(Linear) (Six-base) MAPSORT of: KIEE5754.seq Check: 2846 from: 1 to: 2208 KIEE5754.orf With 182 enzymes: SgfI * December 5, 2014 13:09 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 877 2051 2208 Size: 877 1174 157 Fragments arranged by size: 1174 877 157 AflII C'TTAA_G Cuts at: 0 432 2208 Size: 432 1776 AflIII A'CryG_T Cuts at: 0 363 2208 Size: 363 1845 AhdI GACnn_n'nnGTC Cuts at: 0 1837 2208 Size: 1837 371 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 780 2208 Size: 780 1428 ApoI r'AATT_y Cuts at: 0 284 469 475 623 882 966 1015 1046 Size: 284 185 6 148 259 84 49 31 Cuts at: 1046 1092 1222 1496 2181 2208 Size: 46 130 274 685 27 Fragments arranged by size: 685 284 274 259 185 148 130 84 49 46 31 27 6 AseI AT'TA_AT Cuts at: 0 121 2208 Size: 121 2087 AvaI C'yCGr_G Cuts at: 0 902 1545 2208 Size: 902 643 663 Fragments arranged by size: 902 663 643 BamHI G'GATC_C Cuts at: 0 298 897 2208 Size: 298 599 1311 Fragments arranged by size: 1311 599 298 BciVI GTATCCnnnnn_n' Cuts at: 0 785 2208 Size: 785 1423 BclI T'GATC_A Cuts at: 0 99 2208 Size: 99 2109 BglII A'GATC_T Cuts at: 0 373 543 2208 Size: 373 170 1665 Fragments arranged by size: 1665 373 170 BlpI GC'TnA_GC Cuts at: 0 1688 2208 Size: 1688 520 BmgBI CAC'GTC Cuts at: 0 697 2208 Size: 697 1511 BmrI ACTGGGnnnn_n' Cuts at: 0 1245 2114 2208 Size: 1245 869 94 Fragments arranged by size: 1245 869 94 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1584 1619 2132 2208 Size: 1584 35 513 76 Fragments arranged by size: 1584 513 76 35 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1102 2208 Size: 1102 1106 BsaAI yAC'GTr Cuts at: 0 974 1296 2208 Size: 974 322 912 Fragments arranged by size: 974 912 322 BsaBI GATnn'nnATC Cuts at: 0 923 1535 2208 Size: 923 612 673 Fragments arranged by size: 923 673 612 BsaHI Gr'CG_yC Cuts at: 0 128 2208 Size: 128 2080 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 2081 2208 Size: 2081 127 BsmBI CGTCTCn'nnnn_ Cuts at: 0 545 2208 Size: 545 1663 BsrDI GCAATG_nn' Cuts at: 0 752 2208 Size: 752 1456 BsrGI T'GTAC_A Cuts at: 0 1397 2208 Size: 1397 811 BstAPI GCAn_nnn'nTGC Cuts at: 0 636 2208 Size: 636 1572 BstYI r'GATC_y Cuts at: 0 298 373 543 897 1933 2208 Size: 298 75 170 354 1036 275 Fragments arranged by size: 1036 354 298 275 170 75 BtsI GCAGTG_nn' Cuts at: 0 2024 2208 Size: 2024 184 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 347 382 2208 Size: 347 35 1826 Fragments arranged by size: 1826 347 35 DraI TTT'AAA Cuts at: 0 1020 2208 Size: 1020 1188 DraIII CAC_nnn'GTG Cuts at: 0 349 1723 1808 2208 Size: 349 1374 85 400 Fragments arranged by size: 1374 400 349 85 EaeI y'GGCC_r Cuts at: 0 838 2208 Size: 838 1370 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1565 2208 Size: 1565 643 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 877 1584 1619 2051 2132 2208 Size: 877 707 35 432 81 76 Fragments arranged by size: 877 707 432 81 76 35 EcoNI CCTnn'n_nnAGG Cuts at: 0 1661 2208 Size: 1661 547 EcoO109I rG'GnC_Cy Cuts at: 0 2016 2208 Size: 2016 192 EcoRI G'AATT_C Cuts at: 0 966 2208 Size: 966 1242 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1363 1395 1823 1855 2128 2160 2187 2208 Size: 1363 32 428 32 273 32 27 21 Fragments arranged by size: 1363 428 273 32 32 32 27 21 HincII GTy'rAC Cuts at: 0 43 1353 1773 2208 Size: 43 1310 420 435 Fragments arranged by size: 1310 435 420 43 MfeI C'AATT_G Cuts at: 0 769 2208 Size: 769 1439 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 734 1149 1182 2110 2208 Size: 734 415 33 928 98 Fragments arranged by size: 928 734 415 98 33 MscI TGG'CCA Cuts at: 0 840 2208 Size: 840 1368 MslI CAynn'nnrTG Cuts at: 0 416 1753 1892 2208 Size: 416 1337 139 316 Fragments arranged by size: 1337 416 316 139 NdeI CA'TA_TG Cuts at: 0 1368 2208 Size: 1368 840 NspI r_CATG'y Cuts at: 0 367 717 2208 Size: 367 350 1491 Fragments arranged by size: 1491 367 350 PasI CC'CwG_GG Cuts at: 0 1261 1875 2208 Size: 1261 614 333 Fragments arranged by size: 1261 614 333 PciI A'CATG_T Cuts at: 0 363 2208 Size: 363 1845 PflMI CCAn_nnn'nTGG Cuts at: 0 180 1759 2208 Size: 180 1579 449 Fragments arranged by size: 1579 449 180 PpuMI rG'GwC_Cy Cuts at: 0 2016 2208 Size: 2016 192 PsiI TTA'TAA Cuts at: 0 466 2208 Size: 466 1742 PstI C_TGCA'G Cuts at: 0 1494 2208 Size: 1494 714 SfcI C'TryA_G Cuts at: 0 303 440 1490 1661 1778 2208 Size: 303 137 1050 171 117 430 Fragments arranged by size: 1050 430 303 171 137 117 SmlI C'TyrA_G Cuts at: 0 432 1081 2208 Size: 432 649 1127 Fragments arranged by size: 1127 649 432 SnaBI TAC'GTA Cuts at: 0 974 1296 2208 Size: 974 322 912 Fragments arranged by size: 974 912 322 StyI C'CwwG_G Cuts at: 0 726 1311 1737 2208 Size: 726 585 426 471 Fragments arranged by size: 726 585 471 426 TatI w'GTAC_w Cuts at: 0 315 1194 1397 2208 Size: 315 879 203 811 Fragments arranged by size: 879 811 315 203 XbaI T'CTAG_A Cuts at: 0 1158 2208 Size: 1158 1050 XmnI GAAnn'nnTTC Cuts at: 0 1049 1499 2208 Size: 1049 450 709 Fragments arranged by size: 1049 709 450 Enzymes that do cut: AcuI AflII AflIII AhdI AlfI ApoI AseI AvaI BamHI BciVI BclI BglII BlpI BmgBI BmrI BpmI BpuEI BsaAI BsaBI BsaHI BseRI BsmBI BsrDI BsrGI BstAPI BstYI BtsI CspCI DraI DraIII EaeI EciI Eco57MI EcoNI EcoO109I EcoRI Hin4I HincII MfeI MmeI MscI MslI NdeI NspI PasI PciI PflMI PpuMI PsiI PstI SfcI SmlI SnaBI StyI TatI XbaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AgeI AleI AloI AlwNI ApaI ApaLI AscI AsiSI AvrII BaeI BanI BanII BbeI BbsI BbvCI BcgI BfrBI BglI Bme1580I BmtI BplI Bpu10I BsaI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI DrdI EagI EarI EcoICRI EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MluI MspA1I NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PfoI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SpeI SphI SrfI SspI StuI SwaI TaqII TstI Tth111I XcmI XhoI XmaI ZraI