2026 4090
Fragments arranged by size:
4090 2026 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2549 2642 4321 1353
Size: 1196 93 1679 3423
Fragments arranged by size:
3423 1679 1196 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2296 2977 4978 5352 5498 387
Size: 1064 138 707 681 2001 374 146 1280
Fragments arranged by size:
2001 1280 1064 707 681 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2019 2034 2049 2064 2076 2079 2094
Size: 393 387 15 15 15 12 3 15
Cuts at: 2094 2109 2124 2154 2169 2184 2199 2214 2229
Size: 15 15 30 15 15 15 15 15
Cuts at: 2229 2666 2909 3137 4454 5051 5838 6106 1239
Size: 437 243 228 1317 597 787 268 1524
Fragments arranged by size:
1524 1317 787 597 437 393 387 268
243 228 30 15 15 15 15 15
15 15 15 15 15 15 12 3
EcoNI CCTnn'n_nnAGG
Cuts at: 3392 3392
Size: 6391
EcoO109I rG'GnC_Cy
Cuts at: 2283 2712 3112 3484 3527 2283
Size: 429 400 372 43 5147
Fragments arranged by size:
5147 429 400 372 43
EcoRI G'AATT_C
Cuts at: 3700 4078 3700
Size: 378 6013
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2064 2064
Size: 6391
FspI TGC'GCA
Cuts at: 4897 4897
Size: 6391
HaeII r_GCGC'y
Cuts at: 1678 2981 5538 1678
Size: 1303 2557 2531
Fragments arranged by size:
2557 2531 1303
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2747 2779 3362 3394 5032
Size: 32 135 32 1175 32 583 32 1638
Cuts at: 5032 5064 5106 5138 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1638 1175 583 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3735 3981 669
Size: 988 281 1797 246 3079
Fragments arranged by size:
3079 1797 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6391
HpaI GTT'AAC
Cuts at: 3981 3981
Size: 6391
KpnI G_GTAC'C
Cuts at: 3562 3716 3562
Size: 154 6237
MfeI C'AATT_G
Cuts at: 3990 3990
Size: 6391
MluI A'CGCG_T
Cuts at: 6267 6267
Size: 6391
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5505 5689 1305
Size: 4200 184 2007
Fragments arranged by size:
4200 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3597 10
Size: 54 1425 343 1765 2804
Fragments arranged by size:
2804 1765 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2025 3039 3626 4349 4708 4867 518
Size: 648 859 1014 587 723 359 159 2042
Fragments arranged by size:
2042 1014 859 723 648 587 359 159
MspA1I CmG'CkG
Cuts at: 1606 2054 2084 2144 2189 2785 2911 3522 3555
Size: 448 30 60 45 596 126 611 33
Cuts at: 3555 3579 3807 4484 5632 5877 1606
Size: 24 228 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 611 596 448 245 228
126 60 45 33 30 24
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4900
Fragments arranged by size:
4900 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6391
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6391
NotI GC'GGCC_GC
Cuts at: 3838 3838
Size: 6391
NsiI A_TGCA'T
Cuts at: 2032 3303 2032
Size: 1271 5120
NspI r_CATG'y
Cuts at: 1132 1229 3749 5294 1132
Size: 97 2520 1545 2229
Fragments arranged by size:
2520 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6391
PciI A'CATG_T
Cuts at: 5290 5290
Size: 6391
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2785 1108
Size: 1677 4714
PfoI T'CCnGG_A
Cuts at: 2874 3114 3390 2874
Size: 240 276 5875
Fragments arranged by size:
5875 276 240
PmeI GTTT'AAAC
Cuts at: 3695 3695
Size: 6391
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4460 4492 5973 6005 4460
Size: 32 1481 32 4846
Fragments arranged by size:
4846 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2283 2712 3112 3484 2283
Size: 429 400 372 5190
Fragments arranged by size:
5190 429 400 372
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6391
PsiI TTA'TAA
Cuts at: 3961 3961
Size: 6391
PstI C_TGCA'G
Cuts at: 830 3743 830
Size: 2913 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6391
PvuII CAG'CTG
Cuts at: 2054 2084 2144 2189 2785 3579 2054
Size: 30 60 45 596 794 4866
Fragments arranged by size:
4866 794 596 60 45 30
SalI G'TCGA_C
Cuts at: 1936 3733 1936
Size: 1797 4594
SbfI CC_TGCA'GG
Cuts at: 3743 3743
Size: 6391
ScaI AGT'ACT
Cuts at: 4639 6351 4639
Size: 1712 4679
SexAI A'CCwGG_T
Cuts at: 3610 3610
Size: 6391
SfcI C'TryA_G
Cuts at: 826 1045 2737 3739 4874 5555 5746 826
Size: 219 1692 1002 1135 681 191 1471
Fragments arranged by size:
1692 1471 1135 1002 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6391
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6391
SmaI CCC'GGG
Cuts at: 1872 2252 3104 3718 1872
Size: 380 852 614 4545
Fragments arranged by size:
4545 852 614 380
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2854 4495 5396 5658 820
Size: 197 926 911 1641 901 262 1553
Fragments arranged by size:
1641 1553 926 911 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4182 493
Size: 3689 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6391
SphI G_CATG'C
Cuts at: 1132 3749 1132
Size: 2617 3774
SrfI GCCC'GGGC
Cuts at: 2252 2252
Size: 6391
SspI AAT'ATT
Cuts at: 5 52 4315 5
Size: 47 4263 2081
Fragments arranged by size:
4263 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6391
StyI C'CwwG_G
Cuts at: 513 1065 2004 3002 3464 3830 513
Size: 552 939 998 462 366 3074
Fragments arranged by size:
3074 998 939 552 462 366
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3047 3058 4418 4435 4588 4773 5218
Size: 1255 1467 11 1360 17 153 185 445
Cuts at: 5218 325
Size: 1498
Fragments arranged by size:
1498 1467 1360 1255 445 185 153 17
11
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3618 4637 6349
Size: 274 80 33 51 1443 1641 1019 1712
Cuts at: 6349 96
Size: 138
Fragments arranged by size:
1712 1641 1443 1019 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2981 3013 3460 3492 6351 6383 2981
Size: 32 447 32 2859 32 2989
Fragments arranged by size:
2989 2859 447 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3265 1316
Size: 1949 4442
XbaI T'CTAG_A
Cuts at: 3727 3727
Size: 6391
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6391
XmaI C'CCGG_G
Cuts at: 1870 2250 3102 3716 1870
Size: 380 852 614 4545
Fragments arranged by size:
4545 852 614 380
XmnI GAAnn'nnTTC
Cuts at: 2047 3639 4520 2047
Size: 1592 881 3918
Fragments arranged by size:
3918 1592 881
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4199 276
Size: 53 83 186 936 2665 2468
Fragments arranged by size:
2665 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SfiI SfoI SwaI XcmI