Restriction map for
pFN21AE6649
(Linear) (Six-base) MAPSORT of: KIEE6649.seq Check: 9851 from: 1 to: 714
KIEE6649.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 419 714
Size: 40 379 295
Fragments arranged by size:
379 295 40
AleI CACnn'nnGTG
Cuts at: 0 29 391 714
Size: 29 362 323
Fragments arranged by size:
362 323 29
ApaI G_GGCC'C
Cuts at: 0 198 714
Size: 198 516
ApoI r'AATT_y
Cuts at: 0 445 714
Size: 445 269
AvrII C'CTAG_G
Cuts at: 0 483 714
Size: 483 231
BanII G_rGCy'C
Cuts at: 0 198 344 714
Size: 198 146 370
Fragments arranged by size:
370 198 146
BbvCI CC'TCA_GC
Cuts at: 0 199 714
Size: 199 515
BglI GCCn_nnn'nGGC
Cuts at: 0 515 714
Size: 515 199
Bme1580I G_kGCm'C
Cuts at: 0 198 563 714
Size: 198 365 151
Fragments arranged by size:
365 198 151
BmrI ACTGGGnnnn_n'
Cuts at: 0 200 714
Size: 200 514
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 611 714
Size: 130 481 103
Fragments arranged by size:
481 130 103
Bpu10I CC'TnA_GC
Cuts at: 0 199 714
Size: 199 515
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 655 714
Size: 237 418 59
Fragments arranged by size:
418 237 59
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 358 388 714
Size: 358 30 326
Fragments arranged by size:
358 326 30
BseYI C'CCAG_C
Cuts at: 0 146 356 714
Size: 146 210 358
Fragments arranged by size:
358 210 146
BsmI GAATG_Cn'
Cuts at: 0 124 714
Size: 124 590
Bsp1286I G_dGCh'C
Cuts at: 0 198 344 563 714
Size: 198 146 219 151
Fragments arranged by size:
219 198 151 146
BstEII G'GTnAC_C
Cuts at: 0 31 714
Size: 31 683
BstXI CCAn_nnnn'nTGG
Cuts at: 0 234 714
Size: 234 480
BstYI r'GATC_y
Cuts at: 0 50 405 714
Size: 50 355 309
Fragments arranged by size:
355 309 50
BtgI C'CryG_G
Cuts at: 0 537 714
Size: 537 177
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 124 714
Size: 124 590
BtsI GCAGTG_nn'
Cuts at: 0 119 714
Size: 119 595
EaeI y'GGCC_r
Cuts at: 0 135 714
Size: 135 579
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 130 419 611 714
Size: 40 90 289 192 103
Fragments arranged by size:
289 192 103 90 40
EcoO109I rG'GnC_Cy
Cuts at: 0 12 194 195 308 714
Size: 12 182 1 113 406
Fragments arranged by size:
406 182 113 12 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 35 67 160 192 358 390 409 441
Size: 35 32 93 32 166 32 19 32
Cuts at: 441 714
Size: 273
Fragments arranged by size:
273 166 93 35 32 32 32 32
19
MscI TGG'CCA
Cuts at: 0 137 714
Size: 137 577
MslI CAynn'nnrTG
Cuts at: 0 29 302 391 714
Size: 29 273 89 323
Fragments arranged by size:
323 273 89 29
MspA1I CmG'CkG
Cuts at: 0 631 714
Size: 631 83
NcoI C'CATG_G
Cuts at: 0 537 714
Size: 537 177
PflMI CCAn_nnn'nTGG
Cuts at: 0 159 538 634 714
Size: 159 379 96 80
Fragments arranged by size:
379 159 96 80
PfoI T'CCnGG_A
Cuts at: 0 158 714
Size: 158 556
PpuMI rG'GwC_Cy
Cuts at: 0 12 308 714
Size: 12 296 406
Fragments arranged by size:
406 296 12
PspOMI G'GGCC_C
Cuts at: 0 194 714
Size: 194 520
PvuII CAG'CTG
Cuts at: 0 631 714
Size: 631 83
SmlI C'TyrA_G
Cuts at: 0 216 670 714
Size: 216 454 44
Fragments arranged by size:
454 216 44
StuI AGG'CCT
Cuts at: 0 594 714
Size: 594 120
StyI C'CwwG_G
Cuts at: 0 483 537 714
Size: 483 54 177
Fragments arranged by size:
483 177 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 348 714
Size: 348 366
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 455 487 513 545 714
Size: 455 32 26 32 169
Fragments arranged by size:
455 169 32 32 26
Tth111I GACn'n_nGTC
Cuts at: 0 172 714
Size: 172 542
Enzymes that do cut:
AcuI AleI ApaI ApoI AvrII BanII BbvCI BglI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaXI BseYI BsmI
Bsp1286I BstEII BstXI BstYI BtgI BtgZI BtsI EaeI
Eco57MI EcoO109I Hin4I MscI MslI MspA1I NcoI PflMI
PfoI PpuMI PspOMI PvuII SmlI StuI StyI TaqII
TstI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaLI AscI AseI
AsiSI AvaI BaeI BamHI BanI BbeI BbsI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EagI EarI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TatI XbaI XcmI XhoI XmaI
XmnI ZraI