(Linear) (Six-base) MAPSORT of: KIEE6780.seq Check: 118 from: 1 to: 1701 KIEE6780.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. AccI GT'mk_AC Cuts at: 0 401 1701 Size: 401 1300 AfeI AGC'GCT Cuts at: 0 1630 1701 Size: 1630 71 AleI CACnn'nnGTG Cuts at: 0 361 1701 Size: 361 1340 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 733 1672 1701 Size: 733 939 29 Fragments arranged by size: 939 733 29 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 310 342 463 495 1701 Size: 310 32 121 32 1206 Fragments arranged by size: 1206 310 121 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 468 1038 1701 Size: 468 570 663 Fragments arranged by size: 663 570 468 ApoI r'AATT_y Cuts at: 0 19 1701 Size: 19 1682 AvaI C'yCGr_G Cuts at: 0 345 1701 Size: 345 1356 AvrII C'CTAG_G Cuts at: 0 1383 1701 Size: 1383 318 BamHI G'GATC_C Cuts at: 0 341 488 1701 Size: 341 147 1213 Fragments arranged by size: 1213 341 147 BanI G'GyrC_C Cuts at: 0 442 836 1349 1701 Size: 442 394 513 352 Fragments arranged by size: 513 442 394 352 BanII G_rGCy'C Cuts at: 0 136 1701 Size: 136 1565 BbeI G_GCGC'C Cuts at: 0 446 1701 Size: 446 1255 BbvCI CC'TCA_GC Cuts at: 0 512 697 1409 1701 Size: 512 185 712 292 Fragments arranged by size: 712 512 292 185 BclI T'GATC_A Cuts at: 0 233 1701 Size: 233 1468 BglI GCCn_nnn'nGGC Cuts at: 0 619 707 1701 Size: 619 88 994 Fragments arranged by size: 994 619 88 BglII A'GATC_T Cuts at: 0 1275 1701 Size: 1275 426 Bme1580I G_kGCm'C Cuts at: 0 839 862 899 1701 Size: 839 23 37 802 Fragments arranged by size: 839 802 37 23 BmgBI CAC'GTC Cuts at: 0 195 1014 1701 Size: 195 819 687 Fragments arranged by size: 819 687 195 BmrI ACTGGGnnnn_n' Cuts at: 0 295 1701 Size: 295 1406 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1434 1701 Size: 1434 267 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 490 1529 1578 1701 Size: 490 1039 49 123 Fragments arranged by size: 1039 490 123 49 Bpu10I CC'TnA_GC Cuts at: 0 512 697 1409 1701 Size: 512 185 712 292 Fragments arranged by size: 712 512 292 185 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 49 439 1590 1701 Size: 49 390 1151 111 Fragments arranged by size: 1151 390 111 49 BsaI GGTCTCn'nnnn_ Cuts at: 0 799 883 1377 1701 Size: 799 84 494 324 Fragments arranged by size: 799 494 324 84 BsaAI yAC'GTr Cuts at: 0 393 1701 Size: 393 1308 BsaHI Gr'CG_yC Cuts at: 0 443 1431 1701 Size: 443 988 270 Fragments arranged by size: 988 443 270 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 310 340 463 487 493 517 542 572 Size: 310 30 123 24 6 24 25 30 Cuts at: 572 1357 1387 1701 Size: 785 30 314 Fragments arranged by size: 785 314 310 123 30 30 30 25 24 24 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 538 1701 Size: 538 1163 BseYI C'CCAG_C Cuts at: 0 1625 1701 Size: 1625 76 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 217 1114 1252 1701 Size: 217 897 138 449 Fragments arranged by size: 897 449 217 138 BsiHKAI G_wGCw'C Cuts at: 0 65 179 307 1418 1701 Size: 65 114 128 1111 283 Fragments arranged by size: 1111 283 128 114 65 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1438 1609 1701 Size: 1438 171 92 Fragments arranged by size: 1438 171 92 Bsp1286I G_dGCh'C Cuts at: 0 65 136 179 307 839 862 899 1418 Size: 65 71 43 128 532 23 37 519 Cuts at: 1418 1701 Size: 283 Fragments arranged by size: 532 519 283 128 71 65 43 37 23 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1277 1701 Size: 1277 424 BsrDI GCAATG_nn' Cuts at: 0 1664 1701 Size: 1664 37 BsrFI r'CCGG_y Cuts at: 0 386 1701 Size: 386 1315 BstAPI GCAn_nnn'nTGC Cuts at: 0 808 1701 Size: 808 893 BstXI CCAn_nnnn'nTGG Cuts at: 0 1002 1500 1633 1701 Size: 1002 498 133 68 Fragments arranged by size: 1002 498 133 68 BstYI r'GATC_y Cuts at: 0 341 465 488 1275 1448 1463 1701 Size: 341 124 23 787 173 15 238 Fragments arranged by size: 787 341 238 173 124 23 15 Bsu36I CC'TnA_GG Cuts at: 0 461 1701 Size: 461 1240 BtsI GCAGTG_nn' Cuts at: 0 542 897 952 1701 Size: 542 355 55 749 Fragments arranged by size: 749 542 355 55 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1209 1244 1701 Size: 1209 35 457 Fragments arranged by size: 1209 457 35 EaeI y'GGCC_r Cuts at: 0 11 783 992 1322 1701 Size: 11 772 209 330 379 Fragments arranged by size: 772 379 330 209 11 EarI CTCTTCn'nnn_ Cuts at: 0 101 1285 1360 1701 Size: 101 1184 75 341 Fragments arranged by size: 1184 341 101 75 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 490 1529 1578 1701 Size: 490 1039 49 123 Fragments arranged by size: 1039 490 123 49 EcoNI CCTnn'n_nnAGG Cuts at: 0 655 1408 1701 Size: 655 753 293 Fragments arranged by size: 753 655 293 EcoO109I rG'GnC_Cy Cuts at: 0 714 1250 1701 Size: 714 536 451 Fragments arranged by size: 714 536 451 EcoRI G'AATT_C Cuts at: 0 19 1701 Size: 19 1682 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 432 630 1701 Size: 432 198 1071 Fragments arranged by size: 1071 432 198 HaeII r_GCGC'y Cuts at: 0 446 1508 1632 1701 Size: 446 1062 124 69 Fragments arranged by size: 1062 446 124 69 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 225 257 487 519 646 678 1701 Size: 225 32 230 32 127 32 1023 Fragments arranged by size: 1023 230 225 127 32 32 32 KasI G'GCGC_C Cuts at: 0 442 1701 Size: 442 1259 MscI TGG'CCA Cuts at: 0 13 785 994 1324 1701 Size: 13 772 209 330 377 Fragments arranged by size: 772 377 330 209 13 MslI CAynn'nnrTG Cuts at: 0 235 361 1701 Size: 235 126 1340 Fragments arranged by size: 1340 235 126 MspA1I CmG'CkG Cuts at: 0 1110 1701 Size: 1110 591 NaeI GCC'GGC Cuts at: 0 388 1701 Size: 388 1313 NarI GG'CG_CC Cuts at: 0 443 1701 Size: 443 1258 NgoMIV G'CCGG_C Cuts at: 0 386 1701 Size: 386 1315 NspI r_CATG'y Cuts at: 0 811 1473 1701 Size: 811 662 228 Fragments arranged by size: 811 662 228 PfoI T'CCnGG_A Cuts at: 0 23 491 1121 1175 1701 Size: 23 468 630 54 526 Fragments arranged by size: 630 526 468 54 23 PmlI CAC'GTG Cuts at: 0 393 1701 Size: 393 1308 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 310 342 463 495 1701 Size: 310 32 121 32 1206 Fragments arranged by size: 1206 310 121 32 32 PpuMI rG'GwC_Cy Cuts at: 0 1250 1701 Size: 1250 451 PstI C_TGCA'G Cuts at: 0 50 549 947 1109 1701 Size: 50 499 398 162 592 Fragments arranged by size: 592 499 398 162 50 PvuII CAG'CTG Cuts at: 0 1110 1701 Size: 1110 591 SanDI GG'GwC_CC Cuts at: 0 1250 1701 Size: 1250 451 SexAI A'CCwGG_T Cuts at: 0 56 986 1701 Size: 56 930 715 Fragments arranged by size: 930 715 56 SfcI C'TryA_G Cuts at: 0 46 367 545 943 1105 1526 1695 1701 Size: 46 321 178 398 162 421 169 6 Fragments arranged by size: 421 398 321 178 169 162 46 6 SfoI GGC'GCC Cuts at: 0 444 1701 Size: 444 1257 SmaI CCC'GGG Cuts at: 0 347 1701 Size: 347 1354 SmlI C'TyrA_G Cuts at: 0 64 454 1605 1701 Size: 64 390 1151 96 Fragments arranged by size: 1151 390 96 64 SphI G_CATG'C Cuts at: 0 811 1701 Size: 811 890 StuI AGG'CCT Cuts at: 0 583 1055 1156 1693 1701 Size: 583 472 101 537 8 Fragments arranged by size: 583 537 472 101 8 StyI C'CwwG_G Cuts at: 0 614 995 1383 1688 1701 Size: 614 381 388 305 13 Fragments arranged by size: 614 388 381 305 13 TatI w'GTAC_w Cuts at: 0 110 1022 1701 Size: 110 912 679 Fragments arranged by size: 912 679 110 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 542 574 1701 Size: 542 32 1127 Fragments arranged by size: 1127 542 32 XmaI C'CCGG_G Cuts at: 0 345 1701 Size: 345 1356 Enzymes that do cut: AccI AfeI AleI AlfI AloI AlwNI ApoI AvaI AvrII BamHI BanI BanII BbeI BbvCI BclI BglI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI BstAPI BstXI BstYI Bsu36I BtsI CspCI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI NgoMIV NspI PfoI PmlI PpiI PpuMI PstI PvuII SanDI SexAI SfcI SfoI SmaI SmlI SphI StuI StyI TatI TstI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AflII AflIII AgeI AhdI ApaI ApaLI AscI AseI AsiSI BaeI BbsI BcgI BciVI BfrBI BlpI BmtI BsaBI BsaWI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgI BtgZI ClaI DraI DraIII DrdI EagI EciI EcoICRI EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI NcoI NdeI NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TaqII Tth111I XbaI XcmI XhoI XmnI ZraI