(Circular) (Six-base) MAPSORT of: pFN21AE6780.seq Check: 5761 from: 1 to: 6406 pFN21AE6780 6406 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4216 278 Size: 53 83 186 936 2680 2468 Fragments arranged by size: 2680 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2406 3749 1937 Size: 469 1343 4594 Fragments arranged by size: 4594 1343 469 Acc65I G'GTAC_C Cuts at: 3727 3727 Size: 6406 AclI AA'CG_TT Cuts at: 1563 4533 4906 1563 Size: 2970 373 3063 Fragments arranged by size: 3063 2970 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4469 5853 6121 1632 Size: 2837 1384 268 1917 Fragments arranged by size: 2837 1917 1384 268 AfeI AGC'GCT Cuts at: 3635 3635 Size: 6406 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6209 AflIII A'CryG_T Cuts at: 1203 5305 6282 1203 Size: 4102 977 1327 Fragments arranged by size: 4102 1327 977 AgeI A'CCGG_T Cuts at: 5237 5237 Size: 6406 AhdI GACnn_n'nnGTC Cuts at: 5135 5135 Size: 6406 AleI CACnn'nnGTG Cuts at: 1166 2366 1166 Size: 1200 5206 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2738 3677 1184 Size: 1554 939 3913 Fragments arranged by size: 3913 1554 939 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2315 2347 2468 2500 2315 Size: 32 121 32 6221 Fragments arranged by size: 6221 121 32 32 AlwNI CAG_nnn'CTG Cuts at: 2473 3043 4149 5721 2473 Size: 570 1106 1572 3158 Fragments arranged by size: 3158 1572 1106 570 ApaLI G'TGCA_C Cuts at: 4463 5619 4463 Size: 1156 5250 ApoI r'AATT_y Cuts at: 1493 2024 3715 3941 4093 6202 1493 Size: 531 1691 226 152 2109 1697 Fragments arranged by size: 2109 1697 1691 531 226 152 AseI AT'TA_AT Cuts at: 160 1032 4960 160 Size: 872 3928 1606 Fragments arranged by size: 3928 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6406 AvaI C'yCGr_G Cuts at: 1870 1943 2350 3731 1870 Size: 73 407 1381 4545 Fragments arranged by size: 4545 1381 407 73 AvrII C'CTAG_G Cuts at: 3388 3388 Size: 6406 BamHI G'GATC_C Cuts at: 2346 2493 3736 4103 2346 Size: 147 1243 367 4649 Fragments arranged by size: 4649 1243 367 147 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2447 2841 3354 3727 5182 Size: 325 217 627 660 394 513 373 1455 Cuts at: 5182 618 Size: 1842 Fragments arranged by size: 1842 1455 660 627 513 394 373 325 217 BanII G_rGCy'C Cuts at: 1391 2141 3725 1391 Size: 750 1584 4072 Fragments arranged by size: 4072 1584 750 BbeI G_GCGC'C Cuts at: 2451 2451 Size: 6406 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5435 BbvCI CC'TCA_GC Cuts at: 2517 2702 3414 2517 Size: 185 712 5509 Fragments arranged by size: 5509 712 185 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4597 4631 1903 Size: 34 2660 34 3678 Fragments arranged by size: 3678 2660 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4298 5508 4298 Size: 1210 5196 BclI T'GATC_A Cuts at: 1548 2238 1548 Size: 690 5716 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2624 2712 5017 Size: 107 122 71 631 605 952 88 2305 Cuts at: 5017 136 Size: 1525 Fragments arranged by size: 2305 1525 952 631 605 122 107 88 71 BglII A'GATC_T Cuts at: 3280 6401 3280 Size: 3121 3285 BlpI GC'TnA_GC Cuts at: 3823 3823 Size: 6406 Bme1580I G_kGCm'C Cuts at: 1790 2844 2867 2904 4467 5623 1790 Size: 1054 23 37 1563 1156 2573 Fragments arranged by size: 2573 1563 1156 1054 37 23 BmgBI CAC'GTC Cuts at: 1318 2200 3019 1318 Size: 882 819 4705 Fragments arranged by size: 4705 882 819 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2300 4137 5095 Size: 416 53 83 399 24 883 1837 958 Cuts at: 5095 442 Size: 1753 Fragments arranged by size: 1837 1753 958 883 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6406 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3439 3439 Size: 6406 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2495 3534 3583 5066 1239 Size: 1256 1039 49 1483 2579 Fragments arranged by size: 2579 1483 1256 1039 49 Bpu10I CC'TnA_GC Cuts at: 2517 2702 3414 2517 Size: 185 712 5509 Fragments arranged by size: 5509 712 185 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2054 2444 3595 4531 5396 5694 2054 Size: 390 1151 936 865 298 2766 Fragments arranged by size: 2766 1151 936 865 390 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2804 2888 3382 5069 882 Size: 617 1305 84 494 1687 2219 Fragments arranged by size: 2219 1687 1305 617 494 84 BsaAI yAC'GTr Cuts at: 493 1204 2398 4197 493 Size: 711 1194 1799 2702 Fragments arranged by size: 2702 1799 1194 711 BsaBI GATnn'nnATC Cuts at: 6400 6400 Size: 6406 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2448 3436 4213 4595 Size: 53 83 186 936 915 988 777 382 Cuts at: 4595 5230 275 Size: 635 1451 Fragments arranged by size: 1451 988 936 915 777 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4839 5237 5511 5658 6069 4839 Size: 398 274 147 411 5176 Fragments arranged by size: 5176 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2315 2345 2468 2492 2498 Size: 30 165 30 912 30 123 24 6 Cuts at: 2498 2522 2547 2577 3362 3392 1178 Size: 24 25 30 785 30 4192 Fragments arranged by size: 4192 912 785 165 123 30 30 30 30 30 25 24 24 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2543 1186 Size: 1357 5049 BseYI C'CCAG_C Cuts at: 3630 5609 6105 3630 Size: 1979 496 3931 Fragments arranged by size: 3931 1979 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2222 3119 3257 1163 Size: 573 486 897 138 4312 Fragments arranged by size: 4312 897 573 486 138 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3856 4617 5645 1540 Size: 275 186 1855 761 1028 2301 Fragments arranged by size: 2301 1855 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2070 2184 2312 3423 4467 4552 5623 2070 Size: 114 128 1111 1044 85 1071 2853 Fragments arranged by size: 2853 1111 1071 1044 128 114 85 BsmI GAATG_Cn' Cuts at: 3917 4010 3917 Size: 93 6313 BsmBI CGTCTCn'nnnn_ Cuts at: 3443 3614 3443 Size: 171 6235 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2070 2141 2184 2312 2844 2867 2904 Size: 399 280 71 43 128 532 23 37 Cuts at: 2904 3423 3725 4467 4552 5623 1391 Size: 519 302 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 532 519 399 302 280 128 85 71 43 37 23 BspEI T'CCGG_A Cuts at: 6069 6069 Size: 6406 BspHI T'CATG_A Cuts at: 4295 6025 4295 Size: 1730 4676 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3282 3761 844 Size: 2438 479 3489 Fragments arranged by size: 3489 2438 479 BsrBI CCG'CTC Cuts at: 1394 1823 4293 1394 Size: 429 2470 3507 Fragments arranged by size: 3507 2470 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3669 4901 5075 65 Size: 1183 2421 1232 174 1396 Fragments arranged by size: 2421 1396 1232 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2391 5050 5237 1700 Size: 110 581 2659 187 2869 Fragments arranged by size: 2869 2659 581 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6406 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6406 BssSI C'ACGA_G Cuts at: 4466 5478 4466 Size: 1012 5394 BstAPI GCAn_nnn'nTGC Cuts at: 794 2813 794 Size: 2019 4387 BstBI TT'CG_AA Cuts at: 5210 5210 Size: 6406 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6406 BstXI CCAn_nnnn'nTGG Cuts at: 3007 3505 3638 3007 Size: 498 133 5775 Fragments arranged by size: 5775 498 133 BstYI r'GATC_y Cuts at: 1972 2346 2470 2493 3280 3453 3468 3736 4103 Size: 374 124 23 787 173 15 268 367 Cuts at: 4103 4488 4505 5957 6043 6055 6401 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 787 385 374 367 346 268 173 124 86 23 17 15 12 Bsu36I CC'TnA_GG Cuts at: 2466 2466 Size: 6406 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4915 Fragments arranged by size: 4915 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5667 Fragments arranged by size: 5667 683 56 BtsI GCAGTG_nn' Cuts at: 2547 2902 2957 3922 4716 4736 2547 Size: 355 55 965 794 20 4217 Fragments arranged by size: 4217 965 794 355 55 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6406 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3214 3249 529 Size: 35 2650 35 3686 Fragments arranged by size: 3686 2650 35 35 DraI TTT'AAA Cuts at: 3710 3710 Size: 6406 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5413 809 Size: 507 4097 1802 Fragments arranged by size: 4097 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2016 2788 2997 Size: 54 1425 50 275 18 186 772 209 Cuts at: 2997 3327 3853 4742 6371 8 Size: 330 526 889 1629 43 Fragments arranged by size: 1629 1425 889 772 526 330 275 209 186 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3853 1537 Size: 275 2041 4090 Fragments arranged by size: 4090 2041 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2106 3290 3365 4336 1353 Size: 753 1184 75 971 3423 Fragments arranged by size: 3423 1184 971 753 75 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4993 5367 5513 387 Size: 1064 138 3404 374 146 1280 Fragments arranged by size: 3404 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2495 3534 3583 4469 5066 5853 6121 Size: 393 863 1039 49 886 597 787 268 Cuts at: 6121 1239 Size: 1524 Fragments arranged by size: 1524 1039 886 863 787 597 393 268 49 EcoNI CCTnn'n_nnAGG Cuts at: 2660 3413 2660 Size: 753 5653 EcoO109I rG'GnC_Cy Cuts at: 2719 3255 2719 Size: 536 5870 EcoRI G'AATT_C Cuts at: 2024 3715 4093 2024 Size: 1691 378 4337 Fragments arranged by size: 4337 1691 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2437 2635 2437 Size: 198 6208 FspI TGC'GCA Cuts at: 4912 4912 Size: 6406 HaeII r_GCGC'y Cuts at: 1678 2451 3513 3637 5553 1678 Size: 773 1062 124 1916 2531 Fragments arranged by size: 2531 1916 1062 773 124 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2230 2262 2492 2524 2651 Size: 32 135 32 658 32 230 32 127 Cuts at: 2651 2683 5047 5079 5121 5153 1373 Size: 32 2364 32 42 32 2626 Fragments arranged by size: 2626 2364 658 230 135 127 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3750 3996 669 Size: 988 281 1812 246 3079 Fragments arranged by size: 3079 1812 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6406 HpaI GTT'AAC Cuts at: 3996 3996 Size: 6406 KasI G'GCGC_C Cuts at: 2447 2447 Size: 6406 KpnI G_GTAC'C Cuts at: 3731 3731 Size: 6406 MfeI C'AATT_G Cuts at: 4005 4005 Size: 6406 MluI A'CGCG_T Cuts at: 6282 6282 Size: 6406 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5520 5704 1305 Size: 4215 184 2007 Fragments arranged by size: 4215 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2018 2790 2999 3329 10 Size: 54 1425 343 186 772 209 330 3087 Fragments arranged by size: 3087 1425 772 343 330 209 186 54 MslI CAynn'nnrTG Cuts at: 518 1166 2240 2366 4364 4723 4882 518 Size: 648 1074 126 1998 359 159 2042 Fragments arranged by size: 2042 1998 1074 648 359 159 126 MspA1I CmG'CkG Cuts at: 1606 3115 3822 4499 5647 5892 1606 Size: 1509 707 677 1148 245 2120 Fragments arranged by size: 2120 1509 1148 707 677 245 NaeI GCC'GGC Cuts at: 2393 2393 Size: 6406 NarI GG'CG_CC Cuts at: 2448 2448 Size: 6406 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4915 Fragments arranged by size: 4915 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6406 NgoMIV G'CCGG_C Cuts at: 2391 2391 Size: 6406 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6406 NotI GC'GGCC_GC Cuts at: 3853 3853 Size: 6406 NspI r_CATG'y Cuts at: 1132 1229 2816 3478 3764 5309 1132 Size: 97 1587 662 286 1545 2229 Fragments arranged by size: 2229 1587 1545 662 286 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6406 PciI A'CATG_T Cuts at: 5305 5305 Size: 6406 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6406 PfoI T'CCnGG_A Cuts at: 2028 2496 3126 3180 2028 Size: 468 630 54 5254 Fragments arranged by size: 5254 630 468 54 PmeI GTTT'AAAC Cuts at: 3710 3710 Size: 6406 PmlI CAC'GTG Cuts at: 2398 2398 Size: 6406 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2315 2347 2468 2500 4475 4507 5988 6020 2315 Size: 32 121 32 1975 32 1481 32 2701 Fragments arranged by size: 2701 1975 1481 121 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3255 3255 Size: 6406 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6406 PsiI TTA'TAA Cuts at: 3976 3976 Size: 6406 PstI C_TGCA'G Cuts at: 830 2055 2554 2952 3114 3758 830 Size: 1225 499 398 162 644 3478 Fragments arranged by size: 3478 1225 644 499 398 162 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6406 PvuII CAG'CTG Cuts at: 3115 3115 Size: 6406 SalI G'TCGA_C Cuts at: 1936 3748 1936 Size: 1812 4594 SanDI GG'GwC_CC Cuts at: 3255 3255 Size: 6406 SbfI CC_TGCA'GG Cuts at: 3758 3758 Size: 6406 ScaI AGT'ACT Cuts at: 4654 6366 4654 Size: 1712 4694 SexAI A'CCwGG_T Cuts at: 2061 2991 2061 Size: 930 5476 SfcI C'TryA_G Cuts at: 826 1045 2051 2372 2550 2948 3110 3531 3700 Size: 219 1006 321 178 398 162 421 169 Cuts at: 3700 3754 4889 5570 5761 826 Size: 54 1135 681 191 1471 Fragments arranged by size: 1471 1135 1006 681 421 398 321 219 191 178 169 162 54 SfoI GGC'GCC Cuts at: 2449 2449 Size: 6406 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6406 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6406 SmaI CCC'GGG Cuts at: 1872 2352 3733 1872 Size: 480 1381 4545 Fragments arranged by size: 4545 1381 480 SmlI C'TyrA_G Cuts at: 820 1017 1943 2069 2459 3610 4510 5411 5673 Size: 197 926 126 390 1151 900 901 262 Cuts at: 5673 820 Size: 1553 Fragments arranged by size: 1553 1151 926 901 900 390 262 197 126 SnaBI TAC'GTA Cuts at: 493 4197 493 Size: 3704 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6406 SphI G_CATG'C Cuts at: 1132 2816 3764 1132 Size: 1684 948 3774 Fragments arranged by size: 3774 1684 948 SspI AAT'ATT Cuts at: 5 52 4330 5 Size: 47 4278 2081 Fragments arranged by size: 4278 2081 47 StuI AGG'CCT Cuts at: 1517 2588 3060 3161 3698 1517 Size: 1071 472 101 537 4225 Fragments arranged by size: 4225 1071 537 472 101 StyI C'CwwG_G Cuts at: 513 1065 2004 2619 3000 3388 3693 3845 513 Size: 552 939 615 381 388 305 152 3074 Fragments arranged by size: 3074 939 615 552 388 381 305 152 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4433 4450 4603 4788 5233 325 Size: 1255 2853 17 153 185 445 1498 Fragments arranged by size: 2853 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2115 3027 4652 Size: 274 80 33 51 1443 138 912 1625 Cuts at: 4652 6364 96 Size: 1712 138 Fragments arranged by size: 1712 1625 1443 912 274 138 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2547 2579 6366 6398 2547 Size: 32 3787 32 2555 Fragments arranged by size: 3787 2555 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6406 XbaI T'CTAG_A Cuts at: 3742 3742 Size: 6406 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6406 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6406 XmaI C'CCGG_G Cuts at: 1870 2350 3731 1870 Size: 480 1381 4545 Fragments arranged by size: 4545 1381 480 XmnI GAAnn'nnTTC Cuts at: 4535 4535 Size: 6406 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4214 276 Size: 53 83 186 936 2680 2468 Fragments arranged by size: 2680 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI BaeI BfrBI BsiWI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SapI SfiI SrfI SwaI