Restriction map for
pFN21AE5687
(Linear) (Six-base) MAPSORT of: KIEE5687.seq Check: 5273 from: 1 to: 1686
KIEE5687.orf
With 182 enzymes: SgfI *
December 5, 2014 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1552 1686
Size: 1552 134
Acc65I G'GTAC_C
Cuts at: 0 1553 1686
Size: 1553 133
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 661 1686
Size: 29 632 1025
Fragments arranged by size:
1025 632 29
AleI CACnn'nnGTG
Cuts at: 0 1034 1621 1686
Size: 1034 587 65
Fragments arranged by size:
1034 587 65
AlwNI CAG_nnn'CTG
Cuts at: 0 743 780 1686
Size: 743 37 906
Fragments arranged by size:
906 743 37
AvaI C'yCGr_G
Cuts at: 0 245 1097 1686
Size: 245 852 589
Fragments arranged by size:
852 589 245
BanI G'GyrC_C
Cuts at: 0 1021 1135 1553 1686
Size: 1021 114 418 133
Fragments arranged by size:
1021 418 133 114
BanII G_rGCy'C
Cuts at: 0 433 1686
Size: 433 1253
BbsI GAAGACnn'nnnn_
Cuts at: 0 772 1686
Size: 772 914
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 592 626 1686
Size: 592 34 1060
Fragments arranged by size:
1060 592 34
BfrBI ATG'CAT
Cuts at: 0 25 1296 1686
Size: 25 1271 390
Fragments arranged by size:
1271 390 25
Bme1580I G_kGCm'C
Cuts at: 0 1138 1582 1686
Size: 1138 444 104
Fragments arranged by size:
1138 444 104
BmrI ACTGGGnnnn_n'
Cuts at: 0 684 1053 1686
Size: 684 369 633
Fragments arranged by size:
684 633 369
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1063 1185 1686
Size: 1063 122 501
Fragments arranged by size:
1063 501 122
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 14 44 59 71 74 89 104 119
Size: 14 30 15 12 3 15 15 15
Cuts at: 119 149 164 179 194 209 224 904 1132
Size: 30 15 15 15 15 15 680 228
Cuts at: 1132 1686
Size: 554
Fragments arranged by size:
680 554 228 30 30 15 15 15
15 15 15 15 15 15 14 12
3
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 870 1686
Size: 870 816
BsaI GGTCTCn'nnnn_
Cuts at: 0 1315 1686
Size: 1315 371
BsaBI GATnn'nnATC
Cuts at: 0 1186 1686
Size: 1186 500
BsaHI Gr'CG_yC
Cuts at: 0 1055 1686
Size: 1055 631
BsaWI w'CCGG_w
Cuts at: 0 800 1686
Size: 800 886
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 493 496 1225 1686
Size: 493 3 729 461
Fragments arranged by size:
729 493 461 3
BseYI C'CCAG_C
Cuts at: 0 79 139 654 780 902 1474 1574 1686
Size: 79 60 515 126 122 572 100 112
Fragments arranged by size:
572 515 126 122 112 100 79 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 997 1132 1414 1686
Size: 997 135 282 272
Fragments arranged by size:
997 282 272 135
BsiHKAI G_wGCw'C
Cuts at: 0 503 798 1070 1686
Size: 503 295 272 616
Fragments arranged by size:
616 503 295 272
BsmI GAATG_Cn'
Cuts at: 0 27 898 1686
Size: 27 871 788
Fragments arranged by size:
871 788 27
Bsp1286I G_dGCh'C
Cuts at: 0 433 503 798 1070 1138 1582 1686
Size: 433 70 295 272 68 444 104
Fragments arranged by size:
444 433 295 272 104 70 68
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 281 778 1154 1333 1386 1552 1686
Size: 281 497 376 179 53 166 134
Fragments arranged by size:
497 376 281 179 166 134 53
BsrBI CCG'CTC
Cuts at: 0 486 1267 1686
Size: 486 781 419
Fragments arranged by size:
781 486 419
BsrDI GCAATG_nn'
Cuts at: 0 509 1172 1431 1686
Size: 509 663 259 255
Fragments arranged by size:
663 509 259 255
BsrGI T'GTAC_A
Cuts at: 0 1613 1686
Size: 1613 73
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1155 1686
Size: 1155 531
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1036 1686
Size: 1036 650
Bsu36I CC'TnA_GG
Cuts at: 0 1160 1686
Size: 1160 526
BtgI C'CryG_G
Cuts at: 0 15 1686
Size: 15 1671
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1212 1686
Size: 1212 474
BtsI GCAGTG_nn'
Cuts at: 0 930 1070 1080 1686
Size: 930 140 10 606
Fragments arranged by size:
930 606 140 10
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 344 379 1264 1299 1686
Size: 344 35 885 35 387
Fragments arranged by size:
885 387 344 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1435 1686
Size: 1435 251
EaeI y'GGCC_r
Cuts at: 0 1590 1686
Size: 1590 96
EarI CTCTTCn'nnn_
Cuts at: 0 544 637 1686
Size: 544 93 1049
Fragments arranged by size:
1049 544 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 291 972 1686
Size: 291 681 714
Fragments arranged by size:
714 681 291
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 14 29 44 59 71 74 89 104
Size: 14 15 15 15 12 3 15 15
Cuts at: 104 119 149 164 179 194 209 224 661
Size: 15 30 15 15 15 15 15 437
Cuts at: 661 904 1132 1686
Size: 243 228 554
Fragments arranged by size:
554 437 243 228 30 15 15 15
15 15 15 15 15 15 15 15
14 12 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1387 1686
Size: 1387 299
EcoO109I rG'GnC_Cy
Cuts at: 0 278 707 1107 1479 1522 1686
Size: 278 429 400 372 43 164
Fragments arranged by size:
429 400 372 278 164 43
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 59 1686
Size: 59 1627
HaeII r_GCGC'y
Cuts at: 0 976 1686
Size: 976 710
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 742 774 1357 1389 1686
Size: 742 32 583 32 297
Fragments arranged by size:
742 583 297 32 32
KpnI G_GTAC'C
Cuts at: 0 1557 1686
Size: 1557 129
MscI TGG'CCA
Cuts at: 0 1592 1686
Size: 1592 94
MslI CAynn'nnrTG
Cuts at: 0 20 1034 1621 1686
Size: 20 1014 587 65
Fragments arranged by size:
1014 587 65 20
MspA1I CmG'CkG
Cuts at: 0 49 79 139 184 780 906 1517 1550
Size: 49 30 60 45 596 126 611 33
Cuts at: 1550 1574 1686
Size: 24 112
Fragments arranged by size:
611 596 126 112 60 49 45 33
30 24
NsiI A_TGCA'T
Cuts at: 0 27 1298 1686
Size: 27 1271 388
Fragments arranged by size:
1271 388 27
PflMI CCAn_nnn'nTGG
Cuts at: 0 780 1686
Size: 780 906
PfoI T'CCnGG_A
Cuts at: 0 869 1109 1385 1686
Size: 869 240 276 301
Fragments arranged by size:
869 301 276 240
PpuMI rG'GwC_Cy
Cuts at: 0 278 707 1107 1479 1686
Size: 278 429 400 372 207
Fragments arranged by size:
429 400 372 278 207
PvuII CAG'CTG
Cuts at: 0 49 79 139 184 780 1574 1686
Size: 49 30 60 45 596 794 112
Fragments arranged by size:
794 596 112 60 49 45 30
SexAI A'CCwGG_T
Cuts at: 0 1605 1686
Size: 1605 81
SfcI C'TryA_G
Cuts at: 0 732 1686
Size: 732 954
SmaI CCC'GGG
Cuts at: 0 247 1099 1686
Size: 247 852 587
Fragments arranged by size:
852 587 247
SmlI C'TyrA_G
Cuts at: 0 849 1686
Size: 849 837
SrfI GCCC'GGGC
Cuts at: 0 247 1686
Size: 247 1439
StyI C'CwwG_G
Cuts at: 0 997 1459 1686
Size: 997 462 227
Fragments arranged by size:
997 462 227
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1042 1053 1686
Size: 1042 11 633
Fragments arranged by size:
1042 633 11
TatI w'GTAC_w
Cuts at: 0 1613 1686
Size: 1613 73
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 976 1008 1455 1487 1686
Size: 976 32 447 32 199
Fragments arranged by size:
976 447 199 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1260 1686
Size: 1260 426
XmaI C'CCGG_G
Cuts at: 0 245 1097 1686
Size: 245 852 589
Fragments arranged by size:
852 589 245
XmnI GAAnn'nnTTC
Cuts at: 0 42 1634 1686
Size: 42 1592 52
Fragments arranged by size:
1592 52 42
Enzymes that do cut:
AarI Acc65I AcuI AleI AlwNI AvaI BanI BanII
BbsI BcgI BfrBI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspMI BsrBI BsrDI BsrGI BstAPI BstXI
Bsu36I BtgI BtgZI BtsI CspCI DrdI EaeI EarI
EciI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KpnI
MscI MslI MspA1I NsiI PflMI PfoI PpuMI PvuII
SexAI SfcI SmaI SmlI SrfI StyI TaqII TatI
TstI Tth111I XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BciVI BclI BglI
BglII BlpI BmgBI BmtI Bpu10I BsaAI BsaXI BsiEI
BsiWI BsmBI BspEI BspHI BsrFI BssHII BssSI BstBI
BstEII BstYI BstZ17I ClaI DraI DraIII EagI EcoICRI
EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI
KasI MfeI MluI MmeI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SspI StuI SwaI XbaI XcmI XhoI ZraI