Restriction map for
pFN21AE5753
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# NO CUT ENZYMES ( 136 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII BbeI BbrPI BbvCI
BcgI BclI BsaBI BsaHI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I CfoI Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AccI 145 GTAGAC GT^[AC][GT]AC
AflII 1084 CTTAAG C^TTAAG
AflIII 1138 ACGTGT A^C[GA][CT]GT
AocI 1962 CCTGAGG CC^T[ACGT]AGG
BalI 805 TGGCCA TGG^CCA
BamHI 354 GGATCC G^GATCC
BanII 1600 GAGCCC G[GA]GC[CT]^C
BfrI 1084 CTTAAG C^TTAAG
BglII 112 AGATCT A^GATCT
BsaAI 1137 TACGTG [CT]AC^GT[GA]
BsgI 532 GTGCAG GTGCAG(16/14)
BspHI 1125 TCATGA T^CATGA
BsrDI 321 CATTGC GCAATG(2/0)
Bsu36I 1962 CCTGAGG CC^T[ACGT]AGG
DraI 585 TTTAAA TTT^AAA
EaeI 805 TGGCCA [CT]^GGCC[GA]
Eco81I 1962 CCTGAGG CC^T[ACGT]AGG
HindIII 124 AAGCTT A^AGCTT
MluNI 805 TGGCCA TGG^CCA
MscI 805 TGGCCA TGG^CCA
MspA1I 334 CAGCTG C[AC]G^C[GT]G
PpuMI 1996 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 334 CAGCTG CAG^CTG
RcaI 1125 TCATGA T^CATGA
ScaI 1015 AGTACT AGT^ACT
TaqI 2016 TCGA T^CGA
TthHB8I 2016 TCGA T^CGA
XcmI 2035 CCAGATGATGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AciI 1289 CCGC CCGC(-3/-1)
926 GCGG
AvrII 420 CCTAGG C^CTAGG
1099 CCTAGG
BbsI 1241 GTCTTC GAAGAC(2/6)
1949 GTCTTC
BciVI 3 GGATAC GTATCC(6/5)
1284 GGATAC
BcnI 372 CCCGG CC^[GC]GG
1466 CCGGG
BglI 551 GCCTCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1307 GCCAAACAGGC
BlnI 420 CCTAGG C^CTAGG
1099 CCTAGG
BlpI 448 GCTCAGC GC^T[ACGT]AGC
661 GCTGAGC
Bpu1102I 448 GCTCAGC GC^T[ACGT]AGC
661 GCTGAGC
BpuAI 1241 GTCTTC GAAGAC(2/6)
1949 GTCTTC
BsmI 166 GCATTC GAATGC(1/-1)
1434 GCATTC
BspMI 39 ACCTGC ACCTGC(4/8)
475 ACCTGC
Cac8I 293 GCAAGC GC[ACGT]^[ACGT]GC
1258 GCTGGC
CelII 448 GCTCAGC GC^T[ACGT]AGC
661 GCTGAGC
DraII 1853 AGGGCCT [GA]G^G[ACGT]CC[CT]
1996 GGGTCCC
DsaI 808 CCATGG C^C[GA][CT]GG
1589 CCATGG
EcoO109I 1853 AGGGCCT [GA]G^G[ACGT]CC[CT]
1996 GGGTCCC
EcoRI 213 GAATTC G^AATTC
1977 GAATTC
HapII 373 CCGG C^CGG
1466 CCGG
HpaII 373 CCGG C^CGG
1466 CCGG
MaeII 1138 ACGT A^CGT
1445 ACGT
MslI 194 CACCAGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1135 CATACGTGTG
MspI 373 CCGG C^CGG
1466 CCGG
NciI 372 CCCGG CC^[GC]GG
1466 CCGGG
NcoI 808 CCATGG C^CATGG
1589 CCATGG
PstI 1319 CTGCAG CTGCA^G
2158 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI AvaII BanI BbvI BfaI
BmyI BpmI BsaI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
BstYI Cfr13I DdeI DpnII EarI
EcoNI EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII