(Linear) (Six-base) MAPSORT of: KIEE5650.seq Check: 3924 from: 1 to: 1494 KIEE5650.orf With 182 enzymes: SgfI * December 5, 2014 13:05 .. AccI GT'mk_AC Cuts at: 0 260 526 1494 Size: 260 266 968 Fragments arranged by size: 968 266 260 AclI AA'CG_TT Cuts at: 0 1043 1494 Size: 1043 451 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 703 1494 Size: 703 791 AfeI AGC'GCT Cuts at: 0 268 1494 Size: 268 1226 AflII C'TTAA_G Cuts at: 0 1078 1494 Size: 1078 416 AflIII A'CryG_T Cuts at: 0 356 743 1494 Size: 356 387 751 Fragments arranged by size: 751 387 356 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 844 876 1494 Size: 844 32 618 Fragments arranged by size: 844 618 32 AlwNI CAG_nnn'CTG Cuts at: 0 288 447 591 723 1494 Size: 288 159 144 132 771 Fragments arranged by size: 771 288 159 144 132 AvaI C'yCGr_G Cuts at: 0 760 1494 Size: 760 734 AvrII C'CTAG_G Cuts at: 0 918 1494 Size: 918 576 BamHI G'GATC_C Cuts at: 0 1452 1494 Size: 1452 42 BanI G'GyrC_C Cuts at: 0 366 1494 Size: 366 1128 BanII G_rGCy'C Cuts at: 0 823 1494 Size: 823 671 BbsI GAAGACnn'nnnn_ Cuts at: 0 636 1064 1494 Size: 636 428 430 Fragments arranged by size: 636 430 428 BbvCI CC'TCA_GC Cuts at: 0 1153 1494 Size: 1153 341 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 871 905 1494 Size: 871 34 589 Fragments arranged by size: 871 589 34 BciVI GTATCCnnnnn_n' Cuts at: 0 523 1494 Size: 523 971 BclI T'GATC_A Cuts at: 0 936 1494 Size: 936 558 Bme1580I G_kGCm'C Cuts at: 0 371 641 1140 1494 Size: 371 270 499 354 Fragments arranged by size: 499 371 354 270 BmrI ACTGGGnnnn_n' Cuts at: 0 789 1494 Size: 789 705 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 424 1494 Size: 424 1070 Bpu10I CC'TnA_GC Cuts at: 0 1153 1494 Size: 1153 341 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 945 1313 1494 Size: 945 368 181 Fragments arranged by size: 945 368 181 BsaAI yAC'GTr Cuts at: 0 75 1494 Size: 75 1419 BsaBI GATnn'nnATC Cuts at: 0 511 941 1494 Size: 511 430 553 Fragments arranged by size: 553 511 430 BsaHI Gr'CG_yC Cuts at: 0 1091 1494 Size: 1091 403 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 844 874 1494 Size: 844 30 620 Fragments arranged by size: 844 620 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 865 898 1350 1494 Size: 865 33 452 144 Fragments arranged by size: 865 452 144 33 BseYI C'CCAG_C Cuts at: 0 63 274 281 597 1494 Size: 63 211 7 316 897 Fragments arranged by size: 897 316 211 63 7 BsiEI CG_ry'CG Cuts at: 0 132 1494 Size: 132 1362 BsiHKAI G_wGCw'C Cuts at: 0 233 1316 1494 Size: 233 1083 178 Fragments arranged by size: 1083 233 178 Bsp1286I G_dGCh'C Cuts at: 0 233 371 641 823 1140 1316 1494 Size: 233 138 270 182 317 176 178 Fragments arranged by size: 317 270 233 182 178 176 138 BspHI T'CATG_A Cuts at: 0 137 1494 Size: 137 1357 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 125 731 1494 Size: 125 606 763 Fragments arranged by size: 763 606 125 BsrFI r'CCGG_y Cuts at: 0 182 1494 Size: 182 1312 BsrGI T'GTAC_A Cuts at: 0 551 1494 Size: 551 943 BssSI C'ACGA_G Cuts at: 0 1003 1494 Size: 1003 491 BstAPI GCAn_nnn'nTGC Cuts at: 0 291 1494 Size: 291 1203 BstYI r'GATC_y Cuts at: 0 1376 1452 1494 Size: 1376 76 42 Fragments arranged by size: 1376 76 42 BstZ17I GTA'TAC Cuts at: 0 527 1494 Size: 527 967 BtgI C'CryG_G Cuts at: 0 121 1494 Size: 121 1373 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1310 1494 Size: 1310 184 DraIII CAC_nnn'GTG Cuts at: 0 291 1494 Size: 291 1203 EaeI y'GGCC_r Cuts at: 0 615 909 1494 Size: 615 294 585 Fragments arranged by size: 615 585 294 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 780 1494 Size: 780 714 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 424 703 1494 Size: 424 279 791 Fragments arranged by size: 791 424 279 EcoO109I rG'GnC_Cy Cuts at: 0 426 720 1008 1301 1494 Size: 426 294 288 293 193 Fragments arranged by size: 426 294 293 288 193 FspI TGC'GCA Cuts at: 0 241 1494 Size: 241 1253 HaeII r_GCGC'y Cuts at: 0 270 281 1195 1494 Size: 270 11 914 299 Fragments arranged by size: 914 299 270 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 404 436 1494 Size: 404 32 1058 Fragments arranged by size: 1058 404 32 HindIII A'AGCT_T Cuts at: 0 1075 1201 1239 1494 Size: 1075 126 38 255 Fragments arranged by size: 1075 255 126 38 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 311 1494 Size: 311 1183 MscI TGG'CCA Cuts at: 0 617 1494 Size: 617 877 MslI CAynn'nnrTG Cuts at: 0 491 1165 1494 Size: 491 674 329 Fragments arranged by size: 674 491 329 MspA1I CmG'CkG Cuts at: 0 95 784 1494 Size: 95 689 710 Fragments arranged by size: 710 689 95 NaeI GCC'GGC Cuts at: 0 184 1494 Size: 184 1310 NgoMIV G'CCGG_C Cuts at: 0 182 1494 Size: 182 1312 NspI r_CATG'y Cuts at: 0 360 747 1494 Size: 360 387 747 Fragments arranged by size: 747 387 360 PasI CC'CwG_GG Cuts at: 0 371 950 1494 Size: 371 579 544 Fragments arranged by size: 579 544 371 PciI A'CATG_T Cuts at: 0 356 743 1494 Size: 356 387 751 Fragments arranged by size: 751 387 356 PflMI CCAn_nnn'nTGG Cuts at: 0 288 624 1494 Size: 288 336 870 Fragments arranged by size: 870 336 288 PfoI T'CCnGG_A Cuts at: 0 422 1494 Size: 422 1072 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 844 876 1494 Size: 844 32 618 Fragments arranged by size: 844 618 32 PpuMI rG'GwC_Cy Cuts at: 0 426 720 1494 Size: 426 294 774 Fragments arranged by size: 774 426 294 PshAI GACnn'nnGTC Cuts at: 0 82 1494 Size: 82 1412 PstI C_TGCA'G Cuts at: 0 659 929 1494 Size: 659 270 565 Fragments arranged by size: 659 565 270 PvuII CAG'CTG Cuts at: 0 784 1494 Size: 784 710 SanDI GG'GwC_CC Cuts at: 0 426 1494 Size: 426 1068 SexAI A'CCwGG_T Cuts at: 0 287 1494 Size: 287 1207 SfcI C'TryA_G Cuts at: 0 655 925 1131 1494 Size: 655 270 206 363 Fragments arranged by size: 655 363 270 206 SmlI C'TyrA_G Cuts at: 0 960 1078 1328 1494 Size: 960 118 250 166 Fragments arranged by size: 960 250 166 118 StuI AGG'CCT Cuts at: 0 607 923 1069 1494 Size: 607 316 146 425 Fragments arranged by size: 607 425 316 146 StyI C'CwwG_G Cuts at: 0 602 918 1466 1494 Size: 602 316 548 28 Fragments arranged by size: 602 548 316 28 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 354 363 1331 1494 Size: 354 9 968 163 Fragments arranged by size: 968 354 163 9 TatI w'GTAC_w Cuts at: 0 551 746 1494 Size: 551 195 748 Fragments arranged by size: 748 551 195 XmnI GAAnn'nnTTC Cuts at: 0 995 1494 Size: 995 499 Enzymes that do cut: AccI AclI AcuI AfeI AflII AflIII AloI AlwNI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI Bme1580I BmrI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrFI BsrGI BssSI BstAPI BstYI BstZ17I BtgI BtgZI DraIII EaeI EciI Eco57MI EcoO109I FspI HaeII Hin4I HindIII MmeI MscI MslI MspA1I NaeI NgoMIV NspI PasI PciI PflMI PfoI PpiI PpuMI PshAI PstI PvuII SanDI SexAI SfcI SmlI StuI StyI TaqII TatI XmnI Enzymes that do not cut: AarI AatII Acc65I AgeI AhdI AleI AlfI ApaI ApaLI ApoI AscI AseI AsiSI BaeI BbeI BfrBI BglI BglII BlpI BmgBI BmtI BplI BsaI BsaWI BsgI BsiWI BsmI BsmBI BspEI BsrBI BsrDI BssHII BstBI BstEII BstXI Bsu36I BtsI ClaI CspCI DraI DrdI EagI EarI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI HincII HpaI KasI KpnI MfeI MluI NarI NcoI NdeI NheI NotI NruI NsiI PacI PmeI PmlI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI XcmI XhoI XmaI ZraI