Restriction map for
pFN21AE5650
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccII AccIII AcsI
AgeI AhdI Alw44I AocI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI BbeI BbrPI
BglI BglII BlpI Bpu1102I BsaI
BsaWI BseAI BsgI BsiWI BsmAI
BsmBI BsmI BspDI BspEI BsrBI
BsrDI BssHII BstBI BstEII BstPI
BstUI BstXI Bsu36I CelII ClaI
CpoI DraI DrdI EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FseI
HincII HindII HpaI KasI KpnI
Ksp632I KspI MfeI MluI MroI
MunI MvnI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AclI 1042 AACGTT AA^CGTT
AcyI 1090 GACGCC G[GA]^CG[CT]C
AflII 1078 CTTAAG C^TTAAG
Aor51HI 266 AGCGCT AGC^GCT
Asp700I 991 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 760 CCCGAG C^[CT]CG[GA]G
AviII 239 TGCGCA TGC^GCA
AvrII 918 CCTAGG C^CTAGG
BalI 615 TGGCCA TGG^CCA
BamHI 1452 GGATCC G^GATCC
BanI 366 GGTGCC G^G[CT][GA]CC
BanII 819 GGGCTC G[GA]GC[CT]^C
BbvCI 1152 CCTCAGC CCTCAGC(-5/-2)
BcgI 882 CGAGGAGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 512 GTATCC GTATCC(6/5)
BclI 936 TGATCA T^GATCA
BcnI 423 CCGGG CC^[GC]GG
BfrI 1078 CTTAAG C^TTAAG
BlnI 918 CCTAGG C^CTAGG
BpmI 439 CTCCAG CTGGAG(16/14)
BsaAI 73 TACGTG [CT]AC^GT[GA]
BsaHI 1090 GACGCC G[GA]^CG[CT]C
BsiEI 129 CGACCG CG[GA][CT]^CG
BsoBI 760 CCCGAG C^[CT]CG[GA]G
Bsp1407I 551 TGTACA T^GTACA
BspHI 137 TCATGA T^CATGA
BsrFI 182 GCCGGC [GA]^CCGG[CT]
BsrGI 551 TGTACA T^GTACA
BssSI 1003 CACGAG CACGAG(-5/-1)
Bst1107I 525 GTATAC GTA^TAC
BstAPI 285 GCACCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 525 GTATAC GTA^TAC
Cfr10I 182 GCCGGC [GA]^CCGG[CT]
DraIII 286 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 121 CCGTGG C^C[GA][CT]GG
Eco47III 266 AGCGCT AGC^GCT
FauI 92 CCCGC CCCGC(4/6)
FbaI 936 TGATCA T^GATCA
FspI 239 TGCGCA TGC^GCA
Hin1I 1090 GACGCC G[GA]^CG[CT]C
MluNI 615 TGGCCA TGG^CCA
MscI 615 TGGCCA TGG^CCA
NaeI 182 GCCGGC GCC^GGC
NciI 423 CCGGG CC^[GC]GG
NgoMIV 182 GCCGGC G^CCGGC
PshAI 78 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1042 AACGTT AA^CGTT
PvuII 782 CAGCTG CAG^CTG
RcaI 137 TCATGA T^CATGA
SexAI 287 ACCTGGT A^CC[AT]GGT
SspBI 551 TGTACA T^GTACA
Tsp509I 1280 AATT ^AATT
XmnI 991 GAATCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 259 GTCTAC GT^[AC][GT]AC
525 GTATAC
AflIII 356 ACATGT A^C[GA][CT]GT
743 ACATGT
AspHI 229 GTGCTC G[AT]GC[AT]^C
1312 GAGCAC
BbsI 1057 GAAGAC GAAGAC(2/6)
643 GTCTTC
BpuAI 1057 GAAGAC GAAGAC(2/6)
643 GTCTTC
BsaBI 507 GATTAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
937 GATCACCATC
BsiHKAI 229 GTGCTC G[AT]GC[AT]^C
1312 GAGCAC
BspLU11I 356 ACATGT A^CATGT
743 ACATGT
BspMI 116 ACCTGC ACCTGC(4/8)
722 ACCTGC
BstYI 1376 AGATCC [GA]^GATC[CT]
1452 GGATCC
EaeI 615 TGGCCA [CT]^GGCC[GA]
909 TGGCCG
MamI 507 GATTAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
937 GATCACCATC
MflI 1376 AGATCC [GA]^GATC[CT]
1452 GGATCC
MslI 487 CACCTGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1161 CACTCCCATG
MspA1I 93 CCGCTG C[AC]G^C[GT]G
782 CAGCTG
NspI 356 ACATGT [GA]CATG^[CT]
743 ACATGT
PflMI 282 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
618 CCATGAGCTGG
PleI 15 GACTC GAGTC(4/5)
309 GACTC
PpuMI 425 GGGACCC [GA]G^G[AT]CC[CT]
719 AGGACCT
PstI 655 CTGCAG CTGCA^G
925 CTGCAG
TfiI 419 GAATC G^A[AT]TC
991 GAATC
Van91I 282 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
618 CCATGAGCTGG
XhoII 1376 AGATCC [GA]^GATC[CT]
1452 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BbvI BfaI BmyI BsaJI
BseRI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MnlI MseI MspI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI Tru9I
TseI Tsp45I TspRI TthHB8I