(Circular) (Six-base) MAPSORT of: pFN21AE5650.seq Check: 7264 from: 1 to: 6199 pFN21AE5650 6199 bp With 182 enzymes: SgfI * December 8, 2014 13:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4009 278 Size: 53 83 186 936 2473 2468 Fragments arranged by size: 2473 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2265 2531 3542 1937 Size: 328 266 1011 4594 Fragments arranged by size: 4594 1011 328 266 Acc65I G'GTAC_C Cuts at: 3520 3520 Size: 6199 AclI AA'CG_TT Cuts at: 1563 3048 4326 4699 1563 Size: 1485 1278 373 3063 Fragments arranged by size: 3063 1485 1278 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2708 4262 5646 5914 1632 Size: 1076 1554 1384 268 1917 Fragments arranged by size: 1917 1554 1384 1076 268 AfeI AGC'GCT Cuts at: 2273 2273 Size: 6199 AflII C'TTAA_G Cuts at: 820 1017 3083 820 Size: 197 2066 3936 Fragments arranged by size: 3936 2066 197 AflIII A'CryG_T Cuts at: 1203 2361 2748 5098 6075 1203 Size: 1158 387 2350 977 1327 Fragments arranged by size: 2350 1327 1158 977 387 AgeI A'CCGG_T Cuts at: 5030 5030 Size: 6199 AhdI GACnn_n'nnGTC Cuts at: 4928 4928 Size: 6199 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6199 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6199 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2849 2881 2849 Size: 32 6167 AlwNI CAG_nnn'CTG Cuts at: 2293 2452 2596 2728 3942 5514 2293 Size: 159 144 132 1214 1572 2978 Fragments arranged by size: 2978 1572 1214 159 144 132 ApaLI G'TGCA_C Cuts at: 4256 5412 4256 Size: 1156 5043 ApoI r'AATT_y Cuts at: 1493 3508 3734 3886 5995 1493 Size: 2015 226 152 2109 1697 Fragments arranged by size: 2109 2015 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4753 160 Size: 872 3721 1606 Fragments arranged by size: 3721 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6199 AvaI C'yCGr_G Cuts at: 1870 1943 2765 3524 1870 Size: 73 822 759 4545 Fragments arranged by size: 4545 822 759 73 AvrII C'CTAG_G Cuts at: 2923 2923 Size: 6199 BamHI G'GATC_C Cuts at: 3457 3529 3896 3457 Size: 72 367 5760 Fragments arranged by size: 5760 367 72 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2371 3520 4975 618 Size: 325 217 627 584 1149 1455 1842 Fragments arranged by size: 1842 1455 1149 627 584 325 217 BanII G_rGCy'C Cuts at: 1391 2828 3518 1391 Size: 1437 690 4072 Fragments arranged by size: 4072 1437 690 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2641 3069 928 Size: 971 742 428 4058 Fragments arranged by size: 4058 971 742 428 BbvCI CC'TCA_GC Cuts at: 3158 3158 Size: 6199 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2876 2910 4390 4424 1903 Size: 34 939 34 1480 34 3678 Fragments arranged by size: 3678 1480 939 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2528 4091 5301 2528 Size: 1563 1210 3426 Fragments arranged by size: 3426 1563 1210 BclI T'GATC_A Cuts at: 1548 2941 1548 Size: 1393 4806 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4810 136 Size: 107 122 71 631 605 3138 1525 Fragments arranged by size: 3138 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6194 6194 Size: 6199 BlpI GC'TnA_GC Cuts at: 3616 3616 Size: 6199 Bme1580I G_kGCm'C Cuts at: 1790 2376 2646 3145 4260 5416 1790 Size: 586 270 499 1115 1156 2573 Fragments arranged by size: 2573 1156 1115 586 499 270 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6199 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2794 3930 4888 Size: 416 53 83 399 24 1377 1136 958 Cuts at: 4888 442 Size: 1753 Fragments arranged by size: 1753 1377 1136 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6199 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2429 4859 1239 Size: 1190 2430 2579 Fragments arranged by size: 2579 2430 1190 Bpu10I CC'TnA_GC Cuts at: 3158 3158 Size: 6199 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2950 3318 4324 5189 5487 2950 Size: 368 1006 865 298 3662 Fragments arranged by size: 3662 1006 865 368 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4862 882 Size: 617 3363 2219 Fragments arranged by size: 3363 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2080 3990 493 Size: 711 876 1910 2702 Fragments arranged by size: 2702 1910 876 711 BsaBI GATnn'nnATC Cuts at: 2516 2946 6193 2516 Size: 430 3247 2522 Fragments arranged by size: 3247 2522 430 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3096 4006 4388 5023 Size: 53 83 186 936 1563 910 382 635 Cuts at: 5023 275 Size: 1451 Fragments arranged by size: 1563 1451 936 910 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4632 5030 5304 5451 5862 4632 Size: 398 274 147 411 4969 Fragments arranged by size: 4969 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2849 2879 1178 Size: 30 165 30 1446 30 4498 Fragments arranged by size: 4498 1446 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2870 2903 3355 1186 Size: 1684 33 452 4030 Fragments arranged by size: 4030 1684 452 33 BseYI C'CCAG_C Cuts at: 2068 2279 2286 2602 5402 5898 2068 Size: 211 7 316 2800 496 2369 Fragments arranged by size: 2800 2369 496 316 211 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5626 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2137 3649 4410 5438 1540 Size: 275 186 136 1512 761 1028 2301 Fragments arranged by size: 2301 1512 1028 761 275 186 136 BsiHKAI G_wGCw'C Cuts at: 2238 3321 4260 4345 5416 2238 Size: 1083 939 85 1071 3021 Fragments arranged by size: 3021 1083 1071 939 85 BsmI GAATG_Cn' Cuts at: 3710 3803 3710 Size: 93 6106 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2238 2376 2646 2828 3145 3321 3518 Size: 399 448 138 270 182 317 176 197 Cuts at: 3518 4260 4345 5416 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 448 399 317 270 197 182 176 138 85 BspEI T'CCGG_A Cuts at: 5862 5862 Size: 6199 BspHI T'CATG_A Cuts at: 2142 4088 5818 2142 Size: 1946 1730 2523 Fragments arranged by size: 2523 1946 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2130 2736 3554 844 Size: 1286 606 818 3489 Fragments arranged by size: 3489 1286 818 606 BsrBI CCG'CTC Cuts at: 1394 1823 4086 1394 Size: 429 2263 3507 Fragments arranged by size: 3507 2263 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4694 4868 65 Size: 1183 3446 174 1396 Fragments arranged by size: 3446 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2187 4843 5030 1700 Size: 110 377 2656 187 2869 Fragments arranged by size: 2869 2656 377 187 110 BsrGI T'GTAC_A Cuts at: 96 2556 96 Size: 2460 3739 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6199 BssSI C'ACGA_G Cuts at: 3008 4259 5271 3008 Size: 1251 1012 3936 Fragments arranged by size: 3936 1251 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2296 794 Size: 1502 4697 BstBI TT'CG_AA Cuts at: 5003 5003 Size: 6199 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6199 BstYI r'GATC_y Cuts at: 1972 3381 3457 3529 3896 4281 4298 5750 5836 Size: 1409 76 72 367 385 17 1452 86 Cuts at: 5836 5848 6194 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1409 385 367 346 86 76 72 17 12 BstZ17I GTA'TAC Cuts at: 2532 2532 Size: 6199 BtgI C'CryG_G Cuts at: 513 1065 2126 513 Size: 552 1061 4586 Fragments arranged by size: 4586 1061 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3315 487 Size: 683 56 2089 3371 Fragments arranged by size: 3371 2089 683 56 BtsI GCAGTG_nn' Cuts at: 3715 4509 4529 3715 Size: 794 20 5385 Fragments arranged by size: 5385 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6199 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6164 DraI TTT'AAA Cuts at: 3503 3503 Size: 6199 DraIII CAC_nnn'GTG Cuts at: 2296 2296 Size: 6199 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5206 809 Size: 507 3890 1802 Fragments arranged by size: 3890 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2620 2914 3646 Size: 54 1425 50 275 18 790 294 732 Cuts at: 3646 4535 6164 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 790 732 294 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3646 1537 Size: 275 1834 4090 Fragments arranged by size: 4090 1834 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4129 1353 Size: 2776 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2785 4786 5160 5306 387 Size: 1064 138 1196 2001 374 146 1280 Fragments arranged by size: 2001 1280 1196 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2429 2708 4262 4859 5646 5914 1239 Size: 393 797 279 1554 597 787 268 1524 Fragments arranged by size: 1554 1524 797 787 597 393 279 268 EcoO109I rG'GnC_Cy Cuts at: 2431 2725 3013 3306 2431 Size: 294 288 293 5324 Fragments arranged by size: 5324 294 293 288 EcoRI G'AATT_C Cuts at: 3508 3886 3508 Size: 378 5821 FspI TGC'GCA Cuts at: 2246 4705 2246 Size: 2459 3740 HaeII r_GCGC'y Cuts at: 1678 2275 2286 3200 5346 1678 Size: 597 11 914 2146 2531 Fragments arranged by size: 2531 2146 914 597 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2409 2441 4840 4872 4914 Size: 32 135 32 837 32 2399 32 42 Cuts at: 4914 4946 1373 Size: 32 2626 Fragments arranged by size: 2626 2399 837 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3543 3789 669 Size: 988 281 1605 246 3079 Fragments arranged by size: 3079 1605 988 281 246 HindIII A'AGCT_T Cuts at: 748 3080 3206 3244 748 Size: 2332 126 38 3703 Fragments arranged by size: 3703 2332 126 38 HpaI GTT'AAC Cuts at: 3789 3789 Size: 6199 KpnI G_GTAC'C Cuts at: 3524 3524 Size: 6199 MfeI C'AATT_G Cuts at: 3798 3798 Size: 6199 MluI A'CGCG_T Cuts at: 6075 6075 Size: 6199 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2316 5313 5497 1305 Size: 1011 2997 184 2007 Fragments arranged by size: 2997 2007 1011 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2622 10 Size: 54 1425 343 790 3587 Fragments arranged by size: 3587 1425 790 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2496 3170 4157 4516 4675 518 Size: 648 1330 674 987 359 159 2042 Fragments arranged by size: 2042 1330 987 674 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2100 2789 3615 4292 5440 5685 1606 Size: 494 689 826 677 1148 245 2120 Fragments arranged by size: 2120 1148 826 689 677 494 245 NaeI GCC'GGC Cuts at: 2189 2189 Size: 6199 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5647 NdeI CA'TA_TG Cuts at: 387 387 Size: 6199 NgoMIV G'CCGG_C Cuts at: 2187 2187 Size: 6199 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6199 NotI GC'GGCC_GC Cuts at: 3646 3646 Size: 6199 NspI r_CATG'y Cuts at: 1132 1229 2365 2752 3557 5102 1132 Size: 97 1136 387 805 1545 2229 Fragments arranged by size: 2229 1545 1136 805 387 97 PasI CC'CwG_GG Cuts at: 1348 2376 2955 1348 Size: 1028 579 4592 Fragments arranged by size: 4592 1028 579 PciI A'CATG_T Cuts at: 2361 2748 5098 2361 Size: 387 2350 3462 Fragments arranged by size: 3462 2350 387 PflMI CCAn_nnn'nTGG Cuts at: 1108 2293 2629 1108 Size: 1185 336 4678 Fragments arranged by size: 4678 1185 336 PfoI T'CCnGG_A Cuts at: 2427 2427 Size: 6199 PmeI GTTT'AAAC Cuts at: 3503 3503 Size: 6199 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2849 2881 4268 4300 5781 5813 2849 Size: 32 1387 32 1481 32 3235 Fragments arranged by size: 3235 1481 1387 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2431 2725 2431 Size: 294 5905 PshAI GACnn'nnGTC Cuts at: 1613 2087 1613 Size: 474 5725 PsiI TTA'TAA Cuts at: 3769 3769 Size: 6199 PstI C_TGCA'G Cuts at: 830 2664 2934 3551 830 Size: 1834 270 617 3478 Fragments arranged by size: 3478 1834 617 270 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6199 PvuII CAG'CTG Cuts at: 2789 2789 Size: 6199 SalI G'TCGA_C Cuts at: 1936 3541 1936 Size: 1605 4594 SanDI GG'GwC_CC Cuts at: 2431 2431 Size: 6199 SbfI CC_TGCA'GG Cuts at: 3551 3551 Size: 6199 ScaI AGT'ACT Cuts at: 4447 6159 4447 Size: 1712 4487 SexAI A'CCwGG_T Cuts at: 2292 2292 Size: 6199 SfcI C'TryA_G Cuts at: 826 1045 2660 2930 3136 3547 4682 5363 5554 Size: 219 1615 270 206 411 1135 681 191 Cuts at: 5554 826 Size: 1471 Fragments arranged by size: 1615 1471 1135 681 411 270 219 206 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6199 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6199 SmaI CCC'GGG Cuts at: 1872 3526 1872 Size: 1654 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2965 3083 3333 4303 5204 5466 Size: 197 926 1022 118 250 970 901 262 Cuts at: 5466 820 Size: 1553 Fragments arranged by size: 1553 1022 970 926 901 262 250 197 118 SnaBI TAC'GTA Cuts at: 493 3990 493 Size: 3497 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6199 SphI G_CATG'C Cuts at: 1132 3557 1132 Size: 2425 3774 SspI AAT'ATT Cuts at: 5 52 4123 5 Size: 47 4071 2081 Fragments arranged by size: 4071 2081 47 StuI AGG'CCT Cuts at: 1517 2612 2928 3074 1517 Size: 1095 316 146 4642 Fragments arranged by size: 4642 1095 316 146 StyI C'CwwG_G Cuts at: 513 1065 2607 2923 3471 3638 513 Size: 552 1542 316 548 167 3074 Fragments arranged by size: 3074 1542 552 548 316 167 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2359 2368 3336 4226 4243 4396 4581 Size: 1255 779 9 968 890 17 153 185 Cuts at: 4581 5026 325 Size: 445 1498 Fragments arranged by size: 1498 1255 968 890 779 445 185 153 17 9 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2556 2751 4445 Size: 274 80 33 51 1443 579 195 1694 Cuts at: 4445 6157 96 Size: 1712 138 Fragments arranged by size: 1712 1694 1443 579 274 195 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6159 6191 6159 Size: 32 6167 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6199 XbaI T'CTAG_A Cuts at: 3535 3535 Size: 6199 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6199 XmaI C'CCGG_G Cuts at: 1870 3524 1870 Size: 1654 4545 XmnI GAAnn'nnTTC Cuts at: 3000 4328 3000 Size: 1328 4871 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4007 276 Size: 53 83 186 936 2473 2468 Fragments arranged by size: 2473 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI XcmI