################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI Alw44I ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII BbsI BcgI BciVI BfrI BlpI Bpu1102I BpuAI BsaBI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco52I EcoO65I EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MluI MroI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI Tru9I Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 400 GTCTAC GT^[AC][GT]AC AccII 1101 CGCG CG^CG AcsI 19 GAATTC [GA]^AATT[CT] AocI 460 CCTCAGG CC^T[ACGT]AGG Aor51HI 1628 AGCGCT AGC^GCT ApoI 19 GAATTC [GA]^AATT[CT] AvaI 345 CCCGGG C^[CT]CG[GA]G AvrII 1383 CCTAGG C^CTAGG BanII 132 GAGCCC G[GA]GC[CT]^C BbeI 442 GGCGCC GGCGC^C BbrPI 391 CACGTG CAC^GTG BclI 233 TGATCA T^GATCA BfaI 1384 CTAG C^TAG BglII 1275 AGATCT A^GATCT BlnI 1383 CCTAGG C^CTAGG BsaAI 391 CACGTG [CT]AC^GT[GA] BseRI 523 GAGGAG GAGGAG(10/8) BsoBI 345 CCCGGG C^[CT]CG[GA]G BspMI 1268 ACCTGC ACCTGC(4/8) BsrDI 1665 CATTGC GCAATG(2/0) BsrFI 386 GCCGGC [GA]^CCGG[CT] BstAPI 802 GCAGAGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 1101 CGCG CG^CG Bsu36I 460 CCTCAGG CC^T[ACGT]AGG Cfr10I 386 GCCGGC [GA]^CCGG[CT] Eco47III 1628 AGCGCT AGC^GCT Eco81I 460 CCTCAGG CC^T[ACGT]AGG EcoRI 19 GAATTC G^AATTC FauI 206 CCCGC CCCGC(4/6) FbaI 233 TGATCA T^GATCA KasI 442 GGCGCC G^GCGCC MaeI 1384 CTAG C^TAG MspA1I 1108 CAGCTG C[AC]G^C[GT]G MvnI 1101 CGCG CG^CG NaeI 386 GCCGGC GCC^GGC NarI 442 GGCGCC GG^CGCC NgoMIV 386 GCCGGC G^CCGGC PmaCI 391 CACGTG CAC^GTG PmlI 391 CACGTG CAC^GTG PpuMI 1249 GGGTCCC [GA]G^G[AT]CC[CT] PvuII 1108 CAGCTG CAG^CTG SfoI 442 GGCGCC GGC^GCC SmaI 345 CCCGGG CCC^GGG SphI 807 GCATGC GCATG^C TfiI 661 GAATC G^A[AT]TC Tsp45I 95 GTGAC ^GT[GC]AC Tsp509I 20 AATT ^AATT XmaCI 345 CCCGGG C^CCGGG XmaI 345 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AcyI 442 GGCGCC G[GA]^CG[CT]C 1430 GGCGTC AlwNI 463 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG 1033 CAGTTCCTG BamHI 341 GGATCC G^GATCC 488 GGATCC BglI 613 GCCTTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 701 GCCTCTATGGC BsaHI 442 GGCGCC G[GA]^CG[CT]C 1430 GGCGTC BsmBI 1432 CGTCTC CGTCTC(1/5) 1615 GAGACG DraII 713 AGGCCCT [GA]G^G[ACGT]CC[CT] 1249 GGGTCCC EcoNI 651 CCTGGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1404 CCTTGCTGAGG EcoO109I 713 AGGCCCT [GA]G^G[ACGT]CC[CT] 1249 GGGTCCC HgaI 413 GACGC GACGC(5/10) 1431 GCGTC Hin1I 442 GGCGCC G[GA]^CG[CT]C 1430 GGCGTC HphI 144 GGTGA GGTGA(8/7) 1497 TCACC MaeIII 83 GTAAC ^GT[ACGT]AC 95 GTGAC MslI 231 CATGATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 357 CACAGATGTG NspI 807 GCATGC [GA]CATG^[CT] 1469 GCATGT PleI 1216 GAGTC GAGTC(4/5) 1388 GAGTC SexAI 56 ACCTGGT A^CC[AT]GGT 986 ACCAGGT ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaII BalI BanI BbvCI BbvI BcnI BmyI BpmI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI Ksp632I MaeII MboI MboII MflI MluNI MnlI MscI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TseI TspRI TthHB8I XhoII